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Analysis of proteins specifically produced in the tissues during aerenchyma formation in maize

Research Project

Project/Area Number 23658008
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Breeding science
Research InstitutionNagoya University

Principal Investigator

NAKAZONO Mikio  名古屋大学, 生命農学研究科, 教授 (70282697)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsレーザーマイクロダイセクション / タンパク質 / 通気組織 / プロテオーム
Research Abstract

Aerenchyma enables internal aeration between shoots and roots, and its formation is therefore important for the adaptation of plants to excess water environments. I examined optimal conditions for comprehensive proteome analysis using the cortex isolated by laser microdissection (LM) from maize roots. I determined an optimal fixative for fixation of tissues. Moreover, I detected Alcohol dehydrogenase protein by western blotting using LM-isolated cortex proteins. However, I failed to obtain enough amounts of proteins that are required for comprehensive proteome analysis.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (14 results)

All 2012 2011

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (4 results)

  • [Journal Article] Enhanced formation of aerenchyma and induction of a barrier to radial oxygen loss in adventitious roots of Zea nicaraguensis contribute to its waterlogging tolerance as compared with maize (Zea mays ssp.mays.)2012

    • Author(s)
      Abiko, T., Kotula, L., Shiono, K., Malik, A.I., Colmer, T.D., Nakazono, M.
    • Journal Title

      Plant Cell Environ

      Volume: (in press) Issue: 9 Pages: 1618-1630

    • DOI

      10.1111/j.1365-3040.2012.02513.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of candidate genes associated with constitutive aerenchyma formation in adventitious roots of the teosinte <I>Zea nicaraguensis</I>2012

    • Author(s)
      Abiko, T., Obara, M., Abe, F., Kawaguchi, K., Oyanagi, A., Yamauchi, T. and Nakazono, M
    • Journal Title

      Plant Root

      Volume: 6 Issue: 0 Pages: 19-27

    • DOI

      10.3117/plantroot.6.19

    • NAID

      130002077479

    • ISSN
      1881-6754
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] AhNRAMP1 iron transporter is involved in iron acquisition in peanut2012

    • Author(s)
      Xiong H, Kobayashi T, Kakei Y, Senoura T, Nakazono M, Takahashi H, Nakanishi H, Shen H, Duan P, Guo X, Nishizawa NK,* Zuo Y,*
    • Journal Title

      Journal of Experimental Botany

      Volume: 63 Issue: 12 Pages: 4437-4446

    • DOI

      10.1093/jxb/ers117

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue-specific transcriptome analysis in nodules of Lotus japonicus2012

    • Author(s)
      Takanashi, K., et al
    • Journal Title

      Mol Plant Microbe Interact.

      Volume: 25(in press) Issue: 7 Pages: 869-876

    • DOI

      10.1094/mpmi-01-12-0011-r

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acquisition of aluminum tolerance by modification of a single gene in barley2012

    • Author(s)
      Fujii M., Yokosho K., Yamaji N., Saisho D., Yamane M., Takahashi H., Sato K., Nakazono M., Ma J.F.
    • Journal Title

      Nature Communications

      Volume: Vol.3 Issue: 1 Pages: 713-713

    • DOI

      10.1038/ncomms1726

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide transcriptome dissection of the rice root system: Implications for developmental and physiological functions2012

    • Author(s)
      Takehisa, H., Sato, Y., Igarashi, M., Abiko, T., Antonio, B., Kamatsuki, K., Minami, H., Namiki, N., Inukai, Y., Nakazono, M. and Nagamura, Y
    • Journal Title

      Plant Journal

      Volume: 69 Issue: 1 Pages: 126-140

    • DOI

      10.1111/j.1365-313x.2011.04777.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses2011

    • Author(s)
      Rajhi I., Yamauchi T., Takahashi H., Nishiuchi S., Shiono K., Watanabe R., Mliki A., Nagamura Y., Tsutsumi N., Nishizawa N., Nakazono M.
    • Journal Title

      NEW PHYTOLOGIST

      Volume: 190 Issue: 2 Pages: 351-368

    • DOI

      10.1111/j.1469-8137.2010.03535.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive network analysis of anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays.2011

    • Author(s)
      Aya, et al.
    • Journal Title

      PLoS One

      Volume: 6 Issue: 10 Pages: e26162-e26162

    • DOI

      10.1371/journal.pone.0026162

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence2011

    • Author(s)
      Takahashi H., Saika H., Matsumura H, Nagamura Y., Tsutsumi N., Nishizawa N., Nakazono M.
    • Journal Title

      ANNALS OF BOTANY

      Volume: 108 Issue: 2 Pages: 253-261

    • DOI

      10.1093/aob/mcr137

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contrasting dynamics of radial O2-loss barrier induction and aerenchyma formation in rice roots of two lengths2011

    • Author(s)
      Shiono, K., Ogawa, S., Yamazaki, S., Isoda, H., Fujimura, T., Nakazono, M. and Colmer, T.D
    • Journal Title

      Annals of Botany

      Volume: 107 Issue: 1 Pages: 89-99

    • DOI

      10.1093/aob/mcq221

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] トウモロコシにおける通気組織形成時のエチレン応答性遺伝子の発現解析2012

    • Author(s)
      高橋宏和、山内卓樹、Imene Rajhi、長村吉晃、堤伸浩、西澤直子、中園幹生
    • Organizer
      第122回 日本育種学会講演会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] トウモロコシにおける通気組織形成時のエチレン応答性遺伝子の発現解析2012

    • Author(s)
      高橋宏和
    • Organizer
      第122回 日本育種学会講演会
    • Place of Presentation
      京都産業大学(京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] トウモロコシの通気組織形成に関与するエチレン応答性遺伝子の探索2011

    • Author(s)
      高橋宏和、山内卓樹、Imene. Rajhi、長村吉晃、堤伸浩、西澤直子、中園幹生
    • Organizer
      第120回 日本育種学会講演会
    • Place of Presentation
      福井
    • Related Report
      2012 Final Research Report
  • [Presentation] Study of aerenchyma formation in maize roots during waterlogged conditions. Tunisian Japanese Symposium on Science2011

    • Author(s)
      Imene Rajhi, Takaki Yamauchi, Hirokazu Takahashi, Shunsaku Nishiuchi, Katsuhiro Shiono, Ryosuke Watanabe, Ahmed Mliki, Yoshiaki Nagamura, Nobuhiro Tsutsumi, Naoko K. Nishizawa, Mikio Nakazono
    • Organizer
      Society and Technology 11
    • Place of Presentation
      Yasmine Hammamet, Tunisia.
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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