Project/Area Number |
23658016
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Crop science/Weed science
|
Research Institution | Nagoya University |
Principal Investigator |
|
Research Collaborator |
MIYAKE Hiroshi 名古屋大学, 大学院・生命農学研究科, 教授 (60134798)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 葉緑体 / 窒素同化 / 環境ストレス / 輸送体 / 還元力 / リンゴ酸バルブ / シロイヌナズナ / 光阻害 / 植物 / 遺伝子組換え |
Research Abstract |
Export of reducing equivalents from chloroplasts is necessary to prevent photoinhibition and to supply reducing power to cytosolic nitrate reductase. The transport process is mediated by malate valve comprising 2-oxoglutarate/malate transporter (OMT) and malate dehydrogenase (MDH) isozymes. We produced Arabidopsistransformants overexpressing OMT and stromal NADP-MDH, and found that the tolerance to environmental stresses and the ability of nitrate assimilation are improved in the transformants. Therefore, malate valve would be a new target to improve environmental tolerance and nitrogen metabolism of crops.
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