Analysis of microRNA transcription machinery and its application for artificial miRNA gene expression.

• Project/Area Number: 23658006
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: Nagoya University
• Principal Investigator: SATO Yutaka 名古屋大学, 生命農学研究科, 准教授 (40345872)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物育種・遺伝 / バイオテクノロジー / miRNA / イネ / DNA依存的RNA合成酵素

Research Abstract

The goal of this research is to understand the molecular mechanism of miRNA gene transcription in rice and to develop a system that ensure the high level of amiRNA gene expression. Using the knock down lines of genes encoding DNA dependent RNA polymerase subunit, I found that several miRNA gene expressions were decreased as well as the changes in the expression of some cell cycle regulation genes.

Research Products

• [Journal Article] Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice. (2013)
  • Author(s): Ishiwata A, Ozawa M, Nagasaki H, Kato M, Noda Y, Yamaguchi T, Nosaka M, Shimizu-Sato S, Nagasaki A, Maekawa M, Hirano HY, Sato Y
  • Journal Title: Plant Cell Physiology Volume: (印刷中)(in press) Issue: 5 Pages: 779-792
  • DOI: 10.1093/pcp/pct032
  • Peer Reviewed
• [Journal Article] Expression of the rice microRNA miR820 is associated with epigenetic modifications at its own locus (2013)
  • Author(s): Misuzu Nosaka, Akemi Ono, Aiko Ishiwata, Sae Shimizu-Sato, Kiyoe Ishimoto, Yusaku Noda and Yutaka Sato
  • Journal Title: Genes Genet. Syst
  • NAID: 10031183176
  • Peer Reviewed
• [Journal Article] The copy number of rice CACTA DNA transposon carrying MIR820 does not correlate with MIR820 expression (2013)
  • Author(s): Misuzu Nosaka, Aiko Ishiwata, Sae-Shimizu Sato, Akemi Ono, Kiyoe Ishimoto, Yusaku Noda, Yutaka Sato
  • Journal Title: Plant Signal. Behav
  • Peer Reviewed
• [Journal Article] 小分子 RNA を介したゲノム寄生因子と宿主ゲノムの攻防「生命分子を統合する RNA-その秘められた役割と制御機構」 (2013)
  • Author(s): 佐藤 豊
  • Journal Title: 実験医学 Volume: 31(7) Pages: 91-97
• [Journal Article] Role of transposon-derived small RNAs in the interplay between genomes and parasitic DNA in rice (2012)
  • Author(s): Misuzu Nosaka, Jun-ichi Itoh, Yasuo Nagato, Akemi Ono, Aiko Ishiwata, Yutaka Sato
  • Journal Title: PLoS Genetics Volume: 8(9) Issue: 9 Pages: e1002953-e1002953
  • DOI: 10.1371/journal.pgen.1002953
  • Peer Reviewed
• [Journal Article] Role of transposon-derived small RNAs in the interplay between genomes and parasitic DNA in rice. (2012)
  • Author(s): Misuzu Nosaka
  • Journal Title: PLoS Genetics Volume: 8
  • Peer Reviewed
• [Journal Article] LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the formation of axillary meristems (2011)
  • Author(s): Hiroaki Tabuchi, Yu Zhang, Susumu Hattori, Minami Omae, Sae Shimizu-Sato, Tetsuo Oikawa, Qian Qian, Minoru Nishimura, Hidemi Kitano, He Xie, Xiaohua Fang, Hitoshi Yoshida, Junko Kyozuka, Fan Chen, and Yutaka Sato
  • Journal Title: Plant Cell Volume: 23 Pages: 3276-3287
  • Peer Reviewed
• [Journal Article] RSS1 regulates the cell cycle and maintains meristematic activity under stress conditions in rice. (2011)
  • Author(s): Daisuke Ogawa, , Hitoshi Sakakibara, Yutaka Sato, Shin Takeda
  • Journal Title: Nature Communications Volume: 2 Pages: 278-278
  • Peer Reviewed
• [Journal Article] LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the formation of axillary meristems. (2011)
  • Author(s): Hiroaki Tabuchi, , Hidemi Kitano, Hitoshi Yoshida, Junko Kyozuka, Fan Chen, Yutaka Sato
  • Journal Title: The Plant Cell Volume: 23 Pages: 3276-3287
  • Peer Reviewed
• [Journal Article] Positive autoregulation of a KNOX gene is essential for shoot meristem maintenance in rice. (2011)
  • Author(s): Katsutoshi Tsuda, Yutaka Sato, Yukihiro Itoh, Nori Kurata
  • Journal Title: The Plant Cell Volume: 23 Pages: 4368-4381
  • Peer Reviewed
• [Presentation] Battles between plant genome and its parasitic elements through the action of small RNAs (2013)
  • Author(s): Yutaka Sato
  • Organizer: Japan-China rice developmental biology meeting-Morphology to Yield
  • Place of Presentation: Beppu, Oita, Japan
• [Presentation] 小分子RNAによる植物のゲノム動態制御とその利用 (2012)
  • Author(s): 佐藤豊
  • Organizer: さきがけ交流会
  • Place of Presentation: ウィル名古屋
  • Year and Date: 2012-08-09
• [Presentation] An anti-host strategy of transposons through the action of small RNAs (2011)
  • Author(s): Yutaka Sato
  • Organizer: MBSJ workshop
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2011-12-14
• [Presentation] 植物の小分子RNAを介したゲノム間相互作用の解析とその応用 (2011)
  • Author(s): 佐藤 豊
  • Organizer: さきがけ「RNAと生体機能」第三回研究報告会
  • Place of Presentation: 東大弥生講堂
  • Year and Date: 2011-12-12
• [Presentation] RNAワールドの今 (2011)
  • Author(s): 佐藤 豊
  • Organizer: 遺伝学研究所研究会「イネ分子遺伝学の飛躍」
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2011-11-18
• [Presentation] Battles between plant genome and its parasitic elements through the action of small RNAs (2011)
  • Author(s): Yutaka Sato
  • Organizer: NTU-JST Joint Symposium on RNA and Biofunctions-Asia Studies
  • Place of Presentation: Taipei Taiwan
  • Year and Date: 2011-11-10
• [Presentation] Battles between plant genome and its parasitic elements through the action of small RNAs (2011)
  • Author(s): Yutaka Sato
  • Organizer: Tokyo RNA Club
  • Place of Presentation: Keio University
  • Year and Date: 2011-09-14
• [Patent(Industrial Property Rights)] 害虫防除法 (2012)
  • Inventor(s): 新美輝幸、吉岡博史、佐藤豊
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2012-01-02
  • Overseas
• [Patent(Industrial Property Rights)] 害虫防除法 (2011)
  • Inventor(s): 新美輝幸、吉岡博史、佐藤豊
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2011-09-28
  • Overseas
• [Patent(Industrial Property Rights)] 害虫防除法 (2011)
  • Inventor(s): 新美輝幸、吉岡博史、佐藤豊
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2011-12-02
  • Overseas