Synthesis of functional metal nano particles based on bacterial metal metabolism

• Project/Area Number: 23658078
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: KAWAMOTO Jun 京都大学, 化学研究所, 助教 (90511238)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 応用微生物学 / 微生物機能 / 環境農学 / 金属ナノ粒子 / 極限環境微生物 / 金属呼吸 / 特殊環境微生物

Research Abstract

Metallic nanoparticles have huge potentials in nanotechnology. In this study, we attempted to develop the novel methods for metallic nanoparticles by using microorganisms. We have isolated a novel strain of Pseudomonas species, which produces nano-sized silver particles in the presence of silver nitrate at moderate temperatures, suggesting that this strain has an unique mechanism for metallic nanoparticle synthesis. In order to develop the efficient nano particle synthesis, we also analyzed the iron-respiratory mechanism of a psychrotrophic bacterium, Shewanella livingstonensis Ac10, which has flexible metal respiratory system at anaerobic conditions. Only when grown with divalent iron (ferric citrate), this strain produces a homolog of a phosphate selective porn, PhoE. In the presence of ferric citrate, a gene-disrupted mutant of phoE showed growth retardation, indicating that PhoE has an important role in iron-respiration mechanism of this strain.

Research Products

• [Journal Article] Occurrence of bacterial membrane microdomain at the cell division site enriched in phospholipids with polyunsaturated hydrocarbon chain. (2012)
  • Author(s): Sato, S., Kawamoto, J., Sato, S.B., Watanabe, B., Hiratake, J., Esaki, N., and Kurihara, T.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 29 Pages: 24113-21
  • DOI: 10.1074/jbc.m111.318311
  • NAID: 120005302081
  • Peer Reviewed
• [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures (2011)
  • Author(s): Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N., and Kurihara, T
  • Journal Title: Environmental Microbiology Volume: 13 Issue: 8 Pages: 2293-2298
  • DOI: 10.1111/j.1462-2920.2011.02487.x
  • Peer Reviewed
• [Presentation] Shewanella livingstonensis Ac10の金属呼吸における外膜タンパク質PhoEの機能 (2014)
  • Author(s): 丸山 沙織、川本 純、樽井 淳、王 玉、江崎 信芳、栗原 達夫
  • Organizer: 日本農芸化学学会 2014年度大会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-29
• [Presentation] 低温菌 Shewanella livingstonensis Ac10による金属還元機構の解析 (2013)
  • Author(s): 丸山 沙織, 川本 純, 樽井 淳, 王 玉, 江崎 信芳, 栗原 達夫
  • Organizer: 第14回 極限環境生物学会
  • Place of Presentation: 東京
  • Year and Date: 2013-10-26
• [Presentation] Physiological roles of eicosapentaenoic acid in the cold-adaptation mechanism of an Antarctic bacterium, Shewanella livingstonensis Ac10 (2012)
  • Author(s): KAWAMOTO Jun, KURIHARA Tatsuo
  • Organizer: 9th international congress on extremophiles
  • Place of Presentation: Sevilla, Spain
• [Presentation] 低温菌 Shewanella livingstonensis Ac10 の低温環境適応機構におけるエイコサペンタエン酸の生理機能 (2011)
  • Author(s): 川本純、栗原達夫
  • Organizer: 第12回極限環境生物学会年会
  • Place of Presentation: 長崎大学