Ethanol production from-laDctic acid bySaccharomyces cerevisiae

• Project/Area Number: 23658082
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Sojo University
• Principal Investigator: TAGUCHI Hisataka 崇城大学, 生物生命学部, 准教授 (90212018)
• Co-Investigator(Kenkyū-buntansha): AKAMATSU Takashi 崇城大学, 生物生命学部, 教授 (50133567)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: バイオエタノール / Saccharomyces cerevisiae / D-乳酸 / JEN1 / DLD1 / DLD2 / DLD3 / 都市型バイオマス

Research Abstract

We found that Saccharomyces cerevisiae NAM34-4C produced significant amounts of ethanol from D-lactate as compared with L-lactate at pH 3.0, but not at pH 5.5. We also report the improvement of ethanol production from D-lactate by constitutive expression of Jen1p or Dld1p in S. cerevisiae.

Research Products

• [Journal Article] Ethanol production from D-lactic acid by lactic acid-assimilating Saccharomyces cerevisiae NAM34-4C (2013)
  • Author(s): Makoto Wakamatsu
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 116 Issue: 1 Pages: 85-90
  • DOI: 10.1016/j.jbiosc.2013.01.017
  • NAID: 110009634750
  • Peer Reviewed
• [Journal Article] Improvement of Ethanol Production from D-Lactic Acid by Constitutive Expression of Lactate Transporter Jen1p in Saccharomyces cerevisiae (2013)
  • Author(s): 若松 誠、谷 龍典、田口久貴、木田建次、赤松隆
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Pages: 1114-1116
  • NAID: 10031177608
  • Peer Reviewed
• [Journal Article] Improvement of Ethanol Production from D-Lactic Acid by Constitutive Expression of Lactate Transporter Jen1p in Saccharomyces cerevisiae (2013)
  • Author(s): Makoto Wakamatsu
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 印刷中
  • NAID: 10031177608
  • Peer Reviewed
• [Presentation] Saccharomyces cerevisiaeの乳酸資化・発酵におけるJEN1とADY2の役割 (2012)
  • Author(s): 田口久貴
  • Organizer: 日本生物工学会九州支部
  • Place of Presentation: 別府大学
  • Year and Date: 2012-12-01
• [Presentation] 変異型JEN1を導入したSaccharomyces cerevisiaeによる乳酸からのエタノール生産 (2012)
  • Author(s): 田口久貴
  • Organizer: 日本生物工学会九州支部
  • Place of Presentation: 別府大学
  • Year and Date: 2012-12-01
• [Presentation] ユビキチン欠損Saccharomyces cerevisiae変異株によるD-乳酸からのエタノール生産 (2012)
  • Author(s): 田口久貴
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
• [Presentation] Saccharomyces cerevisiaeの乳酸資化・発酵におけるJEN1とADY2の役割 (2012)
  • Author(s): 谷龍典
  • Organizer: 日本生物工学会九州支部大会
  • Place of Presentation: 別府大学
• [Presentation] 変異型JEN1を導入したSaccharomyces cerevisiaeによる乳酸からのエタノール生産 (2012)
  • Author(s): 若松 誠
  • Organizer: 日本生物工学会九州支部大会
  • Place of Presentation: 別府大学
• [Presentation] ユビキチン欠損Saccharomyces cerevisiae変異株によるD-乳酸からのエタノール生産 (2012)
  • Author(s): 若松 誠，木田建次，田口久貴，赤松 隆
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学
• [Presentation] 乳酸を資化?発酵できる実用酵母の構築:JEN1欠損株の発酵試験 (2011)
  • Author(s): 田口久貴
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京農工大学
• [Presentation] 乳酸を資化・発酵できる実用酵母の構築：JEN1欠損株の発酵試験 (2011)
  • Author(s): 若松 誠，木田建次，田口久貴，赤松 隆
  • Organizer: 2011年度日本生物工学会大会
  • Place of Presentation: 東京農工大学