| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
Utilizing the databases for known allergens (molecules causative of allergic diseases) and proteins in plant seeds, molecular structures were compared between the known allergens and the plant seed proteins. Then, the seed proteins identified to be structurally similar to any known allergens were subjected to the assay for binding with antibodies (proteins with allergen binding ability) from allergic patients. Many of the identified proteins showed the antibody binding, whereas some showed almost no binding. Thus, the strategy adopted in this study was shown to be effective to evaluate allergenic potential of novel proteins. It was also suggested that in the case of food allergens the susceptibility of proteins to denaturation or digestive degradation have to be considered for the evaluation of protein allergenicity in addition to the structural similarity.
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