Regulation of intranuclear arrangement of genes and construction of cells which highly express recombinant proteins
| Project/Area Number |
23658091
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011-04-28 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 動物細胞 / 遺伝子発現 / DNAメチル化 / ヘテロクロマチン / DNA複製 / ゼブラフィッシュ / 核内配置 / エピジェネティクス / DNA損傷 / 核マトリックス |
|---|
| Outline of Final Research Achievements |
For the purpose of establishment of cells with efficient expression of recombinant proteins, intranuclear genome dynamics and the genome editing method were investigated. The following results were obtained: The intranuclear arrangement of genes during adipocyte differentiation was not changed even if they expressed. Overexpression of hemimethylated DNA binding protein UHRF1 induced the abnormal shape of the nucleus. Folate deficiency causes the DNA hypomethylation and induces the DNA damage. The specific large genomic region can be deleted by a CRISPR/Cas9 system. Various basic data on intranuclear DNA replication dynamics of Zebrafish cultured cells have been collected.
|
Report
(5 results)
Research Products
(12 results)
