Development of a method to identify sex-related DNA sequences to enable production of mono-sex population in fish

• Project/Area Number: 23658153
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: IJIRI Shigeho 北海道大学, 水産科学研究科(研究院), 准教授 (90425421)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ティラピア / 性統御

Outline of Final Research Achievements

The economical values of some fish species are much different between male and female. Identification of sex-related DNA sequences is an important key step to enable sex-selected breeding of these fish species.
This study developed a method to identify sex-related DNA sequences using next generation sequencer in Nile tilapia as an experimental fish model. In this study, male-related DNA sequences were isolated in the tilapia, which enable to identify 90% of genetic males. Using these sequences as DNA markers makes much easier in production of mono-sex population in the tilapia.
In sturgeons, we failed to identify sex-related sequences using whole RNA sequence method in this study. We expect that future screening utilizing combination of DNA and RNA deep sequencing, which was developed in the tilapia study, would identify sex-related DNA sequences which enable to identify genetic sex in the sturgeons. This would help to develop female-selected aquaculture in sturgeons.

Research Products

• [Journal Article] Expression patterns of gonadotropin hormones and their receptors during early sexual differentiation in Nile tilapia Oreochromis niloticus (2012)
  • Author(s): Yan, H., Ijiri, S., Wu, Q., Kobayashi, T., Li, S., Nakaseko, T., Adachi, S. and Nagahama, Y
  • Journal Title: Biol. Reprod Volume: 87 Issue: 5 Pages: 116-126
  • DOI: 10.1095/biolreprod.112.101220
  • Peer Reviewed
• [Presentation] Expression patterns of gonadotropin hormones and their receptors during early sexual differentiation
  • Author(s): Hongwei Yan, Shigeho Ijiri, Quan Wu, Tohru Kobayashi, Shuang Li, Taro Nakaseko, Shinji Adachi, Yoshitaka Nagahama
  • Organizer: 7th INTERNATIONAL SYMPOSIUM ON FISH ENDOCRINOLOGY
  • Place of Presentation: NH City Hotel (アルゼンチン・ブエノスアイレス)
• [Presentation] ナイルティラピア遺伝的雄仔魚の雌化誘導に伴う性分化関連遺伝子群の発現変動
  • Author(s): 李爽・井尻成保・ヤンホンウェイ・西澤朋実・足立伸次・長濱嘉孝
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
• [Presentation] Roles of GTHs in gonadal sex differentiation of Nile tilapia
  • Author(s): Hongwei Yan・Shigeho Ijiri・Shuang Li・Shinji Adachi・Tohru Kobayashi・Yoshitaka Nagahama
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
• [Presentation] ティラピア性決定・性分化期における濾胞刺激ホルモンの発現
  • Author(s): ヤン紅偉・中世古太郎・呉泉・井尻成保・足立伸次（北大院水）
  • Organizer: 日本水産学会秋期大会
  • Place of Presentation: 長崎大学文教キャンパス
• [Presentation] ナイルティラピア遺伝的雌仔魚の雄化に伴う性分化関連遺伝子群の発現変動
  • Author(s): 李爽・吉田沙織・倉持有希・井尻成保・足立伸次 (北大院水)
  • Organizer: 日本水産学会秋期大会
  • Place of Presentation: 長崎大学文教キャンパス
• [Presentation] ナイルティラピア黄体形成ホルモンの発現解析
  • Author(s): 李爽・ヤン紅偉・中世古太郎・井尻成保・足立伸次（北大院水）
  • Organizer: 日本水産学会北海道支部大会
  • Place of Presentation: 北海道大学水産学部