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Toward a new drug resitance mechanism by excretion in insects

Research Project

Project/Area Number 23658054
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

NODA Hiroaki  独立行政法人農業生物資源研究所, 昆虫微生物機能研究ユニット, 研究専門員 (40343991)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Yukiko  農業生物資源研究所, 昆虫科学研究領域, 主任研究員 (80414944)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsABC トランスポーター / トビイロウンカ / 殺虫剤抵抗性 / 薬剤耐性 / ABCトランスポーター
Research Abstract

Multidrug resistance proteins (ABC transporters) are well known in mammals and bacteria. In insects, multidrug resistance proteins are not well characterized, although these proteins may be related to insecticide resistance in insect pests. ABC transporters that are involved in drug excretion were explored based on sequence similarity, and two candidate genes were cloned. An ABC transporter gene, whose expression was up-regulated upon the insecticide treatment, was also found by microarray analyses. These three ABC transporter genes were introduced into insect cell line (Sf9) and cytotoxicity assay is performing to know whether these genes work for drug excretion.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (31 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 1 results) Presentation (24 results) (of which Invited: 11 results) Remarks (3 results)

  • [Journal Article] KONAGAbase : a genomic and transcriptomic database for the diamondback moth, Plutella xylostella2013

    • Author(s)
      Jouraku A, Yamamoto K, Kuwazaki S, Urio M, Suetsugu Y, Narukawa J, Miyamoto K, Kurita K, Kanamori H, Katayose Y, Matsumoto T, Noda H
    • Journal Title

      BMC Genomics

      Volume: 14 Pages: 464-464

    • Related Report
      2013 Final Research Report
  • [Journal Article] High mortality caused by high dose of dsRNA in the green rice leafhopper Nephotettix cincticeps (Hemiptera : Cicadellidae)2013

    • Author(s)
      Tomizawa M, Noda H
    • Journal Title

      Appl. Entomol. Zool

      Volume: 48 Pages: 553-559

    • NAID

      40019883918

    • Related Report
      2013 Final Research Report
  • [Journal Article] High mortality caused by high dose of dsRNA in the green rice leafhopper Nephotettix cincticeps (Hemiptera: Cicadellidae)2013

    • Author(s)
      Tomizawa M and Noda H
    • Journal Title

      Applied Entomology and Zoology

      Volume: 48 Issue: 4 Pages: 553-559

    • DOI

      10.1007/s13355-013-0211-5

    • NAID

      40019883918

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A single amino acid mutation in an ABC transporter gene causes resistance to Bt toxin Cry1Ab in the silkworm2012

    • Author(s)
      Atsumi S, Miyamoto K, Yamamoto K, Narukawa J, Kawai S, Sezutsu H, Kobayashi I, Uchino K, Tamura T, Mita K, Kadono-Okuda K, Wada S, Kanda K, Goldsmith MR, Noda H
    • Journal Title

      Bombyx mori. PNAS

    • Related Report
      2013 Final Research Report
  • [Presentation] 各種殺虫剤処理間でのトビイロウンカ発現遺伝子変化の比較2014

    • Author(s)
      野田博明・中村有希・末次克行・山本公子・中平国光
    • Organizer
      第58回日本応用動物昆虫学会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] トビイロウンカのニコチン性アセチルコリン受容体(nAChR)遺伝子の識別と発現解析2013

    • Author(s)
      河合佐和子・小泉蓉子・野田博明
    • Organizer
      第57回日本応用動物昆虫学会
    • Place of Presentation
      日本大学藤沢キャンパス
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 殺虫剤抵抗性の機構と抵抗性マネージメント2013

    • Author(s)
      野田博明
    • Organizer
      第57回日本応用動物昆虫学会
    • Place of Presentation
      日本大学藤沢キャンパス
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Invited
  • [Presentation] 虫の薬剤防除における課題 : ゲノム科学からのアプローチ2012

    • Author(s)
      野田博明
    • Organizer
      日本学術会議公開シンポジウム「植物保護におけるゲノム科学の利用」
    • Place of Presentation
      日本学術会議講堂
    • Year and Date
      2012-11-13
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Molecular and genomics approach against planthopper problems2012

    • Author(s)
      Noda H
    • Organizer
      Seminar in Zhejiang University
    • Place of Presentation
      中国浙江大学昆虫科学院
    • Year and Date
      2012-03-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] トビイロウンカのイミダクロプリド抵抗性機構の現状 : P450 酵素遺伝子の高発現2012

    • Author(s)
      野田博明・中村有希・河合佐和子・末次克行・古崎利紀・篠田徹郎・山本公子・真田幸代・松村正哉・中平国光・尾添嘉久
    • Organizer
      日本農薬学会第37回大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 殺虫剤処理により発現変動するトビイロウンカ遺伝子のマイクロアレイ解析2012

    • Author(s)
      中村有希・末次克行・古崎利紀・篠田徹郎・真田幸代・松村正哉・山本公子・野田博明
    • Organizer
      第56回日本応用動物昆虫学会
    • Place of Presentation
      近畿大学
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] RNAi によるトビイロウンカの新規殺虫剤標的遺伝子の探索2012

    • Author(s)
      篠田徹郎・粥川琢巳・岡田千恵子・田中良明・大門高明・末次克行・山本公子・野田博明
    • Organizer
      第56回日本応用動物昆虫学会
    • Place of Presentation
      近畿大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Progress in genome sequencing of the brown planthopper, Nilaparvata lugens2012

    • Author(s)
      Suetsugu Y, Daimon T, Jouraku A, Kobayashi T, Nakamura Y, Kuwazaki S, Tanaka Y, Tanaka H, Shiotsuki T, Shinoda T, Yamamoto K, Noda H
    • Organizer
      Arthropod Genomics 2012
    • Place of Presentation
      Kansas City, アメリカ合衆国
    • Related Report
      2013 Final Research Report
  • [Presentation] Imidacloprid resistance in the brown planthopper : Genes for nAChR subunits and P450 enzymes2012

    • Author(s)
      Noda H, Nakamura Y, Kawai S, Koizumi Y, Suetsugu Y, Kozaki T, Daimon T, Shinoda T, Yamamoto K, Sanada S, Matsumura M, Nakahira K, Ozoe Y
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Daegu, 韓国
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Planthopper genomics for alleviating its outbreak and critical damage to rice2012

    • Author(s)
      Noda H
    • Organizer
      International Symposium on Rice Functional Genomics
    • Place of Presentation
      Chiang Mai, タイ
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Genomic studies of rice planthoppers for insecticide resistance management2012

    • Author(s)
      Noda H
    • Organizer
      NARO International Symposium 2012 "New insight into insecticide resistance of rice planthoppers and the insect- borne viruses"
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Progress in genome sequencing of the brown planthopper, Nilaparvata lugens.2012

    • Author(s)
      Suetsugu Y, Daimon T, Jouraku A, Kobayashi T, Nakamura Y, Kuwazaki S, Tanaka Y, Tanaka H, Shiotsuki T, Shinoda T, Yamamoto K, Noda H
    • Organizer
      Arthropod Genomics 2012
    • Place of Presentation
      Kansas City, アメリカ合衆国
    • Related Report
      2012 Research-status Report
  • [Presentation] Imidacloprid resistance in the brown planthopper: Genes for nAChR subunits and P450 enzymes.2012

    • Author(s)
      Noda H, Nakamura Y, Kawai S, Koizumi Y, Suetsugu Y, Kozaki T, Daimon T, Shinoda T, Yamamoto K, Sanada S, Matsumura M, Nakahira K, Ozoe Y
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Daegu, 韓国
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Planthopper genomics for alleviating its outbreak and critical damage to rice.2012

    • Author(s)
      Noda H
    • Organizer
      International Symposium on Rice Functional Genomics
    • Place of Presentation
      Chiang Mai, タイ
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Genomic studies of rice planthoppers for insecticide resistance management.2012

    • Author(s)
      Noda H
    • Organizer
      NARO International Symposium 2012 "New insight into insecticide resistance of rice planthoppers and the insect-borne viruses"
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] トビイロウンカのイミダクロプリド抵抗性機構の現状:P450酵素遺伝子の高発現2012

    • Author(s)
      野田博明・中村有希・河合佐和子・末次克行・古崎利紀・篠田徹郎・山本公子・真田幸代・松村正哉・中平国光・尾添嘉久
    • Organizer
      日本農薬学会第37回大会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular and genomics approach against planthopper problems2012

    • Author(s)
      Noda Hiroaki
    • Organizer
      Seminar in Zhejiang University(招待講演)
    • Place of Presentation
      中国浙江大学昆虫科学院
    • Related Report
      2011 Research-status Report
  • [Presentation] RNAiによるトビイロウンカの新規殺虫剤標的遺伝子の探索2012

    • Author(s)
      篠田徹郎・粥川琢巳・岡田千恵子・田中良明・大門高明・末次克行・山本公子・野田博明
    • Organizer
      第56回日本応用動物昆虫学会
    • Place of Presentation
      近畿大学
    • Related Report
      2011 Research-status Report
  • [Presentation] イネの最大の害虫ウンカ : 研究の歴史と現状, そして今後2011

    • Author(s)
      野田博明
    • Organizer
      NIASシンポジウム「ウンカ防除の現状と展望」
    • Place of Presentation
      秋葉原コンベンションセンター
    • Year and Date
      2011-09-09
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ビイロウンカのnAChR 遺伝子の単離と機能的発現2011

    • Author(s)
      野田博明・旭美穂・小泉蓉子・真田幸代・松村正哉・松田一彦・中平国光
    • Organizer
      農林害虫防除研究会
    • Place of Presentation
      山口市
    • Related Report
      2013 Final Research Report
  • [Presentation] トビイロウンカのnAChR遺伝子の単離と機能的発現2011

    • Author(s)
      野田博明・旭美穂・小泉蓉子・真田幸代・松村正哉・松田一彦・中平国光
    • Organizer
      農林害虫防除研究会
    • Place of Presentation
      山口市
    • Related Report
      2011 Research-status Report
  • [Presentation] イネの最大の害虫ウンカ:研究の歴史と現状、そして今後2011

    • Author(s)
      野田博明
    • Organizer
      NIASシンポジウム「ウンカ防除の現状と展望」(招待講演)
    • Place of Presentation
      秋葉原コンベンションセンター
    • Related Report
      2011 Research-status Report
  • [Presentation] 虫の薬剤防除における課題:ゲノム科学からのアプローチ

    • Author(s)
      野田博明
    • Organizer
      日本学術会議 公開シンポジウム「植物保護におけるゲノム科学の利用」
    • Place of Presentation
      日本学術会議講堂
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.k.u-tokyo.ac.jp/pros/person/hiroaki_noda/hiroaki_noda.htm

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.ib.k.u-tokyo.ac.jp/NIAS/insect/i_index.html

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.nias.affrc.go.jp/renkei_daigakuin2/i_index.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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