An attempt to improve nitrogen metabolism and environmental tolerance of crops by enhancement of the ability of malate valve

• Project/Area Number: 23658016
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Crop science/Weed science
• Research Institution: Nagoya University
• Principal Investigator: TANIGUCHI Mitsutaka 名古屋大学, 生命農学研究科, 准教授 (40231419)
• Research Collaborator: MIYAKE Hiroshi 名古屋大学, 大学院・生命農学研究科, 教授 (60134798)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 葉緑体 / 窒素同化 / 環境ストレス / 輸送体 / 還元力 / リンゴ酸バルブ / シロイヌナズナ / 光阻害 / 植物 / 遺伝子組換え

Research Abstract

Export of reducing equivalents from chloroplasts is necessary to prevent photoinhibition and to supply reducing power to cytosolic nitrate reductase. The transport process is mediated by malate valve comprising 2-oxoglutarate/malate transporter (OMT) and malate dehydrogenase (MDH) isozymes. We produced Arabidopsistransformants overexpressing OMT and stromal NADP-MDH, and found that the tolerance to environmental stresses and the ability of nitrate assimilation are improved in the transformants. Therefore, malate valve would be a new target to improve environmental tolerance and nitrogen metabolism of crops.

Research Products

• [Journal Article] Localization of reactive oxygen species and change of antioxidant capacities in mesophyll and bundle sheath chloroplasts of maize under salinity (2013)
  • Author(s): Omoto, E., Nagao, H., Taniguchi, M. and Miyake, H
  • Journal Title: Physiologia Plantarum
  • Peer Reviewed
• [Journal Article] Redox-shuttling between chloroplast and cytosol : integration of intra-and extra-chloroplast metabolism (2012)
  • Author(s): Taniguchi, M. and Miyake, H.
  • Journal Title: Current Opinion in Plant Biology Volume: (印刷中) Issue: 3 Pages: 252-260
  • DOI: 10.1016/j.pbi.2012.01.014
  • Peer Reviewed
• [Journal Article] 植物のCO2固定機能を高めるC4光合成の最近の話題 (2012)
  • Author(s): 谷口光隆
  • Journal Title: 日本の科学者 Volume: 47 Pages: 307-311
  • URL: http://www.jsa.gr.jp/04pub/index.html#nihonnokagakusya
  • Peer Reviewed
• [Journal Article] 植物のCＯ2固定機能を高めるＣ4光合成の最近の話題 (2012)
  • Author(s): 谷口光隆
  • Journal Title: 日本の科学者 Volume: 47 Pages: 307-311
  • Peer Reviewed
• [Journal Article] The avoidance and aggregative movements of mesophyll chloroplasts in C4 monocots in response to blue light and abscisic acid (2011)
  • Author(s): Maai, E., Shimada, S., Yamada, M., Sugiyama, T., Miyake, H. and Taniguchi, M.
  • Journal Title: Journal of Experimental Botany Volume: 62 Issue: 9 Pages: 3213-3221
  • DOI: 10.1093/jxb/err008
  • Peer Reviewed
• [Journal Article] Differntial positioning of chloroplasts in C4 mesophyll and bundle sheath cells (2011)
  • Author(s): Maai, E., Miyake, H. and Taniguchi, M.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Issue: 8 Pages: 1111-1113
  • DOI: 10.4161/psb.6.8.15809
• [Journal Article] 青色光とABAに応答したC4植物の葉肉細胞における葉緑体の運動 (2011)
  • Author(s): 間合絵里,三宅博,谷口光隆
  • Journal Title: 光合成研究 Volume: 21 Pages: 16-19
  • NAID: 40018857351
  • URL: http://photosyn.c.u-tokyo.ac.jp/
  • Peer Reviewed
• [Journal Article] The chloroplastic 2-oxoglutarate/malate transporter has dual function as the malate valve and in carbon/nitrogen metabolism (2011)
  • Author(s): Kinoshita, H., Nagasaki, J., Yoshikawa, N., Yamamoto, A., Takito, S., Kawasaki, M., Sugiyama, T., Miyake, H., Weber, A. P. M. and Taniguchi, M.
  • Journal Title: Plant Journal Volume: 65 Issue: 1 Pages: 15-26
  • DOI: 10.1111/j.1365-313x.2010.04397.x
  • Peer Reviewed
• [Presentation] 葉肉葉緑体凝集運動の C_4植物における普遍性とその生理的意義 (2013)
  • Author(s): 塚口駿貴,大井崇生,三宅博,谷口光隆
  • Organizer: 日本作物学会第235回講演会
  • Place of Presentation: 川崎
• [Presentation] ABAと青色光に応答した葉肉葉緑体の凝集運動はC4植物に共通した生理応答である (2012)
  • Author(s): 塚口駿貴,間合絵里,島田祥宇,大井崇生,三宅博,谷口光隆
  • Organizer: 日本作物学会第234回講演会
  • Place of Presentation: 仙台
• [Presentation] 葉肉葉緑体の凝集運動はC4植物に共通した生理応答である (2012)
  • Author(s): 谷口光隆,塚口駿貴,間合絵里,島田祥宇,三宅博
  • Organizer: 第3回日本光合成学会
  • Place of Presentation: 横浜
• [Presentation] Movement of chloroplasts in C4mesophyll cells in response to blue light and abscisic acid (2011)
  • Author(s): Maai, E., Miyake, H. and Taniguchi, M
  • Organizer: The 7th Asian Crop Science Association Conference
  • Place of Presentation: Bogor, Indonesia
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~shigen/