| Project/Area Number |
23658159
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Mie University |
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Principal Investigator |
YOSHIOKA Motoi 三重大学, 大学院・生物資源学研究科, 教授 (30262992)
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| Co-Investigator(Kenkyū-buntansha) |
ASAKAWA Shuichi 東京大学, 大学院・農学生命科学研究科, 教授 (30231872)
TAKAYANAGI Atsushi 慶應義塾大学, 医学部, 講師 (80245464)
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| Co-Investigator(Renkei-kenkyūsha) |
KATO Hidehiro 東京海洋大学, 海洋科学部, 教授 (30371917)
KOIZUMI Kenji 順天堂大学, 医学部, 助教 (40053342)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 遺伝・育種 / イルカ / 形態 / 後肢 / ゲノム解析 / 不死化 / iPS細胞 / Tursiops / ホメオボックス遺伝子 |
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| Research Abstract |
In order to elucidate the genetic mechanism of the hind limb formation of the unique female bottlenose dolphin (Tursiops truncatus) with fin-shaped hind limbs, we performed genome analysis of the dolphin using a next generation sequencer. We found a missense mutation in a homeobox gene, which is known to regulate development and formation of the hind limbs in mammals, therefore the gene is a strong candidate responsible for the hind limb formation in the dolphin. For the DNA source and future developmental biological experiments of her genome, we are trying to establish her immortal cell lines and iPS cells by recombinant viruses
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