| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The economical values of some fish species are much different between male and female. Identification of sex-related DNA sequences is an important key step to enable sex-selected breeding of these fish species.
This study developed a method to identify sex-related DNA sequences using next generation sequencer in Nile tilapia as an experimental fish model. In this study, male-related DNA sequences were isolated in the tilapia, which enable to identify 90% of genetic males. Using these sequences as DNA markers makes much easier in production of mono-sex population in the tilapia.
In sturgeons, we failed to identify sex-related sequences using whole RNA sequence method in this study. We expect that future screening utilizing combination of DNA and RNA deep sequencing, which was developed in the tilapia study, would identify sex-related DNA sequences which enable to identify genetic sex in the sturgeons. This would help to develop female-selected aquaculture in sturgeons.
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