| Project/Area Number |
23658002
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Breeding science
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| Research Institution | Tohoku University |
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Principal Investigator |
TORIYAMA Kinya 東北大学, 大学院・農学研究科, 教授 (20183882)
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| Co-Investigator(Renkei-kenkyūsha) |
ITO Yukihiro 東北大学, 大学院・農学研究科, 准教授 (70280576)
KAZAMA Tomohiko 東北大学, 大学院・農学研究科, 助教 (30431464)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物 |
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| Research Abstract |
To establish transformation system of plant mitochondria, we aimed to produce genetically modified Agrobacterium so that it delivered T-DNA into mitochondria. To deliver T-DNA into mitochondria, we removed the nuclear localization signal sequence from VirD2, which usually played a role to deliver T-DNA into nucleus, and added a mitochondrial targeting signal sequence. Finally we produced Agrobacterium carrying modified VirD2 with mitochondrial targeting signal sequence.
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