Global systems of symbiotic nitrogen-fixing bradyrhizobia by comparative genomics of phylogenetic super-neighbors
| Project/Area Number |
23658057
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 共生 / ゲノム比較 / 根粒菌 / Bradyrhizobium / 窒素固定 / 土壌低栄養細菌 / タンパク質分泌系 / エンドファイト / 比較ゲノム / 土壌微生物 |
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| Research Abstract |
Global systems of symbiotic nitrogen-fixing bradyrhizobia were conducted by comparative genomics of phylogenetic super-neighbors of the bacteria. Soil-dwelling Bradyrhizobium sp. strain S23321 lacked symbiosis island with high genomic co-linearity with B. japonicum USDA110, suggesting an ancestral-type genome of symbiotic bradyrhizobia. On the other hand, the genome analysis suggested soil-dwelling Agromonas oligotrophica (Bradyrhizobium oligotrophicum) is a nitrogen-fixing symbiont of Aechynomeny indica. Indeed, all texted strains of A. oligotrophica are able to nodulate A. indica with symbiotic nitrogen fixation. In addition, the rhcJ and ttsI mutants of B. japonicum for the type III protein secretion system (T3SS) gained the ability to nodulate Rj2 soybean, suggesting the effectors secreted via T3SS trigger incompatibility between these two partners.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] A Rice Gene for Microbial Symbiosis, Orysa sativa CCaMK, Reduces CH_4 Flux in a Paddy Field with Low Nitrogen Input2014
Author(s)
Zhihua Bao, Aya Watanabe, Kazuhiro Sasaki, Takashi Okubo, Takeshi Tokida, Dongyan Liu, Seishi Ikeda, Haruko Imaizumi-Anraku, Susumu Asakawa, Tadashi Sato, Hisayuki Mitsui, Kiwamu Minamisawa
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Journal Title
Applied and Environmental Microbiology
Volume: VOL.80
Issue: 6
Pages: 1995-2003
DOI
Related Report
Peer Reviewed
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[Journal Article] Low Nitrogen Fertilization Adapts Rice Root Microbiome to Low Nutrient Environment by Changing Biogeochemical Functions2014
Author(s)
Seishi Ikeda, Kazuhiro Sasaki, Takashi Okubo, Akifumu Yamashita, Kimihiro Terasawa, Zhihua Bao, Dongyan Liu, Takeshi Watanabe, Jun Murase, Susumu Asakawa, Shima Eda, Hisayuki Mitsui, Tadashi Sato, and Kiwamu Minamisawa
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Journal Title
Microbes and Environments
Volume: 29
Issue: 1
Pages: 50-59
DOI
NAID
ISSN
1342-6311, 1347-4405
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The type III Secretion System of Bradyrhizobium japonicum USDA122 mediates symbiotic incompatibility with Rj2 soybean plants2013
Author(s)
Tsukui T, Eda S, Kaneko T, Sato S,Okazaki S, Kakizaki-Chiba K, Itakura M,Mitsui H, Yamashita A, Terasawa K,Minamisawa K
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Journal Title
Appl Environ Microbiol
Volume: 79
Issue: 3
Pages: 1048-1051
DOI
Related Report
Peer Reviewed
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[Journal Article] Mitigation of nitrous oxide emissions from soils by Bradyrhizobium japonicum inoculation.2013
Author(s)
Itakura M, Uchida Y, Akiyama H, Takada-Hoshino Y, Shimomura Y, Morimoto S, Tago K, Wang Y, Hayakawa C, Uetake Y, Sanchez C, EdaS, Hayatsu M, Minamisawa K.
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Journal Title
Nature Climate Change
Volume: 3
Issue: 3
Pages: 208-212
DOI
Related Report
Peer Reviewed
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[Presentation] Genome analysis suggests that the soil oligotrophic bacterium Agromonas oligotrophica (Bradyrhizobium oligotrophicum) is a nitrogen-fixing symbiont of Aeschynomene indica2013
Author(s)
Takashi Okubo, Shohei Fukushima, Manabu Itakura, Kenshiro Oshima, Aphakorn Longtonglang, Neung Teaumroong, Hisayuki Mitsui, Masahira Hattori, Reiko Hattori, Tsutomu Hattori, Kiwamu Minamisawa
Organizer
18th International Congress on Nitrogen Fixation
Place of Presentation
Miyazaki, Japan
Related Report
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[Presentation] Soil oligotrophic bacterium, Agromonas oligotrophica (Bradyrhizobium oligotrophicum) is nitrogen-fixing symbiont of Aeschynomene indica as suggested by genome analysis2012
Author(s)
Takashi Okubo, Shohei Fukusima, Manabu Itakura, Kenshiro Ohshima, Aphakorn Longtonlang, Neung Teaumroong, Hisayuki Mitsui, Masahira Hattori, Reiko Hattori, Tsutomu Hattori, and Kiwamu Minamisawa
Organizer
The 2nd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
Place of Presentation
Phuket, Thailand
Related Report
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