Molecular mechanisms of pathogenicity of phytopathogenic and entomopathogenic fungi based on a common toxin production

• Project/Area Number: 23658041
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant pathology
• Research Institution: Tottori University
• Principal Investigator: KODAMA Motoichiro 鳥取大学, 農学部, 教授 (00183343)
• Co-Investigator(Renkei-kenkyūsha): NAKA Hideshi 鳥取大学, 農学部, 助教 (00443846)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物病原菌 / 昆虫病原菌 / 毒素 / 病原性 / 毒素生合成遺伝子

Research Abstract

A phytopathogen Alternaria and an entomopathogen Metarhizium produce the common toxin destruxin and infect plant and insect hosts, respectively. On the other hand, a phytopathogen Fusarium and an entomopathogen Beauveria produce the common toxin boubericin. Both toxins are cyclic peptides. To elucidate pathological roles of those common toxins produced by both phytopathogenic and entomopathogenic fungi, cloning and characterization of genes for the toxin biosynthesis in those pathogens have been tried in this study.

Research Products

• [Journal Article] Alternaria属菌の病原性進化 (2013)
  • Author(s): 赤木靖典、柘植尚志、児玉基一朗
  • Journal Title: 植物防疫 Volume: 67巻3号 Pages: 15-19
  • NAID: 40019614997
  • Peer Reviewed
• [Journal Article] Identification of the sex pheromone secreted by Synanthedon tenuis(Lepidoptera: Sesiidae). (2013)
  • Author(s): Naka, H, Suzuki, T., Watarai, T., Horie, Y.,Mochizuki, F., Mochizuki, A.,Tsuchida, K., Arita, Y., Ando, T.
  • Journal Title: Applied Entomology and Zoology Volume: 48(1) Pages: 27-33
  • NAID: 40019588058
  • Peer Reviewed
• [Journal Article] Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata (2013)
  • Author(s): Takashi Tsuge
  • Journal Title: FEMS Microbiology Reviews Volume: 37 Issue: 1 Pages: 44-66
  • DOI: 10.1111/j.1574-6976.2012.00350.x
  • Peer Reviewed
• [Journal Article] Alternaria属菌の病原性進化 (2013)
  • Author(s): 赤木靖典
  • Journal Title: 植物防疫 Volume: 67 Pages: 15-19
  • Peer Reviewed
• [Journal Article] Functional analysis of the ceramide synthase gene ALT7, a homolog of the disease resistance gene Asc1, in the plant pathogen Alternaria alternata (2012)
  • Author(s): Kheder, A. A., Akagi, Y., Tsuge, T., and Kodama, M
  • Journal Title: J. Plant Pathol. Microbiol. Volume: S2 Issue: S2
  • DOI: 10.4172/2157-7471.s2-001
  • Peer Reviewed
• [Journal Article] Functional analysis of the melanin biosynthesis genes ALM1 and BRM2-1 in the tomato pathotype of Alternaria alternata (2012)
  • Author(s): Kheder AA, Akagi Y, Yanaga K, Maekawa N, Otani H, Tsuge T, Kodama M
  • Journal Title: J Gen Plant Pathol Volume: 78 Issue: 1 Pages: 30-38
  • DOI: 10.1007/s10327-011-0356-4
  • NAID: 10030561778
  • Peer Reviewed
• [Presentation] Alternaria alternata病原菌の宿主特異的毒素生産におけるglobal regulator LaeAホモログの関与 (2013)
  • Author(s): 高尾和実、赤木靖典、播本佳明、柘植尚志、難波栄二、児玉基一朗
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2013-03-28
• [Presentation] LaeA regulate secondary metabolite in multi pathotypes of the fungal plant pathogen Alternaria alternata (2012)
  • Author(s): Takao, K., Akagi, Takashi, Y., Tsuge, T.and Kodama, M.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-13
• [Presentation] 植物病原菌Alternaria alternataが保有する病原性染色体の構造と機能 (2012)
  • Author(s): 赤木靖典、播本佳明、柘植尚志、児玉基一朗
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-13
• [Presentation] トマトアルターナリア茎枯病菌が保有する病原染色体の構造と機能 (2012)
  • Author(s): 赤木靖典、播本佳明、柘植尚志、尾谷 浩、児玉基一朗
  • Organizer: 平成24年度日本植物病理学会関西部会
  • Place of Presentation: 鳥取市
  • Year and Date: 2012-09-28
• [Presentation] Alternaria alternata病原菌におけるglobal regulator LaeAホモログの機能解析 (2012)
  • Author(s): 高尾和実、赤木靖典、播本佳明、柘植尚志、尾谷 浩、児玉基一朗
  • Organizer: 平成24 年度日本植物病理学会関西部会
  • Place of Presentation: 鳥取市
  • Year and Date: 2012-09-28
• [Presentation] 植物病原糸状菌における二次代謝産物生合成と病原性 -ゲノム解析からみた進化・多様性-. 植物-病原微生物の相互作用研究の新展開 (2012)
  • Author(s): 児玉基一朗、赤木靖典、高尾和実、柘植尚志
  • Organizer: 平成24 年度日本植物病理学会感染生理談話会
  • Place of Presentation: 近江八幡市
  • Year and Date: 2012-09-01
• [Presentation] Structure of a conditionally dispensable pathogenicity chromosome of the tomato pathotype of Alternaria alternata. (2012)
  • Author(s): Akagi, Y., Tsuge, T. and Kodama, M
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2012-08-01
• [Presentation] Structural analysis of a conditionally dispensable chromosome of the tomato pathotype of Alternaria alternata. (2012)
  • Author(s): Akagi, Y., Harimoto, Y., Tsuge, T. and Kodama,M
  • Organizer: 2nd Japan-Korea Joint Symposium on Plant Pathology
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2012-03-27
• [Presentation] 植物病原性Alternariaにおける病原性の進化と分化 -遺伝子水平移動が関与か？- (2012)
  • Author(s): 赤木靖典
  • Organizer: 日本植物病理学植物病原菌類談話会(招待講演)
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] Pathogenicity chromosomes in host-specific toxin-producing Alternaria species. (2011)
  • Author(s): Akagi, Y., Takao, K., Harimoto, Y., Tsuge, T.and Kodama, M.
  • Organizer: IUMS (International Union of Mycrobiological Societies) 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-08
• [Presentation] Functional analysis of a global regulator, LaeA in the fungal plant pathogen Alternaria alternata. (2011)
  • Author(s): Takao, K., Akagi, Y., Tsuge, T. and Kodama,M.
  • Organizer: Asian Mycological Congress 2011
  • Place of Presentation: Incheon, Korea
  • Year and Date: 2011-08-09
• [Presentation] The structure of the conditionally dispensable chromosome of Alternaria alternata tomato pathotype (2011)
  • Author(s): Akagi, Y.
  • Organizer: Asian Mycology Congress 2011
  • Place of Presentation: Incheon, Korea
• [Presentation] Functional analysis of a global regulator, LaeA in the fungal plant pathogen Alternaria alternata (2011)
  • Author(s): Takao, K.
  • Organizer: Asian Mycology Congress 2011
  • Place of Presentation: Incheon, Korea
• [Presentation] トマトアルターナリア茎枯病菌が保有するconditionally dispensable chromosome (CDC) の構造解析 (2011)
  • Author(s): 赤木靖典
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: （サンポートホール高松）高松市
• [Presentation] Structure of a conditionally dispensable pathogenicity chromosome of the tomato pathotype of Alternaria alternata
  • Author(s): Akagi, Y.
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto, Japan
• [Presentation] 植物病原糸状菌における二次代謝産物生合成と病原性 ―ゲノム解析からみた進化・多様性―
  • Author(s): 児玉基一朗
  • Organizer: 平成24年度日本植物病理学会感染生理談話会
  • Place of Presentation: 近江八幡市
  • Invited
• [Presentation] Alternaria alternata病原菌におけるglobal regulator LaeAホモログの機能解析
  • Author(s): 高尾和実
  • Organizer: 平成24年度日本植物病理学会関西部会
  • Place of Presentation: 鳥取市
• [Presentation] LaeA regulate secondary metabolite in multi pathotypes of the fungal plant pathogen Alternaria alternata
  • Author(s): Takao, K.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] Alternaria alternata病原菌の宿主特異的毒素生産におけるglobal regulator LaeAホモログの関与
  • Author(s): 高尾和実
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜市
• [Book] The Mycota, Karl Esser et al. eds. Springer-Ver la g , Berlin Heidelberg New York (2013)
  • Author(s): Johnson,R.D., Akagi,Y., Fleetwood, D.J.Gardiner, D.M., Kodama, M., Young, C.A.,Vo is e y, C.R.
  • Publisher: Fungal toxins of agricultural importance.
• [Book] The Mycota (2013)
  • Author(s): Johnson,R.D.
  • Publisher: Springer-Verlag
• [Book] 植物病原糸状菌における二次代謝産物生合成と病原性 -ゲノム解析からみた進化・多様性-.植物-病原微生物の相互作用研究の新展開 (2012)
  • Author(s): 児玉基一朗、赤木靖典、高尾和実、柘植尚志
  • Publisher: 日本植物病理学会
• [Book] 植物－病原微生物の相互作用研究の新展開 (2012)
  • Author(s): 児玉基一朗
  • Total Pages: 155
  • Publisher: 日本植物病理学会