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Global isolation and classification of Azospirillum species by a new method

Research Project

Project/Area Number 23658080
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionOkayama University

Principal Investigator

TANI Akio  岡山大学, その他部局等, 助教 (00335621)

Co-Investigator(Renkei-kenkyūsha) NAKAGAWA Tomoyuki  岐阜大学, 応用生物科学部, 准教授 (70318179)
MITSUI Ryoji  岡山理科大学, 理学部, 准教授 (60319936)
Research Collaborator NURETTIN Sahin  ムグラ大学(トルコ共和国), 教育学部, 教授
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords国際情報交換 / Azospirllum属細菌 / 窒素固定 / 根圏 / トルコ / Azospirillum属細菌 / 国際情報交流
Research Abstract

Azospirillum species is azotrophic bacteria symbiotic to plant roots, and capable of promoting plant growth. Isolation of this species is difficult, and their classification and relationship between their host plants is not well elucidated. This study aims to isolate the species as many as possible, to classify them using mass-spectrometry dependent method, and to give a new insight into the classification of the species by finding new species. We found that nitrogen-free medium under micro-aerobic condition is important for their isolation, and we isolated 60 isolates. They were subjected to mass spectrometry for precise classification. We also found some species capable of promoting rice growth by 20%.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (8 results)

All 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 8 results)

  • [Journal Article] Extensive reduction in cell viability and enhanced matrix production in Pseudomonas aeruginosa PAO1 flow biofilms treated with D-amino acid mixture2013

    • Author(s)
      Sanchez Z, Tani A, Kimbara K
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 79 Issue: 4 Pages: 1396-1399

    • DOI

      10.1128/aem.02911-12

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylobacterium haplocladii sp. nov., and Methylobacterium brachythecii sp. nov. isolated from bryophytes2013

    • Author(s)
      Tani A, Sahin N
    • Journal Title

      Int J. Syst. Evol. Microbiol

      Volume: 63 Issue: Pt_9 Pages: 3287-3292

    • DOI

      10.1099/ijs.0.048215-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dominant Colonization and Inheritance of <i>Methylobacterium</i> sp. Strain OR01 on Perilla Plants2013

    • Author(s)
      Mizuno M, Yurimoto H, Iguchi H, Tani A, Sakai Y
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 7 Pages: 1533-1538

    • DOI

      10.1271/bbb.130207

    • NAID

      10031190465

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of rhizosphere, rhizoplane and phyllosphere bacteria and fungi isolated from rice in Kenya for plant growth promoters2013

    • Author(s)
      Mwajita RM, Murage H, Tani A, Kahangi ME
    • Journal Title

      SpringerPlus

      Volume: 2 Issue: 1 Pages: 606-606

    • DOI

      10.1186/2193-1801-2-606

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mangrove sediment, a new source of potential biosurfactant-producing bacteria.2012

    • Author(s)
      Saimmai, A., Tani, A., Sobhon, V., & Maneerat, S.
    • Journal Title

      Annals of Microbiology

      Volume: in press Issue: 4 Pages: 1669-1679

    • DOI

      10.1007/s13213-012-0424-9

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Culturable bacteria in hydroponic cultures of moss Racomitrium japonicum and their potential as biofertilizers for moss production2011

    • Author(s)
      Tani, A., Akita, M., Murase, H., & Kimbara, K.
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 112 Issue: 3 Pages: 32-39

    • DOI

      10.1371/journal.pone.0033800

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata.2011

    • Author(s)
      Tani, A., Sahin, N., & Kimbara, K.
    • Journal Title

      International Journal of Sytematic and Evolutionary Microbiology

      Volume: in press. Issue: Pt_7 Pages: 1647-1652

    • DOI

      10.1099/ijs.0.033019-0

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum2011

    • Author(s)
      Tani, A., Sahin, N., & Kimbara, K.
    • Journal Title

      International Journal of Sytematic and Evolutionary Microbiology

      Volume: in press Issue: Pt_11 Pages: 2602-2607

    • DOI

      10.1099/ijs.0.037713-0

    • Related Report
      2011 Research-status Report
    • Peer Reviewed

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Published: 2011-08-05   Modified: 2019-07-29  

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