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Development of novel rice blast control system, which suppresses mutations in the pathogen

Research Project

Project/Area Number 23658038
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant pathology
Research InstitutionHokkaido University

Principal Investigator

SONE Teruo  北海道大学, (連合)農学研究科(研究院), 准教授 (00333633)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsイネ / いもち病 / DNA修復 / 複合体 / 突然変異 / スクリーニング / いもち病菌 / DNA組換え修復 / 阻害剤 / ポジティブスクリーニング / バイオテクノロジー / 菌類 / 組換え修復
Research Abstract

Rice blast is the most important disease of rice. One of the big problem in rice blast control is the mutation of pathogen that enables pathogens to overcome rice blast resistance. Mutations in rice blast fungus is mostly occur through DNA recombinations. This study aimed to develop a novel rice blast control strategy, which suppresses mutations in the pathogens, by studying the DNA recombination proteins in the pathogen. Results of this study indicated that DNA recombination is very important for the pathogen to cause the disease. DNA recombination proteins works as a complex will be a target for controlling the disease and suppressing the mutations. In this study, we also developed a screening system for inhibitors for protein-protein interaction.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (14 results) (of which Invited: 3 results)

  • [Journal Article] Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae2013

    • Author(s)
      Teruo Sone, Saori Takeuchi, Shinsuke Miki, Yuki Satoh, Keisuke Ohtsuka, Ayumi Abe, and Kozo Asano
    • Journal Title

      FEMS Microbiology Letters

      Volume: 339 Pages: 102-109

    • Related Report
      2013 Final Research Report
  • [Journal Article] Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae2013

    • Author(s)
      Teruo Sone, Saori Takeuchi, Shinsuke Miki, Yuki Satoh, Keisuke Ohtsuka, Ayumi Abe, and Kozo Asano
    • Journal Title

      FEMS Mivrobiology Letters

      Volume: 339 Issue: 2 Pages: 102-109

    • DOI

      10.1111/1574-6968.12058

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] イネいもち病菌のエフェクターと宿主R遺伝子の微妙な関係2011

    • Author(s)
      曾根輝雄
    • Journal Title

      化学と生物

      Volume: 49 Pages: 292-294

    • Related Report
      2011 Research-status Report
  • [Presentation] イネいもち病菌のAVR-Pik の変異機構の解析2014

    • Author(s)
      船引麻衣, 竹内紗央里, 中馬いづみ, 浅野行蔵, 曾根輝雄
    • Organizer
      日本植物病理学会平成26年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2014-06-03
    • Related Report
      2013 Final Research Report
  • [Presentation] イネいもち病防除法の開発に向けたタンパク質間相互作用阻害物質のスクリーニング系の構築2014

    • Author(s)
      中山 航, 阿部 歩, 浅野行蔵, 曾根輝雄
    • Organizer
      日本植物病理学会平成26年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2014-06-03
    • Related Report
      2013 Final Research Report
  • [Presentation] イネいもち病菌のDNA 修復遺伝子Mhm11, Rhm50, Nhm1 の解析2014

    • Author(s)
      阿部 歩, 浅野行蔵, 曾根輝雄
    • Organizer
      日本植物病理学会平成26年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2014-06-03
    • Related Report
      2013 Final Research Report
  • [Presentation] イネいもち病菌のDNA組換え修復遺伝子Mhm11, Rhm50, Nhm1の解析2014

    • Author(s)
      阿部 歩,浅野行蔵,曾根輝雄
    • Organizer
      日本植物病理学会平成26年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネいもち病防除法の開発に向けたタンパク質間相互作用阻害物質のスクリーニング系の構築2014

    • Author(s)
      中山 航,阿部 歩,浅野行蔵,曾根輝雄
    • Organizer
      日本植物病理学会平成26年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネいもち病菌のAVR-Pikの変異機構の解析2014

    • Author(s)
      船引麻衣,竹内紗央里,中馬いづみ,浅野行蔵,曾根輝雄
    • Organizer
      日本植物病理学会平成26年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネいもち病菌の病原性変異に関する分子遺伝学的研究2013

    • Author(s)
      曾根輝雄
    • Organizer
      日本農芸化学会北海道支部講演会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2013-11-30
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Study on mutation mechanism of AVR-Pia and AVR-Pik in Magnaporthe oryzae2013

    • Author(s)
      Funabiki M., Takeuchi S., Satoh Y., and Sone, T
    • Organizer
      International Rice Blast Conference
    • Place of Presentation
      Ramada Plaza Jeju Hotel, Jejudo, South Korea
    • Year and Date
      2013-08-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Study on mutation mechanism of AVR-Pia and AVR-Pik in Magnaporthe oryzae2013

    • Author(s)
      Funabiki M, Takeuchi S, Sato Y, and Sone T.
    • Organizer
      International Rice Blast Conference
    • Place of Presentation
      Ramada Plaza Hotel, Jejudo, South Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] Strategy of the rice blast fungus to deal with blast resistant cultivars of rice2012

    • Author(s)
      Teruo Sone
    • Organizer
      International Conference on Biosciences and Biotechnology
    • Place of Presentation
      Udayana University, Bali (Indonesia)
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] イネいもち病菌のDNA 損傷シグナルトランスデューサーp53BP1 の解析2012

    • Author(s)
      田鹿 結, 阿部 歩, 曾根輝雄
    • Organizer
      日本農芸化学会北海道支部夏期シンポジウム
    • Place of Presentation
      北海道大学水産学部講堂(函館市)
    • Year and Date
      2012-07-27
    • Related Report
      2013 Final Research Report
  • [Presentation] イネいもち病菌のDNA 損傷シグナルトランスデューサーp53BP1 の解析2012

    • Author(s)
      田鹿 結、阿部 歩、曾根輝雄
    • Organizer
      日本農芸化学会北海道支部夏期シンポジウム
    • Place of Presentation
      北海道大学水産学部講堂(函館)
    • Related Report
      2012 Research-status Report
  • [Presentation] Strategy of the rice blast fungus to deal with blast resistant cultivars of rice2012

    • Author(s)
      Teruo Sone
    • Organizer
      International conference on biosciences and biotechnology, Bali
    • Place of Presentation
      Udayana University, Bali,Indonesia
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] イネいもち病菌の病原性変異に関する研究

    • Author(s)
      曾根輝雄
    • Organizer
      日本農芸化学会北海道支部講演会
    • Place of Presentation
      北海道大学農学部(札幌市)
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2011-08-05   Modified: 2019-07-29  

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