Development of a reverse-genetics system for Tomato spotted wilt virus
| Project/Area Number |
23658043
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
ISHIKAWA Masayuki 独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, ユニット長 (70192482)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 感染・増殖 / 植物 / マイナス鎖RNAウイルス / 複製 / 転写 / 宿主因子 |
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| Research Abstract |
Toward establishing an experimental system in which infection with Tomato spotted wilt virus(TSWV) can be initiated from its cDNA, we expressed TSWV nucleocapsid protein and RNA polymerase(the L protein) in Escherichia coli and Saccharomyces cerevisiae, respectively. We purified these proteins and mixed with in vitro-synthesized TSWV RNA in various conditions, however, we could not detect RNA synthesis. We also constructed plasmids to express TSWV-coded proteins and RNA in vivo, and introduced these plasmids into Nicotiana benthamiana leaves by the Agro-infiltration method, infection with TSWV did not occur. In the course of these trials, we obtained results that suggest that the translation elongation factor eEF1A plays important roles in mRNA transcription of TSWV.
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Report
(2 results)
Research Products
(2 results)
