| Project/Area Number |
23658042
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
MIYOSHI Hiroshi 聖マリアンナ医科大学, 医学部, 講師 (80322519)
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| Co-Investigator(Kenkyū-buntansha) |
TOMOO Koji 大阪薬科大学, 薬学部, 准教授 (70257898)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 感染 / 増殖 / カブモザイクウイルス / VPg / 翻訳開始因子 / アメリカヤマゴボウ / PAP / RIP / IRES / シロイヌナズナ / eIF(iso)4E / 翻訳 / 洋種山牛蒡 |
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| Research Abstract |
To elucidate the mechanism of RNA depurination, and to understand how PAP recognizes and targets various RNAs, the interactions between PAP and Turnip mosaic virus genome linked protein (VPg) were investigated. VPg can function as a cap analog in cap-independent translation, and potentially target PAP to uncapped IRES-containing RNA. In this work, fluorescence spectroscopy and HPLC techniques were used to quantitatively describe PAP depurination activity and PAP-VPg interactions. PAP binds to VPg with high affinity (29.5 nM); the reaction is enthalpically driven and entropically favored. Further, VPg is a potent inhibitor of PAP depurination of RNA in wheat germ lysate, and competes with structured RNA derived from tobacco etch virus (TEV) for PAP binding. VPg may confer an evolutionary advantage by suppressing one of the plant defense mechanisms, and also suggests the possible use of this protein against the cytotoxic activity of RIPs.
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