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Methodological development of plant root and microorganism interaction though visualization of rhizosphere effect

Research Project

Project/Area Number 23658062
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

SHINANO Takuro  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・生産環境研 究領域, 上席研究員 (20235542)

Co-Investigator(Kenkyū-buntansha) FUJIMAKI Shu  独立行政法人 日本原子力研究開発機構, 量子ビーム応用研究部門, 研究副主幹 (20354962)
NAKAMURA Takuji  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・生産環境研 究領域, 主任研究員 (60399425)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords土壌環境 / 根圏 / 根圏土壌 / 根分泌物 / 光合成産物 / 土壌微生物 / メタボローム / メタゲノム
Research Abstract

Short half-life period radio nuclei:^<11>C was introduced to the shoot as carbon dioxide by photosynthesis, then the distribution of ^<11>C to the rhizosphere was analyzed. It was demonstrated that the distribution was most pronounced to the rhizosphere very close to the area of lateral root. The analytical development of collection and analysis of root secreting low molecular compounds and high molecular compounds (proteins) were developed. Phylogenetical and functional analysis of microorganism from rhizosphere soil very close to the root was performed by using pyrosequencing method on microorganism DNA and the obtained result shows that some functional gene fluctuation may explain the difference of plant growth.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (41 results)

All 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (23 results) (of which Invited: 3 results) Book (4 results) Remarks (1 results)

  • [Journal Article] 植物根圏微生物の機能解析手法開発2013

    • Author(s)
      信濃卓郎
    • Journal Title

      日草誌

      Volume: 59(1) Pages: 70-73

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metagenomic Analysis of the Rhizosphere Soil Microbiome with Respect to Phytic Acid Utilization2013

    • Author(s)
      Yusuke Unno
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 1 Pages: 120-127

    • DOI

      10.1264/jsme2.ME12181

    • NAID

      10031138552

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice plant under phosphorus deficiency2013

    • Author(s)
      Tawaraya, K., Horie, R., Saito, A., Shinano, T., Wagatsuma, T., Saito, K. and Oikawa, A
    • Journal Title

      Journal of Plant Nutrition

      Volume: 36(7) Pages: 1138-1159

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice plant under phosphorus deficiency2013

    • Author(s)
      Tawaraya, K., Horie, R., Saito, A., Shinano, T., Wagatsuma, T., Saito, K., Oikawa, A
    • Journal Title

      J Plant Nutr

      Volume: 36 Issue: 7 Pages: 1138-1159

    • DOI

      10.1080/01904167.2013.780613

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 微生物資材の可能性と課題2013

    • Author(s)
      信濃卓郎
    • Journal Title

      ニューカントリー

      Volume: 70 Pages: 126-127

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Effect of exogenous phosphatase and phytase activities on organic phosphate mobilization in soils with different phosphate adsorption capacities2012

    • Author(s)
      Maruyama, H.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 58 Pages: 41-51

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comprehensive analysis of mitochondria in roots and hypocotyls of soybean under flooding stress using proteomics and metabolomics techniques.2012

    • Author(s)
      Komatsu, S.
    • Journal Title

      Journal of Proteome Research

      Volume: 10 Pages: 3933-4004

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean.2012

    • Author(s)
      Lee, J.W.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 113 Pages: 262-268

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress.2012

    • Author(s)
      Ahsan, N.
    • Journal Title

      Amino Acids

      Volume: 42 Pages: 317-327

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of secreted proteins from aseptically grown rice2011

    • Author(s)
      Shinano, T., Komatsu, S., Yoshimura, T., Tokutake, S., Kong, F-J., Watanabe, T., Wasaki, J. and Osaki, M
    • Journal Title

      Phytochemistry

      Volume: 72(4-5) Pages: 312-320

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of secreted proteins from aseptically grown rice.2011

    • Author(s)
      Shinano, T.
    • Journal Title

      Phytochemistry

      Volume: 73 Pages: 312-320

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ionomic response of Lotus japonicas to different root-zone temperature.2011

    • Author(s)
      Quadir, Q.F.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 57 Pages: 221-232

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 植物の有害元素2011

    • Author(s)
      信濃卓郎
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 82 Pages: 504-510

    • Related Report
      2011 Research-status Report
  • [Presentation] 植物と土壌の接点?根圏?をめぐる研究2012

    • Author(s)
      信濃卓郎
    • Organizer
      第30回農薬環境科学研究会
    • Place of Presentation
      前橋
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of microbial community structure and function in the field soil by using a comprehensive analysis methods of soilDNA and RNA2012

    • Author(s)
      Unno, Y. and Shinano, T
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋
    • Related Report
      2012 Final Research Report
  • [Presentation] Behavior of Rhizosphere2012

    • Author(s)
      Shinano, T
    • Organizer
      北海道国際農学会議 2012
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物根圏微生物の機能解析手法開発2012

    • Author(s)
      信濃卓郎
    • Organizer
      日本草地学会
    • Place of Presentation
      江別
    • Related Report
      2012 Final Research Report
  • [Presentation] Nano LC-MS analysis onroot secreting proteins2012

    • Author(s)
      Shinano, T, 他
    • Organizer
      Roots to theFuture conference, ISRR2012
    • Place of Presentation
      Dundee, England
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of microbial community structure and function in the field soil by using a comprehensive analysis methods of soil DNA and RNA2012

    • Author(s)
      Unno, Y.
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      豊橋技術大学(豊橋市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 植物根圏微生物の機能解析手法開発2012

    • Author(s)
      信濃卓郎
    • Organizer
      日本草地学会
    • Place of Presentation
      酪農学園大学(江別市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Behavior of Rhizosphere2012

    • Author(s)
      Shinano, T.
    • Organizer
      北海道国際農学会議2012
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 植物と土壌の接点ー根圏ーをめぐる研究2012

    • Author(s)
      信濃卓郎
    • Organizer
      農業環境科学研究会
    • Place of Presentation
      マロウドイン熊谷(熊谷市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Metatranscriptomic approach to analyze freeze-thaw stress on the structure and function of soil microbiota with respect to denitrification process2012

    • Author(s)
      Unno, Y.
    • Organizer
      Plant and Microbe Adaptation to Cold 2012
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミヤコグサAtMOT1相同遺伝子の機能解析2012

    • Author(s)
      箱山雅生
    • Organizer
      第53回日本植物整理学会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Presentation] リン欠乏及びアルミニウム障害時におけるシロイヌナズナ野生株およびリンゴ酸分泌変異株の根浸出物の解析2012

    • Author(s)
      丸山隼人
    • Organizer
      第53回日本植物整理学会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Ionomic study of soybean seed affected by previous cropping with mycorrhizal plant, manure and P applications2012

    • Author(s)
      Shimin Shsa
    • Organizer
      第53回日本植物整理学会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 微生物全体から見たわずかな違いがもたらす植物生産性への意義2011

    • Author(s)
      信濃卓郎
    • Organizer
      日本土壌微生物生態学会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Metagenomic approach reveals the rhizosphere function on phytate utilization.2011

    • Author(s)
      Shinano, T.
    • Organizer
      Rhizosphere 3
    • Place of Presentation
      Perth, Australia
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of Phosphate Utilization in Arabidopsis thaliana Natural Accessions Grown on Soil2011

    • Author(s)
      Furutani, A.
    • Organizer
      Rhizosphere 3
    • Place of Presentation
      Perth, Australia
    • Related Report
      2011 Research-status Report
  • [Presentation] Metagenomic analysis on the rhizosphere soil microbiome.2011

    • Author(s)
      Shinano, T.
    • Organizer
      3rd International Workshop "Advances in Science and Technology of Natural Resources"(招待講演)
    • Place of Presentation
      Pucon, CHile
    • Related Report
      2011 Research-status Report
  • [Presentation] Phosphorus starvation changes the metabolic flow in rice.2011

    • Author(s)
      Shinano, T.
    • Organizer
      3rd International Symposium on Frontiers in Agriculture Proteome Research(招待講演)
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 土壌に根ざした作物栄養学の貢献―農業再考―2011

    • Author(s)
      信濃卓郎
    • Organizer
      日本学術振興会・日本土壌肥料学会共催シンポジウム(招待講演)
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 根の世界2011

    • Author(s)
      信濃卓郎
    • Organizer
      サッポロ農学校2011(招待講演)
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 微生物全体から見たわずかな違いがもたらす植物生産性への意義2011

    • Author(s)
      信濃卓郎
    • Organizer
      日本土壌微生物生態学会シンポジウム(招待講演)
    • Place of Presentation
      京都大学
    • Related Report
      2011 Research-status Report
  • [Presentation] トウモロコシの有機質肥料効率利用メカニズムの解明2011

    • Author(s)
      太平佳菜
    • Organizer
      日本土壌肥料学会北海道支部会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 飽和土壌中の一酸化二窒素濃度の低温領域での温度依存性と凍結・融解の効果2011

    • Author(s)
      岩田幸良
    • Organizer
      土壌物理学会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Research-status Report
  • [Book] In: Frans J. de Brjin (ed.) MolecularMicrobial Ecology of the Rhizosphere Wiley-Blackwell Publishers2013

    • Author(s)
      Unno, Y. and Shinano, T
    • Publisher
      Metagenomic analysis on the rhizosphere soil microbial community
    • Related Report
      2012 Final Research Report
  • [Book] Metagenomic analysis on the rhizosphere soil microbial community. In: Molecular Microbial Ecology of the Rhizosphere2013

    • Author(s)
      Unno, Y.
    • Total Pages
      1328
    • Publisher
      Wiley-Blackwell Publishers
    • Related Report
      2012 Annual Research Report
  • [Book] 土壌科学を基盤とする学術の動向と展開:Part1作物の生産基盤としての土壌科学2012

    • Author(s)
      三枝雅彦・太田健・信濃卓郎・南澤究・稲村達也
    • Publisher
      土肥誌
    • Related Report
      2012 Final Research Report
  • [Book] Frontiers in Agriculture Proteome Research2012

    • Author(s)
      Shinano, T.
    • Publisher
      NARO Institute of Crop Science
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://cse.naro.affrc.go.jp/shinano/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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