| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
To replace the trichodiene synthase gene (Tri5) in the trichothecene gene cluster of Fusarium graminearum with a codon-optimized synthetic amorpha-4,11-diene synthase gene (ADS), we established a genome engineering technique of the fungus using the positive and negative selection system with hph::tk, a fusion gene of the hygromycin phosphotransferase gene (hph) and thymidine kinase gene (tk). At the same time, we have identified compound 1 that markedly enhances sesquiterpene production by screening of compounds that elevate the level of trichothecene production. By employing the use of these tools, a transgenic F. graminearum strain carrying ADS was established, and the expression level of Tri4 and production level of the precursor amorphadiene are under investigation.
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