Hydrogen production using nitrogenase driven by light energy
| Project/Area Number |
23658097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Nihon University Junior College |
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Principal Investigator |
SHIZAWA Yasuhiko 日本大学短期大学部, 生物資源学科, 講師 (30413131)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ニトロゲナーゼ / 水素ガス / 光エネルギー / 水素 / 放線菌 / 国際情報交換（ドイツ連邦共和国） / バイオリアクター |
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| Research Abstract |
Our research goal is the construction of hydrogen evolution system which is able to work by solar energy. We selected a bacterium whose name is Streptomyces thermoautotrophicus as an ideal candidate for this aim because of the features of its nitrogen reduction system. This bacterium normally grows with carbon monoxide as a sole carbon source at relatively high temperature, and also has a rather unique nitrogenase than generally known one. Since this nitrogenase functions in the severe environment; at relatively high temperature and under oxygen, it will be available for bioreactor. We decided to utilize this nitrogenase system as a recombinant protein synthesized in E.coli. The corresponding genes were chemically synthesized and cloned into the expression vector. This approach may allow us to build a foundation for the construction of solar energy dependent hydrogen evolution apparatus using this nitrogenase system.
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Report
(4 results)
Research Products
(4 results)
