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Direct injection of florigen to reduce the juvenile phase of Citrus species

Research Project

Project/Area Number 23658031
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Horticulture/Landscape architecture
Research InstitutionSaga University

Principal Investigator

NAGANO YUKIO  佐賀大学, 総合分析実験センター, 准教授 (00263038)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Ryoji  佐賀大学, 総合分析実験センター, 客員研究員 (40355409)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords園芸学 / バイオテクノロジー / 発生・分化 / 蛋白質 / 植物
Research Abstract

Control of Flowering is a dream of farmers. Especially in fruit trees, juvenile phase (the time between sowing and first flowering) is generally long. In the case of Citrus species, this period is more than 8 years. Therefore, to shorten this period is important for the cultivation of these trees. FLOWERING LOCUS T (FT) protein is the most potent candidate of flowering hormone "florigen". To reduce the juvenile phase of Citrus species, we directly injected bacterially-expressed FT protein into these plants. Although we sometimes seceeded to induce the flowering, we did not always do it. Alternatively, we searched the chemical inhibitors against the flowering inhibitor TFL1 protein. However, these attempts were failed

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (47 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (33 results) (of which Invited: 1 results) Remarks (3 results) Patent(Industrial Property Rights) (8 results) (of which Overseas: 6 results)

  • [Journal Article] Characterization of limes (Citrus aurantifolia) grown in Bhutan and Indonesia using high-throughput sequencing2014

    • Author(s)
      Tshering Penjor, Takashi Mimura, Ryoji Matsumoto, Masashi Yamamoto, Yukio Nagano
    • Journal Title

      Sci. Rep

      Volume: 4 Issue: 1 Pages: 4853-4853

    • DOI

      10.1038/srep04853

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultra-Low Background DNA Cloning System2013

    • Author(s)
      Kenta Goto, Yukio Nagano
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 2 Pages: e56530-e56530

    • DOI

      10.1371/journal.pone.0056530

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phylogenetic Relationships of Citrus and Its Relatives Based on matK Gene Sequences.2013

    • Author(s)
      Tshering Penjor, Masashi Yamamoto, Miki Uehara, Manami Ide, Natsumi Matsumoto, Ryoji Matsumoto, Yukio Nagano
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 4 Pages: e62574-e62574

    • DOI

      10.1371/journal.pone.0062574

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウンシュウミカンのトランスクリプトーム解析2014

    • Author(s)
      永野 幸生, 三村 高史, 松本 亮司
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学農学部
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Genomics and Transcriptomics of Citrus species2013

    • Author(s)
      Yukio Nagano
    • Organizer
      The 8th Joint Seminar between Daegu University and Saga University
    • Place of Presentation
      大韓民国・大邱大学校
    • Related Report
      2013 Final Research Report
  • [Presentation] Ultra-Low Background DNA Cloning System2013

    • Author(s)
      Kenta Goto, Yukio Nagano
    • Organizer
      The 8th Joint Seminar between Daegu University and Saga University
    • Place of Presentation
      大韓民国・大邱大学校
    • Related Report
      2013 Final Research Report
  • [Presentation] 奇怪な植物を発見!2013

    • Author(s)
      永野幸生, Tshering Penjor, 三村高史, 松本亮司, 山本雅史
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      ゆやど雲仙新湯(長崎県雲仙市)
    • Related Report
      2013 Final Research Report
  • [Presentation] "Ultra-Low Background DNA Cloning System" THE FINAL2013

    • Author(s)
      後藤健太, 永野幸生
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      ゆやど雲仙新湯(長崎県雲仙市)
    • Related Report
      2013 Final Research Report
  • [Presentation] ブータン王国のライムのRAD-seq 解析2013

    • Author(s)
      永野幸生, Tshering Penjor, 三村高史, 松本亮司, 山本雅史
    • Organizer
      NGS現場の会 第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] ウンシュウミカンのゲノム解析とRNAシーケンシング解析2013

    • Author(s)
      三村高史, 松本亮司, 永野幸生
    • Organizer
      NGS 現場の会第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] ライムの分子歴史地理学2013

    • Author(s)
      永野幸生, Tshering Penjor, 三村高史, 松本亮司, 山本雅史
    • Organizer
      平成25年度日本生化学会九州支部例会
    • Place of Presentation
      佐賀大学農学部
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] バックグラウンドがゼロのDNA クローニング法2013

    • Author(s)
      後藤健太, 永野幸生
    • Organizer
      平成25年度日本農芸化学会大会
    • Place of Presentation
      宮城
    • Related Report
      2013 Final Research Report
  • [Presentation] ウンシュウミカンのゲノム解析とRNAシーケンシング解析2013

    • Author(s)
      三村高史、松本亮司、永野幸生
    • Organizer
      NGS現場の会 第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] ブータン王国のライムのRAD-seq解析2013

    • Author(s)
      永野幸生、Tshering Penjor、三村高史、松本亮司、山本雅史
    • Organizer
      NGS現場の会 第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] “Ultra-Low Background DNA Cloning System” THE FINAL2013

    • Author(s)
      後藤健太、永野幸生
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      ゆやど雲仙新湯(長崎県雲仙市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 奇怪な植物を発見!2013

    • Author(s)
      永野幸生、Tshering Penjor、三村高史、松本亮司、山本雅史
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      ゆやど雲仙新湯(長崎県雲仙市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ultra-Low Background DNA Cloning System2013

    • Author(s)
      Kenta Goto, Yukio Nagano
    • Organizer
      The 8th Joint Seminar between Daegu University and Saga University
    • Place of Presentation
      大邱大学校(大韓民国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Genomics and Transcriptomics of Citrus species2013

    • Author(s)
      Yukio Nagano
    • Organizer
      The 8th Joint Seminar between Daegu University and Saga University
    • Place of Presentation
      大邱大学校(大韓民国)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] バックグラウンドがゼロのDNAクローニング法2013

    • Author(s)
      後藤健太、永野幸生
    • Organizer
      平成25年度日本農芸化学会大会
    • Place of Presentation
      宮城
    • Related Report
      2012 Research-status Report
  • [Presentation] Whole genome and transcriptome sequencing in Citrus leaves to search floral genes2012

    • Author(s)
      Takashi Mimura, Hiroaki Suzuki, Ryoji Matsumoto, Yukio Nagano
    • Organizer
      The 7th Saga University-Daegu University Joint Seminar
    • Place of Presentation
      佐賀
    • Year and Date
      2012-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Zero Background Cloning System2012

    • Author(s)
      Kenta Goto, Yukio Nagano
    • Organizer
      The 7th Saga University-Daegu University Joint Seminar
    • Place of Presentation
      佐賀
    • Year and Date
      2012-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] RNA シーケンシングによるカンキツ遺伝子の網羅的発現解析および開花関連遺伝子の探索2012

    • Author(s)
      三村高史, 鈴木裕明, 松本亮司, 永野幸生
    • Organizer
      九州育種談話会
    • Place of Presentation
      福岡
    • Year and Date
      2012-10-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Zero Background Cloning System2012

    • Author(s)
      後藤健太, 永野幸生
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 組換えプラスミド構築において偽陽性発生を極限まで低下させる技術の開発2012

    • Author(s)
      後藤健太, 永野幸生
    • Organizer
      平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Final Research Report
  • [Presentation] カンキツにおける開花関連遺伝子の探索および機能解析2012

    • Author(s)
      三村高史, 鈴木裕明, 松本亮司, 永野幸生
    • Organizer
      第36回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      宮崎
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 多重宿主組換え法 : 組換えDNA 構築において偽陽性発生を極限まで低下させる方法2012

    • Author(s)
      後藤健太, 永野幸生
    • Organizer
      第36回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      宮崎
    • Related Report
      2013 Final Research Report
  • [Presentation] カンキツの開花制御に関わるタンパク質間の相互作用2012

    • Author(s)
      三村高史, 鈴木裕明, 松本亮司, 永野幸生
    • Organizer
      第30回日本植物分子細胞生物学会
    • Place of Presentation
      奈良
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Zero Background Cloning System2012

    • Author(s)
      後藤健太, 永野幸生
    • Organizer
      平成24年日本生化学会九州支部例会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 多重宿主組換え法:組換えDNA構築において偽陽性発生を極限まで低下させる方法2012

    • Author(s)
      後藤健太、永野幸生
    • Organizer
      第36回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      宮崎
    • Related Report
      2012 Research-status Report
  • [Presentation] 「組換えプラスミド構築において偽陽性発生を極限まで低下させる技術の開発2012

    • Author(s)
      後藤健太、永野幸生
    • Organizer
      平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Research-status Report
  • [Presentation] RNAシーケンシングによるカンキツ遺伝子の網羅的発現解析および開花関連遺伝子の探索2012

    • Author(s)
      三村高史、鈴木裕明、松本亮司、 永野幸生
    • Organizer
      九州育種談話会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Zero Background Cloning System2012

    • Author(s)
      Kenta Goto, Yukio Nagano
    • Organizer
      The 7th Saga University–Daegu University Joint Seminar
    • Place of Presentation
      佐賀
    • Related Report
      2012 Research-status Report
  • [Presentation] Whole genome and transcriptome sequencing in Citrus leaves to search floral genes2012

    • Author(s)
      Takashi Mimura, Hiroaki Suzuki, Ryoji Matsumoto and Yukio Nagano
    • Organizer
      The 7th Saga University–Daegu University Joint Seminar
    • Place of Presentation
      佐賀
    • Related Report
      2012 Research-status Report
  • [Presentation] Comparison of the model of flowering regulation systems between Citrus and Arabidopsis2011

    • Author(s)
      Takashi Mimura, Hiroaki Suzuki, Ryoji Matsumoto, Yukio Nagano
    • Organizer
      The 6th Daegu University-Saga University Joint Symposium
    • Place of Presentation
      大韓民国・大邱大学校
    • Year and Date
      2011-11-01
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] カンキツとシロイヌナズナの開花制御モデルの比較2011

    • Author(s)
      三村高史, 鈴木裕明, 松本亮司, 永野幸生
    • Organizer
      平成23年度日本農芸化学会西日本支部・中四国支部合同大会
    • Place of Presentation
      宮崎大学
    • Year and Date
      2011-09-17
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] カンキツにおける開花制御モデルの検討2011

    • Author(s)
      三村高史, 鈴木裕明, 松本亮司, 永野幸生
    • Organizer
      平成23年度日本生化学会九州支部例会
    • Place of Presentation
      久留米大学
    • Year and Date
      2011-05-22
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Remarks]

    • URL

      http://www.iac.saga-u.ac.jp/lifescience/

    • Related Report
      2013 Final Research Report
  • [Remarks] 総合分析実験センター 生物資源開発部門(本庄地区)

    • URL

      http://www.iac.saga-u.ac.jp/lifescience/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 総合分析実験センター 生物資源開発部門(本庄地区)

    • URL

      http://www.iac.saga-u.ac.jp/lifescience/

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生, 松本亮司
    • Industrial Property Rights Holder
      永野幸生, 松本亮司
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2011-540549
    • Filing Date
      2012-05-08
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生, 松本亮司
    • Industrial Property Rights Holder
      永野幸生, 松本亮司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-05-25
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生, 松本亮司
    • Industrial Property Rights Holder
      永野幸生, 松本亮司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-05-25
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生, 松本亮司
    • Industrial Property Rights Holder
      永野幸生, 松本亮司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-05-25
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生、松本亮司
    • Industrial Property Rights Holder
      永野幸生、松本亮司
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2011-540549
    • Filing Date
      2012-05-08
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生、松本亮司
    • Industrial Property Rights Holder
      永野幸生、松本亮司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-05-25
    • Related Report
      2012 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生、松本亮司
    • Industrial Property Rights Holder
      永野幸生、松本亮司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-05-25
    • Related Report
      2012 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 開花誘導剤2012

    • Inventor(s)
      永野幸生、松本亮司
    • Industrial Property Rights Holder
      永野幸生、松本亮司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-07-31
    • Related Report
      2012 Research-status Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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