| Project/Area Number |
23658017
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop science/Weed science
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| Research Institution | Kyoto University |
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Principal Investigator |
INOUE Hiromo 京都大学, 大学院・農学研究科, 講師 (40260616)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 栽培体系 / 生物的窒素固定 / アセチレン還元活性 / 重窒素追跡 |
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| Research Abstract |
Rice cultivar Taichung 65, Nipponbare, Kasalath and C5444 were cultivated in the pots using masa soil. By using 15N labeled (NH4)2SO4 as nitrogen fertilizer and pure water as irrigation through total rice growth term, the amount of biological nitrogen fixation in rice could be estimated in the pot experiment. According to this method, biological nitrogen fixation in the F2 population from the cross between Taichung 65 and C5444 was estimated and it was revealed that this agronomic character may be controlled by genetic factors. Nitrogen fixation microorganisms could be easily detected using the PCR with DNA extracted from the soils, with which rice was cultivated, as the template DNA.
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