Functional analysis of the autophagy specific to filamentous fungi

• Project/Area Number: 23658069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: KITAMOTO Katsuhiko 東京大学, 大学院・農学生命科学研究科, 教授 (20272437)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 麹菌 / オートファジー / 糸状菌

Research Abstract

Molecular biology of autophagy has started using yeast S. cerevisiae, and its important roles in eukaryotes have been revealed in not only yeast but also mammalian cells. However, little is known about filamentous fungi. Filamentous fungi such a Koji mold, Aspsergillus oryzae differentiates into conidia formation upon environmental change. Many autophagy-related genes from A. oryzae were isolated and characterized. Function and roles of autophagy specific to filamentous fungi have been analyzed.

Research Products

• [Journal Article] Enhanced production of bovine chymosin by autophagy deficiency in the filamentous fungus Aspergillus oryzae J (2013)
  • Author(s): Yoon,T.Kikuma, J. Maruyama, K. Kitamoto
  • Journal Title: PLoS ONE Volume: 8(4)
  • Peer Reviewed
• [Journal Article] Functional analysis of Aoatg1 and detection of the Cvt pathway in Aspergillus oryzae S. (2013)
  • Author(s): Yanagisawa, T. Kikuma, K. Kitamoto
  • Journal Title: FEMS Microbiol. Lett Volume: 338 Pages: 168-176
  • Peer Reviewed
• [Journal Article] Functional analysis of Aoatg1 and detection of the Cvt pathway in Aspergillus oryzae (2013)
  • Author(s): S. Yanagisawa, T. Kikuma, K. Kitamoto
  • Journal Title: FEMS Microbiol. Lett. Volume: 338 Pages: 168-176
  • Peer Reviewed
• [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy (2012)
  • Author(s): D. J. Klionsky,,,,K. Kitamoto,,,, B. Zuckerbraun
  • Journal Title: Autophagy Volume: 8 Pages: 445-544
  • Peer Reviewed
• [Journal Article] Autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus Aspergillus oryzae S. (2011)
  • Author(s): Kimura, J. Maruyama, T. Kikuma, M. Arioka, K.Kitamoto Biochem.
  • Journal Title: Biophys. Res. Commun Volume: 406 Pages: 464-470
  • Peer Reviewed
• [Presentation] 麹菌A.oryzaeにおけるオートファジー欠損株を用いた異種タンパク質の生産性向上 尹載宇 (2012)
  • Author(s): 菊間隆志、柳沢晋、丸山潤一、北本勝ひこ
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
• [Presentation] 誘導プロモーターを用いたオートファジー制御による麹菌からの実用的異種タンパク質高生産宿主の造成 (2012)
  • Author(s): 菊間隆志、尹載宇、丸山潤一、北本勝ひこ
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
• [Presentation] 麹菌A.oryzaeにおける選択的オートファジー関連遺伝子Aoatg11の機能解析 (2012)
  • Author(s): 田所隆之、菊間隆志、北本勝ひこ
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
• [Presentation] 麹菌 A. oryzae における選択的オートファジー関連遺伝子 Aoatg11 の機能解析 (2012)
  • Author(s): 田所 隆之、菊間 隆志、北本 勝ひこ
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
• [Presentation] 麹菌 A. oryzae におけるオートファジー欠損株を用いた異種タンパク質の生産性向上 (2011)
  • Author(s): 尹 載宇、菊間 隆志、柳沢 晋、丸山 潤一、北本 勝ひこ
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
• [Book] GFPを用いた麹菌のオルガネラの可視化とオートファジー (2012)
  • Author(s): 菊間隆志、丸山潤一、北本勝ひこ
  • Publisher: 改訂版分子麹菌学、日本醸造協会
• [Book] 改訂版 分子麹菌学 (GFPを用いた麹菌のオルガネラの可視化とオートファジー) (2012)
  • Author(s): 菊間隆志、丸山潤一、北本勝ひこ
  • Publisher: 日本醸造協会
• [Book] 改訂版 分子麹菌学(GFPを用いた麹菌のオルガネラの可視化とオートファジー) (2012)
  • Author(s): 菊間隆志、丸山潤一、北本勝ひこ
  • Publisher: 日本醸造協会
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/Lab_Microbiology/hyousi.html
• [Remarks] http://park.itc.u-tokyo.ac.jp/Lab_Microbiology/
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/Lab_Microbiology/