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An approach toward establishing Agrobacterium-mediated transformation system of mitochondria

Research Project

Project/Area Number 23658002
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Breeding science
Research InstitutionTohoku University

Principal Investigator

TORIYAMA Kinya  東北大学, 大学院・農学研究科, 教授 (20183882)

Co-Investigator(Renkei-kenkyūsha) ITO Yukihiro  東北大学, 大学院・農学研究科, 准教授 (70280576)
KAZAMA Tomohiko  東北大学, 大学院・農学研究科, 助教 (30431464)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物
Research Abstract

To establish transformation system of plant mitochondria, we aimed to produce genetically modified Agrobacterium so that it delivered T-DNA into mitochondria. To deliver T-DNA into mitochondria, we removed the nuclear localization signal sequence from VirD2, which usually played a role to deliver T-DNA into nucleus, and added a mitochondrial targeting signal sequence. Finally we produced Agrobacterium carrying modified VirD2 with mitochondrial targeting signal sequence.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (42 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (21 results) (of which Invited: 3 results) Book (1 results) Remarks (15 results)

  • [Journal Article] Re-sequencing of mitochondrial genes in a standard rice cultivar Nipponbare.2013

    • Author(s)
      戸田拓士
    • Journal Title

      RICE

      Volume: 6 Issue: 1 Pages: 2-2

    • DOI

      10.1186/1939-8433-6-2

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The fertility restorer gene, Rf2, for Lead Rice-type cytoplasmic male sterility of rice encodes a mitochondrial glycine-rich protein2011

    • Author(s)
      Itabashi, E., Iwata, N., Fujii, S., Kazama, T., Toriyama, K
    • Journal Title

      Plant J

      Volume: 65 Issue: 3 Pages: 359-367

    • DOI

      10.1111/j.1365-313x.2010.04427.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptome map of plant mitochondria reveals islands of unexpected transcribed regions2011

    • Author(s)
      Fujii S, Toda T, Kikuchi S, Suzuki R, Yokoyama K, Tsuchida H, Yano K
    • Journal Title

      BMC Genomics

      Volume: 12 Issue: 1 Pages: 279-279

    • DOI

      10.1186/1471-2164-12-279

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic regulation of sporopollenin synthesis and pollen exine development2011

    • Author(s)
      Ariizumi T, Toriyama K
    • Journal Title

      Annu Rev Plant Biol

      Volume: 62 Issue: 1 Pages: 437-460

    • DOI

      10.1146/annurev-arplant-042809-112312

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica2011

    • Author(s)
      Y.Honma
    • Journal Title

      Current Genetics

      Volume: 57 Issue: 4 Pages: 261-270

    • DOI

      10.1007/s00294-011-0345-3

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Genome barriers between mitochondria andnuclei exemplified by cytoplasmic male sterility/fertility restoration in rice2012

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      10th International Congress on PlantMolecular Biology
    • Place of Presentation
      Jeju, Korea(招待)
    • Related Report
      2012 Final Research Report
  • [Presentation] イネにおけるミトコンドリア遺伝性の花粉発育不全とそれをレスキューする核遺伝子2012

    • Author(s)
      鳥山欽哉
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      福岡市九州大学医学部(招待)
    • Related Report
      2012 Final Research Report
  • [Presentation] コメ産業の国際化を狙った新規ハイブリッドライス育種基盤の開発2012

    • Author(s)
      鳥山欽哉・風間智彦
    • Organizer
      日本育種学会2012年秋季大会
    • Place of Presentation
      京都市京都産業大学(招待)
    • Related Report
      2012 Final Research Report
  • [Presentation] Fertility restoration in three types of cytoplasmic male sterility in rice2012

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      XXII International Congress on Sexual Plant Reproduction (Plant Reproduction for Food)
    • Place of Presentation
      Melbourne, Australia(招待)
    • Related Report
      2012 Final Research Report
  • [Presentation] イネにおけるミトコンドリア遺伝性の花粉発育不全とそれをレスキューする核遺伝子2012

    • Author(s)
      鳥山欽哉・風間智彦
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] コメ産業の国際化を狙った新規ハイブリッドライス育種基盤の開発2012

    • Author(s)
      鳥山欽哉・風間智彦
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学(京都市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Genome barriers between mitochondria and nuclei exemplified by cytoplasmic male sterility/fertility restoration in rice2012

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Fertility restoration in three types of cytoplasmic male sterility in rice2012

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      XXII International Congress on Sexual Plant Reproduction (Plant Reproduction for Food)(招待講演)
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular analysis of partial fertility restorer gene, Rf2, for BT-type cytoplasmic male sterile rice2012

    • Author(s)
      Kazama T, Itabashi E, Toriyama K
    • Organizer
      XXII International Congress on Sexual Plant Reproduction (Plant Reproduction for Food)
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2011 Research-status Report
  • [Presentation] 細胞質雄性不稔性イネミトコンドリアにおける稔性回復因子RF2によるORF79の翻訳制御2012

    • Author(s)
      風間智彦, 板橋悦子, 藤井慎也, 鳥山欽哉
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都市
    • Related Report
      2011 Research-status Report
  • [Presentation] New resources for hybrid rice breeding2011

    • Author(s)
      Toriyama K
    • Organizer
      XVIII International Botanical Congress
    • Place of Presentation
      Melbourne, Australia(招待)
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular analysis of cytoplasmic male sterility and fertility restoration in rice2011

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      1st Congress of Cereal Biotechnology andBreeding
    • Place of Presentation
      Szeged, Hungary(キーノートスピーカー)
    • Related Report
      2012 Final Research Report
  • [Presentation] Rice mitochondrial PPR25 (MPR25) is not only essential for RNA editing of nad5 transcript in mitochondrial but for chloroplast function2011

    • Author(s)
      Toda T, Fujii S, Noguchi K, Kazama T, Toriyama K
    • Organizer
      International Conference for Plant Mitochondrial Biology
    • Place of Presentation
      Fulda, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular analysis of cytoplasmic male sterility and fertility restoration in rice.2011

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      1st Congress of Cereal Biotechnology and Breeding(招待講演)
    • Place of Presentation
      Szeged, Hungary
    • Related Report
      2011 Research-status Report
  • [Presentation] New resources for hybrid rice breeding2011

    • Author(s)
      Toriyama K
    • Organizer
      XVIII International Botanical Congress(招待講演)
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2011 Research-status Report
  • [Presentation] Comparative analysis of fertility restoration mechanism in BT-CMS/RF1, CW-CMS/RF17 and LD-CMS/RF22011

    • Author(s)
      Toriyama K, Kazama T
    • Organizer
      9th International Symposium of Rice Functional Genomics
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of a cytoplasmic male sterile rice RT102A and mapping of its restorer of fertility gene2011

    • Author(s)
      Murata H, Toriyama K, Motomura K, Kazama T
    • Organizer
      9th International Symposium of Rice Functional Genomics
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2011 Research-status Report
  • [Presentation] 生育初期の光合成に影響を及ぼすイネミトコンドリア局在PPR mpr25変異体の解析2011

    • Author(s)
      戸田拓士・藤井壮太・野口航・有村慎一・風間智彦・鳥山欽哉
    • Organizer
      第29回日本植物細胞分子生物学会
    • Place of Presentation
      福岡市
    • Related Report
      2011 Research-status Report
  • [Presentation] 光合成に影響を及ぼすイネミトコンドリアPPR MPR25の機能解析2011

    • Author(s)
      戸田拓士・藤井壮太・野口航・有村慎一・風間智彦・鳥山欽哉
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      福井市
    • Related Report
      2011 Research-status Report
  • [Presentation] 雄性不稔細胞質を持つ野生イネ8系統のミトコンドリアゲノム構造と稔性回復遺伝子の比較2011

    • Author(s)
      加納孝紘・風間智彦・鳥山欽哉
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      福井市
    • Related Report
      2011 Research-status Report
  • [Presentation] 雄性不稔細胞質を持つRT102Cにおける稔性回復遺伝子のマッピング2011

    • Author(s)
      村田隼人・風間智彦・本村恵二・鳥山欽哉
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      福井市
    • Related Report
      2011 Research-status Report
  • [Book] 農学生命科学を学ぶための入門生物学2011

    • Author(s)
      山口高弘・鳥山欽哉編
    • Publisher
      東北大学出版会
    • Related Report
      2011 Research-status Report
  • [Remarks] (1)伊藤幸博東北・北海道地区SSH指定校発表会指導助言講師(宮城県仙台第三高等学校)2013年1月26日-27日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (2)伊藤幸博植物の多様な生存戦略~東北に植物のチカラを~日本植物生理学会高校生シンポジウム組織委員副委員長(東北大学)2012年8月6日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (3)鳥山欽哉エンジョイDNA~よくわかる遺伝子組換え植物~市民講座東北大学サイエンスカフェ第82回(仙台メディアテーク)2012年7月27日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (4)伊藤幸博DNAと遺伝子組換え植物次世代型科学者の卵養成講座(東北大学)2012年7月14日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (5)鳥山欽哉エンジョイDNA~遺伝子組換え植物と植物の環境適応福島県立磐城高等学校模擬講義(東北大学)2012年4月20日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (6)伊藤幸博エンジョイDNA~遺伝子組換え植物と植物の環境適応福島県立磐城高等学校模擬講義(東北大学)2012年4月20日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (7)伊藤幸博DNAと遺伝子組換え植物第11回東北大学出前授業(仙台市立東二番丁小学校)2011年11月22日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (8)鳥山欽哉エンジョイDNA~遺伝子組換え植物と植物の環境適応第4回科学者の卵養成講座(東北大学)2011年9月3日 ○アウトリーチ活動

    • Related Report
      2012 Final Research Report
  • [Remarks] (1)鳥山欽哉東北大学サイエンスカフェから細菌を利用して植物を改良河北新報2012年8月8日 ○新聞報道

    • Related Report
      2012 Final Research Report
  • [Remarks] (1)伊藤幸博・鳥山欽哉インターネット上での研究成果の継続的な発信「環境適応生物工学研究室お知らせと更新情報」

    • URL

      http://www.agri.tohoku.ac.jp/bioadp/PukiWiki/index.php?FrontPage

    • Related Report
      2012 Final Research Report
  • [Remarks] (2)鳥山欽哉エンジョイDNA~よくわかる遺伝子組み換え植物~,東北大学サイエンスカフェ動画チャンネル公開日2012年10月4日

    • URL

      http://www.youtube.com/watch?v=u6gU81x0VAQ&feature=youtu.be

    • Related Report
      2012 Final Research Report
  • [Remarks] 環境適応生物工学研究室

    • URL

      http://www.agri.tohoku.ac.jp/bioadp/PukiWiki/index.php?FrontPage

    • Related Report
      2012 Annual Research Report
  • [Remarks] 東北大学サイエンスカフェ「エンジョイDNA~よくわかる遺伝子組み換え植物~」

    • URL

      http://www.youtube.com/watch?v=u6gU81x0VAQ&feature=youtu.be

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/bioadp/PukiWiki/index.php?FrontPage

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://bioinf.mind.meiji.ac.jp/cgi-bin/gbrowse/OsMes/#search

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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