Molecular mechanism of site-specificity of Caligus fugu on fins of puffer fish

• Project/Area Number: 23658162
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Hiroshima University
• Principal Investigator: OHTSUKA Susumu 広島大学, 生物圏科学研究科, 教授 (00176934)
• Co-Investigator(Kenkyū-buntansha): TASUMI Satoshi 東京大学, 大学院・農学生命科学研究科, 特任助教 (90359646) ; SUZUKI Yuzuru 東京大学, 大学院・農学生命科学研究科, 教授 (40107412)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: トラフグ類 / ウオジラミ類 / 宿主特異性 / 寄生部位特異性 / 鰭 / サブトラクティブPCR法 / カワハギ類 / ペンネラ科 / 寄生 / サブトラクティブハイブリダイゼーション法 / ペンネラ類 / 国際情報交換 / 「国際情報交換」（連合王国） / トラフグ / PCR / 生活史 / 感染 / Pseudocaligus fugu / copepodid / フグ / 細胞表面ディスプレイ法 / レセプター / リガンド

Research Abstract

(1) The life cycle and developmental stages of some parasitic copepods have been newly revealed by the present investigation. The number of post-naupliar stages of caligids is evidently six. Reinfection of adults is implied in some caligids. The whole life cycle of a pennelid Peniculus minuticaudae infecting fins of filefish is totally disclosed: the hatching stage is copepodids; no host switching is found. It is likely that fish fins are feasibly infected by infective stages of parasitic copepods but so limited for fecundity of adult females. (2) Results of bioassay indicate that attractants for the infective copepodid of Caligus fugu is water-soluble. At least 10 genes coding secretory proteins or enzymes were obtained by suppression subtractive hybridization, which was applied to isolate genes expressed higher in fins compared to skin. These are likely to be candidate genes related to the attachment site-specificity of the infective stage.

Research Products

• [Journal Article] The caligid life cycle: new evidence from Leophtheirus elegans reconciles the cycles of Caligus and Lepeophtheirus (Copepodoa : Caligidae) (2013)
  • Author(s): B. A. Venmathi Maran, S. Y. Moon, S. Ohtsuka, S.-Y. Oh, H. Y. Soh, J.-G. Myoung, A. Iglikowska, G. A. Boxshall
  • Journal Title: Parasite Volume: 20, 15 Pages: 1-22
  • Peer Reviewed
• [Journal Article] Complete life cycle of a pennellid Peniculus minuticaudae Shiino, 1956 (Copepoda : Siphonostomatoida) infecting cultured threadsail filefish, Stephanolepis cirrhifer (2013)
  • Author(s): N. Ismail, S. Ohtsuka, B. A. Venmathi Maran, S. Tasumi, K. Zeleha, H. Yamashita
  • Journal Title: Parasite Volume: 20, 42 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Complete life cycle of a pennellid Peniculus minuticaudae Shiino, 1956 (Copepoda: Siphonostomatoida) infecting cultured threadsail filefish, Stephanolepis cirrhifer (2013)
  • Author(s): Norshida Ismail, Susumu Ohtsuka, Balu Alagar Venmathi Maran, Satoshi Tasumi, Kassim Zeleha, Hirofumi Yamashita
  • Journal Title: Parasite Volume: 2013,20,42 Pages: 1-16
  • DOI: 10.1051/parasite/2013041
  • Peer Reviewed
• [Journal Article] The caligid life cycle: new evidence from Lepeophtheirus elegans reconciles the cycles of Caligus and Lepeophtheirus (Copepoda: Caligidae) (2013)
  • Author(s): Balu Alagar Venmathi Maran, Seong Yong Moon, Susumu Ohtsuka, Sung-Yong Oh, Ho Young Soh, Jung-Goo Myoung, Anna Iglikowska, Geoffrey Allan Boxshall
  • Journal Title: Parasite Volume: 2013,20,15 Pages: 1-22
  • Peer Reviewed
• [Journal Article] The caligid life cycle: new evidence from Lepeophtheiurs elegans reconciles the cycles of Caligus and Lepeophtheirus (Copepoda: Caligidae) (2013)
  • Author(s): B.A. Venmathi Maran, S. Y. Moon, S. Ohtsuka, S. Y. Oh, H. Y. Soh, J.-G. Myoung, A. Iglikowska & G. A. Boxshall
  • Journal Title: Parasite Volume: 20 Pages: 15-15
  • DOI: 10.1051/parasite/2013015
  • Peer Reviewed
• [Journal Article] Occurrene of caligid copepods (Crustacea) in plankton samples collected from Japan and Thailand, with the description of a new species (2012)
  • Author(s): B. A. Venmathi Maran, S. Ohtsuka, P. Jitchum
  • Journal Title: Species Diversity Volume: 17 Pages: 87-95
  • Peer Reviewed
• [Journal Article] Records of adult caligiform copepods (Crustacea : Copepoda: Siphonostomatoida) in marine plankton from East Asia, including descriptions of two new species of Caligus (Caligidae) (2012)
  • Author(s): B. A. Venmathi Maran, S. Ohtsuka, X. Shang
  • Journal Title: Species Diversity Volume: 17 Pages: 201-219
  • NAID: 110009561662
  • Peer Reviewed
• [Journal Article] Records of adult caligiform copepods (Crustacea: Copepoda: Siphonostomatoida) in marine plankton from East Asia, including descriptions of two new species of Caligus (Caligidae) (2012)
  • Author(s): B. A. Venmathi Maran, S. Ohtsuka & Xu Shang
  • Journal Title: Species Diversity Volume: 17 Pages: 201-219
  • NAID: 110009561662
  • Peer Reviewed
• [Presentation] トラフグの鰭と皮膚において発現量の異なる遺伝子単離の試みー寄生虫防御の観点からー (2014)
  • Author(s): 田角聡志, ノシダビンチイイスマイル, 鈴木譲, 菊池潔, 大塚攻
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 函館市
  • Year and Date: 2014-03-28
• [Presentation] カワハギに寄生するカイアシ類 Peniculus minuticaudaeの生活史 (2014)
  • Author(s): Norshida Ismail, 大塚攻, B.A. Venmathi Maran, 田角聡志, 山下浩史
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 函館市
  • Year and Date: 2014-03-28
• [Presentation] トラフグとクサフグの鰓において発現量の異なる遺伝子単離の試み (2012)
  • Author(s): 田角聡志・菊池潔・鈴木譲
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 下関市
  • Year and Date: 2012-09-15
• [Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes (2012)
  • Author(s): S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
  • Organizer: The 12th Congress of ISDCI
  • Place of Presentation: 福岡市
  • Year and Date: 2012-07-10
• [Presentation] トラフグのゲノム育種 (2011)
  • Author(s): 鈴木譲・菊池潔
  • Organizer: 平成23年度日本水産学会秋季大会, 日本水産学会シンポジウム「フグ研究 とトラフグ生産技術開発の最前線」
  • Place of Presentation: 長崎市
  • Year and Date: 2011-10-02
  • Invited
• [Presentation] 寄生虫の宿主特異性に関わるフグ遺伝子の探索 (2011)
  • Author(s): 平林陽・木戸慎一・木南竜平・細谷将・甲斐渉・菊池潔・城夕香・末武弘章・良永知義・小川和夫・鈴木譲
  • Organizer: 日本比較免疫学会第23回学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-08-22
• [Presentation] トラフグの鰭と皮膚において発現量の異なる遺伝子単離の試みー寄生虫防御の観点からー
  • Author(s): 田角 聡志、ノシダビンチイイスマイル、鈴木 譲、菊池 潔、大塚 攻
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 函館市
• [Presentation] カワハギに寄生するカイアシ類 Peniculus minuticaudaeの生活史
  • Author(s): Norshida Ismail、大塚 攻、B.A. Venmathi Maran、田角 聡志、山下 浩史
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 函館市
• [Presentation] Candidate key molecule(s) determining host specificity of parasite on fugu, Takifugu rubripes
  • Author(s): S. Tasumi, A. Yamaguchi, Y. Hirabayashi, S. Kido, K. Kobayashi, W. Kai, S. Hosoya, S. Tsutsui, O. Nakamura, H. Suetake, K. Kikuchi, Y. Suzuki
  • Organizer: The 12th Congress of ISDCI
  • Place of Presentation: 福岡市
• [Presentation] 寄生虫の宿主特異性に関わるフグ遺伝子の探索
  • Author(s): 平林陽・木戸慎一・木南竜平・細谷将・甲斐渉・菊池潔・城夕香・末武弘章・良永知義・小川和夫・鈴木譲
  • Organizer: 日本比較免疫学会第23回学術集会
  • Place of Presentation: 長崎
• [Presentation] トラフグのゲノム育種
  • Author(s): 鈴木譲・菊池潔
  • Organizer: 平成23年度日本水産学会秋季大会、日本水産学会シンポジウム「フグ研究 とトラフグ生産技術開発の最前線」(招待講演)
  • Place of Presentation: 長崎
• [Remarks] 広島大学大学院生物圏科学研究科附属瀬戸内圏フィールド科学教育研究センター竹原ステーション(水産実験所)
  • URL: http://fishlab.hiroshima-u.ac.jp/index.html
• [Remarks] 東京大学大学院農学生命科学研究科附属水産実験所
  • URL: http://www.se.a.u-tokyo.ac.jp/about_us.html
• [Remarks] 広島大学大学院生物圏科学研究科附属瀬戸内圏フィールド科学教育研究センター竹原ステーション
  • URL: http://fishlab.hiroshima-u.ac.jp/
• [Remarks] 東京大学大学院農学生命科学研究科附属水産実験所
  • URL: http://www.se.a.u-tokyo.ac.jp/about_us.html
• [Remarks] 竹原ステーションにおける展開中の研究