Identification of intracellular degradation systems required for infection strategies of phytopathogenic fungus and its application

• Project/Area Number: 23658040
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant pathology
• Research Institution: Kyoto University
• Principal Investigator: TAKANO YOSHITAKA 京都大学, (連合)農学研究科(研究院), 准教授 (80293918)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 植物病原糸状菌 / 病原性 / 細胞内分解システム / オートファジー / ペルオキシソーム / RNA / Pボディ / 炭疽病菌

Research Abstract

Eukaryotic organisms commonly possess various cellular degradation systems, suggesting the importance of 'degradation'. However, there is limited information about roles of cellular degradation system for host infection strategies of phytopathogenic fungi. In this study, we aimed to reveal outlines of cellular degradation systems involved in the pathogens' infection strategies. As a result, we revealed that ATG11 is required for pathogenicity of anthracnose fungus Colletotrichum orbiculare, and also is involved in selective autophagic degradation of peroxisomes. We also suggested that non-selective autophagic degradation is required for pathogenicity of the pathogen. Furthermore, we have generated a set of transgenic C. orbiculare reporter lines to monitor the status of each degradation system, including the dynamics of P bodies involved in mRNA degradation, which will be applied for screening of chemicals that inhibit corresponding degradation systems of the pathogen.

Research Products

• [Journal Article] The emerging role of autophagy in peroxisome dynamics and lipid metabolism of phyllosphere microorganisms (2014)
  • Author(s): Masahide Oku, Yoshitaka Takano, Yasuyoshi Sakai
  • Journal Title: Frontiers in Plant Science Volume: Vol.5 Pages: 81-81
  • DOI: 10.3389/fpls.2014.00081
  • NAID: 120005439549
  • Peer Reviewed
• [Journal Article] Dynamics of infection-related morphogenesis and pathogenesis in Colletotrichum orbiculare (2013)
  • Author(s): Yasuyuki Kubo, and Yoshitaka Takano
  • Journal Title: Journal of General Plant Pathology Volume: Vol.79 Issue: 4 Pages: 233-242
  • DOI: 10.1007/s10327-013-0451-9
  • NAID: 10031184146
  • Peer Reviewed
• [Journal Article] Primary and secondary metabolism regulates lipolysis in appressoria of Colletotrichum orbiculare (2012)
  • Author(s): M. Asakura, K. Yoshino, A.M.. Hill, Y. Kubo, Y. Sakai, and Y. Takano.
  • Journal Title: Fungal Genet. Biol Volume: 49 Issue: 11 Pages: 967-97
  • DOI: 10.1016/j.fgb.2012.08.009
  • Peer Reviewed
• [Journal Article] LAC2 encoding a secreted laccase is involved in appressorial melanization and conidial pigmentation in Colletotrichum orbiculare (2012)
  • Author(s): Lin, S.Y., Okuda, S., Ikeda, K., Okuno, T. and Takano, Y.
  • Journal Title: Molecular and Plant-Microbe Interactions Volume: 25 Issue: 12 Pages: 1552-1561
  • DOI: 10.1094/mpmi-05-12-0131-r
  • Peer Reviewed
• [Presentation] The relation of dicer-dependent RNA interference with infection-related development in a plant pathogenic fungus Colletotrichum orbiculare (2014)
  • Author(s): Suthitar Singkaravanit-Ogawa, Pamela Gan, Ken Shirasu, and Yoshitaka Takano
  • Organizer: 12 th European Conference on Fungal Genetics
  • Place of Presentation: Seville, Spain
  • Year and Date: 2014-03-23
• [Presentation] 植物と病原菌の戦い (2013)
  • Author(s): 高野義孝
  • Organizer: 日本微生物学連盟フォーラム「驚きの微生物たち」
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2013-01-26
• [Presentation] 転写後制御系遺伝子AGO1 とCSX1は, ウリ類炭疽病菌の病原性関連遺伝子である (2012)
  • Author(s): 劉毅, 高橋史香, 奥野哲郎, 高野義孝
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-03-28
• [Presentation] ウリ類炭疽病菌の病原性に必須である分泌タンパク質遺伝子LAC2 の同定及び機能解析 (2011)
  • Author(s): 林紹伃, 奥田枝穂, 奥野哲郎, 高野義孝
  • Organizer: 糸状菌分子生物学カンファレンス
  • Place of Presentation: 東京大学弥生講堂・一条ホール/アネックス
  • Year and Date: 2011-11-17
• [Presentation] ウリ類炭疽病菌の病原性に必須である分泌タンパク質遺伝子LAC2の同定及び機能解析 (2011)
  • Author(s): 林紹伃, 奥田枝穂, 奥野哲郎, 高野義孝
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: サンポートホール高松
  • Year and Date: 2011-10-01
• [Presentation] 植物病原糸状菌の侵入戦略とオートファジー (2011)
  • Author(s): 高野義孝
  • Organizer: 糸状菌遺伝子研究会
  • Place of Presentation: 北トピア
  • Year and Date: 2011-06-03
• [Presentation] 植物病原糸状菌の侵入戦略とオートファジー (2011)
  • Author(s): 高野義孝
  • Organizer: 糸状菌遺伝子研究会(招待講演)
  • Place of Presentation: 北トピア（東京都）