| Project/Area Number |
23658066
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University (2012)
Yamagata University (2011) |
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Principal Investigator |
KAMIO Yoshiyuki 東北大学, 大学院・生命科学研究科, 客員研究員 (00109175)
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| Co-Investigator(Kenkyū-buntansha) |
金子 淳 東北大学, 農学研究科, 准教授 (30221188)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | プラズマローゲン / 認知症予防 / 口腔内細菌 / γセクレターゼ / βアミロイド / プラズマローゲンリン脂質 / ヒト認知症予防 / Selenomonas 属細菌 / 酵母ミクロソ－ム画分 / ベータアミロイド / Selenomonas sputigena / ヒト口腔内細菌 / γ-セクレターゼ / 酵母ミクロソーム画分 |
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| Research Abstract |
We found plasmalogen phospholipids (Pls), which are known to preventeAlzheimer disease (AD), in a human oral bacteria, Selenomonas sputigena. It has been known that Pls decrease in brain of AD patient. γ-Secretase(γS) is known to cleave amyloid precursor protein toproduce amyloid β peptides (Aβ) that is deposited in the brain of Alzheimer disease. Here we examined a relationship between phospholipid composition and production of γ-secretase in human brain, by in vitro assay system using Saccharomyces cerevisiae, which was established by Futai et al. The followings are evident, (1) Aβ-production is enhanced by the addition of phosphatidyl choline (PC), and (2) addition of phosphatidyl ethanolamine Pls (EPPls) to in vitro assay system prevented γS activity. Thus we concluded that the reduction of EPPls in the phospholipid fraction in human brain enhances the γS activity and causes Aβ-production and/or accumulation in AD patient. This is a first report in theworld.
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