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Development of a reverse-genetics system for Tomato spotted wilt virus

Research Project

Project/Area Number 23658043
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant pathology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

ISHIKAWA Masayuki  独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, ユニット長 (70192482)

Project Period (FY) 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords感染・増殖 / 植物 / マイナス鎖RNAウイルス / 複製 / 転写 / 宿主因子
Research Abstract

Toward establishing an experimental system in which infection with Tomato spotted wilt virus(TSWV) can be initiated from its cDNA, we expressed TSWV nucleocapsid protein and RNA polymerase(the L protein) in Escherichia coli and Saccharomyces cerevisiae, respectively. We purified these proteins and mixed with in vitro-synthesized TSWV RNA in various conditions, however, we could not detect RNA synthesis. We also constructed plasmids to express TSWV-coded proteins and RNA in vivo, and introduced these plasmids into Nicotiana benthamiana leaves by the Agro-infiltration method, infection with TSWV did not occur. In the course of these trials, we obtained results that suggest that the translation elongation factor eEF1A plays important roles in mRNA transcription of TSWV.

Report

(2 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (2 results)

All 2012

All Presentation (2 results)

  • [Presentation] トマト黄化えそウイルスのmRNA合成を活性化する宿主因子の探索2012

    • Author(s)
      薦田圭介, 石橋和大, 河村和恵, 不川雅之, 飯哲夫
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] トマト黄化えそウイルスのmRNA合成を活性化する宿主因子の探索2012

    • Author(s)
      薦田圭介、石橋和大、河村和恵、石川雅之、飯哲夫
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡
    • Related Report
      2011 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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