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Establishment of ink jet technology to develop high throughput bio-assay system

Research Project

Project/Area Number 23658138
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionUniversity of Tsukuba (2012-2013)
The University of Tokyo (2011)

Principal Investigator

ENOMAE Toshiharu  筑波大学, 生命環境系, 教授 (40203640)

Co-Investigator(Kenkyū-buntansha) IGARASHI Kiyohiko  東京大学, 農学生命科学研究科, 准教授 (80345181)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsバイオアッセイ / インクジェット / バクテリア / 細胞 / 寒天培地 / 紙 / 培地 / 大腸菌 / ラテックス / アガロース / バイオエタノール
Research Abstract

A novel bioassay system using ink jet printing technology and a paper substrate has been developed. Ink jet printing has potential for high-precision metered dispensing of bacteria and nutrients in various conditions. Paper has the potential to be used as a container to accommodate bacteria and nutrients and is easily disposed of by combustion after use. Filter paper was first immersed in a toluene solution of polystyrene and hydrophobized after drying. Toluene was printed on a few sections of the filter paper to make them hydrophilic again. An agar medium with its viscosity adjusted by acid hydrolysis was printed on the hydrophilic sections. Bacteria were manually dispensed onto the medium, and the colonies of the bacteria were observed to grow for at least 24 h before the medium dried. Poly(vinyl alcohol) with polymerization initiator was also successfully applied as a medium. This bioassay system is expected to be applied for a variety of bacterial and yeast spores, and enzymes.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (52 results)

All 2014 2013 2012 2011 Other

All Journal Article (12 results) (of which Peer Reviewed: 2 results) Presentation (34 results) (of which Invited: 2 results) Book (2 results) Remarks (4 results)

  • [Journal Article] ペーパーエレクトロニクスの現状と課題2014

    • Author(s)
      江前敏晴
    • Journal Title

      日本木材学会誌

      Volume: 60巻4号(印刷中)

    • NAID

      130004678551

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Bacterial Culture System Using a Paper Platform TO Accommodate Media And an Ink-Jet Printing to Dispense Bacteria2014

    • Author(s)
      Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi, Toshiharu Enomae
    • Journal Title

      American Journal of Biochemistry and Biotechnology

      Volume: 10 Issue: 1 Pages: 81-87

    • DOI

      10.3844/ajbbsp.2014.81.87

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Study of Paper Science from Basics —Optical Properties of Paper—2014

    • Author(s)
      江前敏晴
    • Journal Title

      NIHON GAZO GAKKAISHI (Journal of the Imaging Society of Japan)

      Volume: 53 Issue: 1 Pages: 88-93

    • DOI

      10.11370/isj.53.88

    • NAID

      130004484077

    • ISSN
      1344-4425, 1880-4675
    • Related Report
      2013 Final Research Report
  • [Journal Article] 印刷と紙を使ったバイオアッセイ2014

    • Author(s)
      江前敏晴, ティティマナン スリモンコン
    • Journal Title

      日本印刷学会誌

      Volume: 51 Pages: 23-28

    • NAID

      130004513777

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 基礎から学ぶ紙の科学 (V)紙の光学特性2014

    • Author(s)
      江前敏晴
    • Journal Title

      日本画像学会誌

      Volume: 1 Pages: 88-93

    • NAID

      130004484077

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 製紙及び印刷分野におけるグリーンマテリアルの展開2013

    • Author(s)
      江前敏晴
    • Journal Title

      日本接着学会誌

      Volume: Vol.49, No.4 Pages: 141-146

    • NAID

      10031195513

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] 基礎から学ぶ紙の科学 (I)紙の製造技術と構成成分2013

    • Author(s)
      江前敏晴
    • Journal Title

      日本画像学会誌

      Volume: 3 Pages: 229-234

    • NAID

      130004800877

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 基礎から学ぶ紙の科学 (II)紙の構造2013

    • Author(s)
      江前敏晴
    • Journal Title

      日本画像学会誌

      Volume: 4 Pages: 313-317

    • NAID

      130004484051

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 基礎から学ぶ紙の科学 (III)紙と液体の相互作用2013

    • Author(s)
      江前敏晴
    • Journal Title

      日本画像学会誌

      Volume: 5 Pages: 462-466

    • NAID

      130004800887

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 基礎から学ぶ紙の科学 (IV)紙の力学特性2013

    • Author(s)
      江前敏晴
    • Journal Title

      日本画像学会誌

      Volume: 6 Pages: 575-580

    • NAID

      130004484066

    • Related Report
      2013 Annual Research Report
  • [Journal Article] ”印刷ペーパーエレクトロニクス:紙とインクジェットプリンターで作るマイクロ流体ヘルスケアチップ”2013

    • Author(s)
      前島健人, 江前敏晴, 磯貝明, 鈴木孝治, チッテリオ ダニエル
    • Journal Title

      機能紙研究会誌

      Volume: No. 51 Pages: 35-44

    • NAID

      130003380148

    • Related Report
      2012 Research-status Report
  • [Journal Article] Printed Paper Electronics: a Microfluidic Paper-based Healthcare Chip through Inkjet Printing Technology2012

    • Author(s)
      前島健人, 江前敏晴, 磯貝明, 鈴木孝治, チッテリオ ダニエル
    • Journal Title

      Annals of the High Performance Paper Society, Japan

      Volume: 51 Issue: 0 Pages: 35-44

    • DOI

      10.11332/kinoushi.51.35

    • NAID

      130003380148

    • ISSN
      0288-5867, 2187-2740
    • Related Report
      2013 Final Research Report
  • [Presentation] 生物材料グリーンプロセシング研究について2013

    • Author(s)
      江前敏晴, ティティマナン スリモンコン, タンチラ ブンヤピパット, エフィ オクタビア, シュ インチャオ, 望月有希子
    • Organizer
      第52回機能紙研究発表・講演会
    • Place of Presentation
      あわぎんホール(徳島市)
    • Year and Date
      2013-10-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of ink jet printing to paper-based bacterial culture system2013

    • Author(s)
      Tithimanan Srimongkon, Toshiharu Enomae
    • Organizer
      The 4th Asian Symposium on Printing Technology
    • Place of Presentation
      ホーチミンシティ技術教育大学(ベトナム)
    • Year and Date
      2013-09-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Research of paper devices and eco-friendly materials in University of Tsukuba2013

    • Author(s)
      Toshiharu Enomae
    • Organizer
      The 4th Asian Symposium on Printing Technology
    • Place of Presentation
      ホーチミンシティ技術教育大学(ベトナム)
    • Year and Date
      2013-09-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 紙と印刷を利用したグリーンテクノロジーの新しい展開2013

    • Author(s)
      江前敏晴
    • Organizer
      化学工学会第45回秋季大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-09-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Bacterial culture system using paper substrate and ink jet printing2013

    • Author(s)
      Tithimanan Srimongkon, Toshiharu Enomae
    • Organizer
      筑波大学/生物材料グリーンプロセシング研究グループ第1回シンポジウム「パルプ・紙・印刷・バイオマテリアルの明日を開く」
    • Place of Presentation
      筑波大学(つくば市)
    • Year and Date
      2013-09-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of bacterial culture system using paper device for containing media and inkjet printer for dispensing bacteria2013

    • Author(s)
      Tithimanan Srimongkon, Toshiharu Enomae
    • Organizer
      第80回紙パルプ研究発表会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2013-06-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of inkjet technology to bacterial culture system by using paper devices2013

    • Author(s)
      Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi, Toshiharu Enomae
    • Organizer
      第63回日本木材学会大会
    • Place of Presentation
      岩手大学(岩手県)
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] インクジェット印刷技術を用いた紙基板健康診断チップの開発2013

    • Author(s)
      前島健人, 磯貝明, 江前敏晴
    • Organizer
      第63回日本木材学会大会
    • Place of Presentation
      岩手大学(岩手県)
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel paper-based applications built on fundamental paper technology in Japan2012

    • Author(s)
      Toshiharu Enomae, Tithimanan Srimongkon, Tunchira Bunyaphiphat, Kento Maejima
    • Organizer
      2012 International Symposium ON RESOURCE EFFICIENCY IN PULP AND PAPER TECHNOLOGY
    • Place of Presentation
      Bandung (Indonesia)
    • Year and Date
      2012-11-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Paper-based tools with fluidic channels patterned by ink jet printing2012

    • Author(s)
      Toshiharu Enomae, Tithimanan Srimongkon, Kento Maejima, Takuya Ishida, Kiyohiko Igarashi
    • Organizer
      The 4th International Symposium of IWoRS
    • Place of Presentation
      Makassar (INDONESIA)
    • Year and Date
      2012-11-07
    • Related Report
      2013 Final Research Report
  • [Presentation] エマルション粒子及びバクテリアのインクジェットパターニング2012

    • Author(s)
      スリモンコン ティティマナン, 江前敏晴, 石田卓也, 五十嵐圭日子
    • Organizer
      平成24年度繊維学会秋季研究発表会
    • Place of Presentation
      福井大学(福井県)
    • Year and Date
      2012-09-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Ink-jet printing technology for stable arrangement of bacterial cells2012

    • Author(s)
      Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi, Toshiharu Enomae
    • Organizer
      The 3rd Asian Symposium on Printing Technology
    • Place of Presentation
      Bangkok (Thailand)
    • Year and Date
      2012-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] All Inkjet Printed "Lab-on-Paper" with Electrodes and Microchannels2012

    • Author(s)
      Kento Maejima, Toshiharu Enomae, Akira Isogai, Koji Suzuki, Daniel Citterio
    • Organizer
      DIGITALFABRICATION 2012, IS&T
    • Place of Presentation
      Quebec (Canada)
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Ink jet printing with emulsified particulates and bacterial cells aiming at electrochemical sensing device made of paper2012

    • Author(s)
      Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi, Toshiharu Enomae
    • Organizer
      2012 International Conference on Flexible and Printed Electronics
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2012-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 印刷エレクトロニクス技術によるペーパーキャピラリーセンサーの開発2012

    • Author(s)
      前島健人, 江前敏晴, 磯貝明, 鈴木 孝治, チッテリオ ダニエル
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県)
    • Year and Date
      2012-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 印刷エレクトロニクス技術によるペーパーキャピラリーセンサーの開発2012

    • Author(s)
      前島健人, 江前敏晴, 磯貝明, 鈴木 孝治, チッテリオ ダニエル
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] All-inkjet-printed fabrication method for "paperfluidic" colorimetric & electrochemical sensing devices2011

    • Author(s)
      Kento Maejima, Toshiharu Enomae, Akira Isogai, Koji Suzuki, Daniel Citterio
    • Organizer
      The 2011 Asian Symposium on Printing Technology (2011ASPT)
    • Place of Presentation
      東京ビッグサイト(東京都)
    • Year and Date
      2011-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ろ紙を基板とした健康診断電極チップの全工程インクジェット印刷による作製2011

    • Author(s)
      前島健人, 江前敏晴, 磯貝明, 鈴木 孝治, チッテリオ ダニエル
    • Organizer
      第50回機能紙研究発表講演会
    • Place of Presentation
      高知県立県民文化ホール(高知県)
    • Year and Date
      2011-01-27
    • Related Report
      2013 Final Research Report
  • [Presentation] ろ紙を基板とした健康診断電極チップの全工程インクジェット印刷による作製2011

    • Author(s)
      前島健人, 江前敏晴, 磯貝明, 鈴木 孝治, チッテリオ ダニエル
    • Organizer
      第50回機能紙研究発表講演会
    • Place of Presentation
      高知県立県民文化ホール(高知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] All-inkjet-printed fabrication method for "paperfluidic" colorimetric & electrochemical sensing devices2011

    • Author(s)
      Kento Maejima, Toshiharu Enomae, Akira Isogai, Koji Suzuki, Daniel Citterio
    • Organizer
      The 2011Asian Symposium on Printing Technology (2011ASPT)
    • Place of Presentation
      東京ビッグサイト(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Bacterial culture system using paper substrate and ink jet printing

    • Author(s)
      Tithimanan Srimongkon, Toshiharu Enomae
    • Organizer
      筑波大学/生物材料グリーンプロセシング研究グループ 第1 回シンポジウム「パルプ・紙・印刷・バイオマテリアルの明日を開く」
    • Place of Presentation
      筑波大学大学会館(つくば市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of bacterial culture system using paper device for containing media and inkjet printer for dispensing bacteria

    • Author(s)
      Tithimanan Srimongkon, Toshiharu Enomae
    • Organizer
      第80回紙パルプ研究発表会
    • Place of Presentation
      東京大学弥生講堂(東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Application of ink jet printing to paper-based bacterial culture system

    • Author(s)
      Tithimanan Srimongkon, Toshiharu Enomae
    • Organizer
      The 4th Asian Symposium on Printing Technology 2013
    • Place of Presentation
      ホーチミンシティ技術教育大学(ベトナム)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 紙と印刷を利用したグリーンテクノロジーの新しい展開

    • Author(s)
      江前敏晴
    • Organizer
      化学工学会 第45回秋季大会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Research of paper devices and eco-friendly materials in University of Tsukuba

    • Author(s)
      Toshiharu Enomae
    • Organizer
      The 4th Asian Symposium on Printing Technology 2013
    • Place of Presentation
      ホーチミンシティ技術教育大学(ベトナム)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生物材料グリーンプロセシング研究について

    • Author(s)
      江前敏晴, ティティマナン スリモンコン, タンチラ ブンヤピパット, エフィ オクタビア, シュ インチャオ, 望月有希子
    • Organizer
      第52回機能紙研究発表・講演会
    • Place of Presentation
      あわぎんホール(徳島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 紙のコート層の微細構造と印刷適性

    • Author(s)
      江前敏晴
    • Organizer
      印刷インキアドバンス講座―印刷インキ及び関連分野の最新技術動向
    • Place of Presentation
      東京塗料会館(東京)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] インクジェット印刷技術を用いた紙基板健康診断チップの開発

    • Author(s)
      前島健人, 磯貝明, 江前敏晴
    • Organizer
      第63回日本木材学会大会
    • Place of Presentation
      岩手大学(岩手県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Novel paper-based applications built on fundamental paper technology in Japan

    • Author(s)
      Toshiharu Enomae, Tithimanan Srimongkon, Tunchira Bunyaphiphata, Kento Maejima
    • Organizer
      2012 International Symposium ON RESOURCE EFFICIENCY IN PULP AND PAPER TECHNOLOGY
    • Place of Presentation
      Bandung, Indonesia
    • Related Report
      2012 Research-status Report
  • [Presentation] Paper-based tools with fluidic channels patterned by ink jet printing

    • Author(s)
      Toshiharu Enomae, Tithimanan Srimongkon, Kento Maejima, Takuya Ishida, Kiyohiko Igarashi
    • Organizer
      The 4th International Symposium of IWoRS
    • Place of Presentation
      Makassar, INDONESIA
    • Related Report
      2012 Research-status Report
  • [Presentation] エマルション粒子及びバクテリアのインクジェットパターニング

    • Author(s)
      スリモンコン ティティマナン, 江前敏晴, 石田卓也 五十嵐圭日子
    • Organizer
      平成24年度繊維学会秋季研究発表会
    • Place of Presentation
      福井大学(福井県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Ink-jet printing technology for stable arrangement of bacteria cells

    • Author(s)
      Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi, Toshiharu Enomae
    • Organizer
      The 3rd Asian Symposium on Printing Technology
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2012 Research-status Report
  • [Presentation] All Inkjet Printed “Lab-on-Paper”with Electrodes and Microchannels

    • Author(s)
      Kento Maejima,Toshiharu Enomae,Akira Isogai, Koji Suzuki, Daniel Citterio
    • Organizer
      DIGITALFABRICATION 2012, IS&T
    • Place of Presentation
      Quebec, Canada
    • Related Report
      2012 Research-status Report
  • [Presentation] Ink jet printing with emulsified particulates and bacterial cells aiming at electrochemical sensing device made of paper

    • Author(s)
      Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi, Toshiharu Enomae
    • Organizer
      2012 International Conference on Flexible and Printed Electronics
    • Place of Presentation
      The Univ of Tokyo, Tokyo
    • Related Report
      2012 Research-status Report
  • [Book] 分散・塗布・乾燥の基礎と応用 第1 編基礎編 第4章欠陥 第2節乾燥欠陥 7.偏析2014

    • Author(s)
      江前敏晴
    • Total Pages
      600
    • Publisher
      株式会社テクノシステム
    • Related Report
      2013 Final Research Report
  • [Book] 分散・塗布・乾燥の基礎と応用 第1編 基礎編 第4章 欠陥 第2節 乾燥欠陥 7.偏析2014

    • Author(s)
      江前敏晴、山口由岐夫、他42名
    • Total Pages
      600
    • Publisher
      テクノシステム
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.enomae.com/

    • Related Report
      2013 Final Research Report
  • [Remarks] 江前敏晴のホームページ

    • URL

      http://www.enomae.com/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 江前敏晴のホームページ

    • URL

      http://www.enomae.com/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.enomae.com/

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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