Search for the novel nematode pathogens

• Project/Area Number: 23658051
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied entomology
• Research Institution: Saga University
• Principal Investigator: YOSHIGA Toyoshi 佐賀大学, 農学部, 准教授 (00312231)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 線虫 / ウイルス / 病理 / 病原体 / 微生物 / 感染

Research Abstract

Survey of novel pathogens and parasites against nematodes were conducted using different approaches. Using C. elegans strains as a bait, nematode pathogens were screened. Some nematodes with morphological abnormal at the posterior part or vulva were seen but they seems not to be infected pathogens. On the other hands, different species and isolates of nematodes were screened using long chain double-stranded RNA (dsRNA) as a marker, and long chain dsRNA were detected from 3 nematode isolates, which suggests the possibility of discovery of novel types of nematode viruses.