Mannosylerythritol lipids is associated with filamentous growth and propagation of phyllosphere yeast Pseudozyma antarctica on plant surfaces.

• Project/Area Number: 23658083
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: National Institute for Agro-Environmental Sciences
• Principal Investigator: KITAMOTO Hiroko 独立行政法人農業環境技術研究所, その他部局等, 研究員 (10370652)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Shigenobu 独立行政法人農業環境技術研究所, 生物生態機能研究領域, 主任研究員 (90354125)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 酵母 / 植物 / 接着 / 拡散 / 葉面常在酵母 / 付着

Research Abstract

In this study we found that MELs (mannosyl erythritol lipids) produced by yeast Pseudozyma antarctica on plant surfaces could be expected to play a significant role in fungal morphological development and propagation on plant surfaces.

Research Products

• [Journal Article] Mannosylerythritol lipids secreted by phyllosphere yeast Pseudozyma antarctica is associated with its filamentous growth and propagation on plant surfaces. (2014)
  • Author(s): Yoshida S, Morita T, Shinozaki Y, Watanabe T, Sameshima-Yamashita Y, Koitabashi M, Kitamoto D & Kitamoto H
  • Journal Title: Appl Microbiol Biotechnol Volume: 未定 Issue: 14 Pages: 6419-6429
  • DOI: 10.1007/s00253-014-5675-x
  • Peer Reviewed
• [Journal Article] Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study. (2013)
  • Author(s): Shinozaki Y, Kikkawa Y, Sato S, Fukuoka T, Watanabe T, Yoshida S, Nakajima-Kambe T & Kitamoto HK
  • Journal Title: Appl Microbiol Biotechnol Volume: 97 Issue: 19 Pages: 8591-8598
  • DOI: 10.1007/s00253-012-4673-0
  • Peer Reviewed
• [Journal Article] Affinity Purification and characterization of a biodegradable plastic-degrading enzyme from a yeast isolated from the larval midgut of a stag beetle,Aegus laevicollis (2013)
  • Author(s): Suzuki K,Sakamoto H,Shinozaki Y,Tabata J,Watanabe T,Mochizuki A,Koitabashi M,Fujii T,Tsushima S,Kitamoto HK
  • Journal Title: Appl.Microbiol.Biotechnol Volume: 97 Issue: 10 Pages: 7679-7688
  • DOI: 10.1007/s00253-013-5454-0
• [Journal Article] Affinity purification and characterization of a biodegradable plastic-degrading enzyme from a yeast isolated from the larval midgut of a stag beetle, Aegus laevicollis. (2013)
  • Author(s): Suzuki K, Sakamoto H, Shinozaki Y, Tabata J, Watanabe T, Mochizuki A, Koitabashi M, Fujii T, Tsushima S & Kitamoto HK
  • Journal Title: Appl Microbiol Biotechnol Volume: 97 Issue: 17 Pages: 7679-7688
  • DOI: 10.1007/s00253-012-4595-x
  • Peer Reviewed
• [Journal Article] Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films. (2013)
  • Author(s): Shinozaki Y, Watanabe T, Nakajima-Kambe T, Kitamoto HK.
  • Journal Title: J Biosci Bioeng. Volume: 115 Issue: 1 Pages: 111-114
  • DOI: 10.1016/j.jbiosc.2012.08.010
  • Peer Reviewed
• [Journal Article] Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization. (2013)
  • Author(s): Shinozaki Y, Morita T, Cao XH, Yoshida S, Koitabashi M, Watanabe T, Suzuki K, Sameshima-Yamashita Y, Nakajima-Kambe T, Fujii T, Kitamoto HK.
  • Journal Title: Appl Microbiol Biotechnol. Volume: 97 Issue: 7 Pages: 2951-2959
  • DOI: 10.1007/s00253-012-4188-8
  • Peer Reviewed
• [Journal Article] Phyllosphere yeasts rapidly break down biodegradable plastics (2011)
  • Author(s): Kitamoto, H.K
  • Journal Title: AMB Express Volume: 1 Issue: 1 Pages: 1-11
  • DOI: 10.1186/2191-0855-1-44
  • Peer Reviewed
• [Presentation] Peseudozyma 属酵母が植物体上で分泌する糖脂質マンノシルエリトールリピッドは、生息域の拡大に関与する (2014)
  • Author(s): 北本宏子,吉田重信,森田友岳
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 2A09p11 明治大学(東京都)
• [Presentation] 植物常在菌による生分解性プラスチックの分解促進 (2014)
  • Author(s): 北本宏子,小板橋基夫,渡部貴志,篠崎由紀子,山下結香,鈴木健
  • Organizer: 日本化学会
  • Place of Presentation: 3F5-33 名古屋大学(愛知県)
  • Invited
• [Presentation] Yeasts on plant surfaces break down biodegradable plastics (2014)
  • Author(s): Hiroko KITAMOTO, Motoo KOITABASHI, Yukiko SHINOZAKI, Takashi WATANABE, Yuka Sameshima-YAMASHITA, Shigenobu YOSHIDA
  • Organizer: 26th International Conference on Yeast genetics and Molecular Biology
  • Place of Presentation: ドイツ フランクフルト
  • Invited
• [Presentation] Yeast on plant surfaces break down bodegradable plastics (2013)
  • Author(s): Kitamoto H,Koitobashi M,Shinozaki Y,Watanabe T,Sameshima-Yamashita Y,Yoshida S
  • Organizer: YEAST(26th International Conference on Yeat Genetics and Molecular Biology Book of Abstract)
  • Place of Presentation: S231 フランクフルト大学(ドイツ)
• [Presentation] Peseudozyma 属酵母が植物体上で分泌する糖脂質マンノシルエリトールリピッドは、生息域の拡大に関与する
  • Author(s): 北本宏子, 吉田重信, 森田友岳
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学
• [Presentation] 植物常在菌による生分解性プラスチックの分解促進
  • Author(s): 北本宏子, 小板橋基夫, 渡部貴志, 篠崎由紀子, 山下結香, 鈴木健
  • Organizer: 日本化学会
  • Place of Presentation: 名古屋大学
  • Invited
• [Patent(Industrial Property Rights)] 植物の病害抵抗性増強用又は植物病害防除用組成物及びそれらの使用方法 (2017)
  • Inventor(s): 北本宏子, 他7名
  • Industrial Property Rights Holder: 農研機構
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] 植物の病害抵抗性増強用又は植物病害防除用組成物及びそれらの使用方法 (2017)
  • Inventor(s): 北本宏子, 他7名
  • Industrial Property Rights Holder: 農研機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-519603
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 微生物農薬製剤 (2011)
  • Inventor(s): 吉田重信・北本宏子
  • Industrial Property Rights Holder: 吉田重信・北本宏子
  • Industrial Property Number: 2011-119946
  • Filing Date: 2011