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Pearl production by transplantation of outer epithelial cells of mantle and a novel technology to control yellowness of pearls.

Research Project

Project/Area Number 23658169
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General fisheries
Research InstitutionFisheries Research Agency

Principal Investigator

AWAJI Masahiko  独立行政法人水産総合研究センター, その他部局等, その他 (20371825)

Co-Investigator(Kenkyū-buntansha) KAKINUMA Makoto  三重大学, 生物資源学研究科, 准教授 (60303757)
Co-Investigator(Renkei-kenkyūsha) WATABE Shugo  北里大学, 海洋生命科学部 (40111489)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords真珠 / 細胞培養 / 色素 / アコヤガイ / 外套膜
Research Abstract

In pearl culture, a small piece of mantle tissue is implanted into a gonad of a pearl oyster in adjacent to a shell bead that is implanted concomitantly. Then outer epithelial cells of the mantle fragment start to migrate along the surface of the bead, which leads to the formation of follicular structure surrounding the bead and subsequent formation of pearl layers on the sureface of the bead. In this study, techniques for pearl formation by the transplantation of the outer epithelial cells were developped. Moreover, transplantation of the blended outer epithelial cells which produce pearls with different yellowness enabled control of the yellowness of the pearls. These results imply future possibilities of pearl production by blending of different types of outer epithelial cells that can produce pearls with differing superior qualities.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 4 results) Presentation (14 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] アコヤガイ外套膜から分離した外面上皮細胞の移植による真珠形成2014

    • Author(s)
      淡路雅彦・山本貴志・柿沼 誠・永井清仁・渡部終五
    • Journal Title

      日本水産学会誌

      Volume: 80(印刷中)

    • NAID

      130004678121

    • Related Report
      2013 Final Research Report
  • [Journal Article] シンポジウム記録 外套膜外面上皮細胞の移植による真珠形成2014

    • Author(s)
      淡路雅彦
    • Journal Title

      日本水産学会誌

      Volume: 80 Pages: 110-110

    • Related Report
      2013 Final Research Report
  • [Journal Article] シンポジウム記録 黄色系アコヤガイ真珠の色素とその特性2014

    • Author(s)
      柿沼 誠
    • Journal Title

      日本水産学会誌

      Volume: 80 Pages: 108-108

    • Related Report
      2013 Final Research Report
  • [Journal Article] シンポジウム記録 外套膜外面上皮細胞の移植による真珠形成2014

    • Author(s)
      淡路雅彦
    • Journal Title

      日本水産学会誌

      Volume: 80

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シンポジウム記録 黄色系アコヤガイ真珠の色素とその特性2014

    • Author(s)
      柿沼誠
    • Journal Title

      日本水産学会誌

      Volume: 80 Pages: 108-108

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アコヤガイ外套膜から分離した外面上皮細胞の移植による真珠形成2014

    • Author(s)
      淡路雅彦、山本貴志、柿沼誠、永井清仁、渡部終五
    • Journal Title

      日本水産学会誌

      Volume: 80

    • NAID

      130004678121

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fundamental studies on in vivo and in vitro pearl formation- Contribution of outer epithelial cells of pearl oyster mantle and pearl sacs2011

    • Author(s)
      Masahiko Awaji ・Akira Machii
    • Journal Title

      Aqua-Bioscience Monographs

      Volume: 4 Issue: 1 Pages: 1-39

    • DOI

      10.5047/absm.2011.00401.0001

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] アコヤガイ外套膜外面上皮細胞が作る濾胞状構造の形態とその形成に培養液が及ぼす影響2013

    • Author(s)
      淡路雅彦
    • Organizer
      第8回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京大学
    • Year and Date
      2013-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 外套膜外面上皮細胞の移植による真珠形成((シンポジウム・真珠研究の最前線-真珠養殖技術の革新を目指して-)2013

    • Author(s)
      淡路雅彦
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 黄色系アコヤガイ真珠の色素とその性状(シンポジウム・真珠研究の最前線-真珠養殖技術の革新を目指して-)2013

    • Author(s)
      柿沼 誠
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2013-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] アコヤガイ貝殻真珠層の黄色色素の性状と構造解析2013

    • Author(s)
      三谷花代・ 前山 薫・服部文弘・永井清仁・淡路雅彦・渡部終五・幹 渉・柿沼誠
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] アコヤガイ外套膜外面上皮細胞の移植による真珠形成法の検討2013

    • Author(s)
      淡路雅彦・山本貴志・永井清仁・柿沼 誠
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Primary culture of the outer epithelial cells of pearl oyster mantle2011

    • Author(s)
      Masahiko Awaji
    • Organizer
      The International Symposium on Pearl Research
    • Place of Presentation
      東京大学
    • Year and Date
      2011-10-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Extraction and characterization of a yellow pigment in the nacre of pearl oyster shells2011

    • Author(s)
      Kayo Mitani・Kaoru Maeyama・Fumihiro Hattori・Kiyohito Nagai・Shugo Watabe・Wataru Miki ・Makoto Kakinuma
    • Organizer
      The International Symposium on Pearl Research
    • Place of Presentation
      東京大学
    • Year and Date
      2011-10-05
    • Related Report
      2013 Final Research Report
  • [Presentation] アコヤガイ貝殻真珠層の黄色色素の性状2011

    • Author(s)
      三谷花代・前山 薫・服部文弘・永井清仁・渡部終五・幹 渉・柿沼 誠
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学
    • Year and Date
      2011-09-29
    • Related Report
      2013 Final Research Report
  • [Presentation] アコヤガイ外套膜外面上皮細胞が作る濾胞状構造の形態とその形成に培養液が及ぼす影響

    • Author(s)
      淡路雅彦
    • Organizer
      第8回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京大学理学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] 外套膜外面上皮細胞の移植による真珠形成

    • Author(s)
      淡路雅彦
    • Organizer
      平成25年度日本水産学会秋季大会シンポジウム
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 黄色系アコヤガイ真珠の色素とその特性

    • Author(s)
      柿沼誠
    • Organizer
      平成25年度日本水産学会秋季大会シンポジウム
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] アコヤガイ外套膜外面上皮細胞の移植による真珠形成法の検討

    • Author(s)
      淡路雅彦、山本貴志、永井清仁、柿沼誠
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] アコヤガイ貝殻真珠層の黄色色素の性状と構造解析

    • Author(s)
      三谷花代、前山薫、服部文弘、永井清仁、淡路雅彦、渡部終五、幹渉、柿沼誠
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Primary culture of the outer epithelial cells of pearl oyster mantle.

    • Author(s)
      Masahiko Awaji
    • Organizer
      The international symposium on pearl research.(招待講演)
    • Place of Presentation
      東京大学弥生講堂
    • Related Report
      2011 Research-status Report
  • [Book] The outer epithelial cells of pearl oyster mantle - Morphology, functions, and primary culture methods. in "Recent Advances in Pearl Research"2013

    • Author(s)
      Masahiko Awaji
    • Total Pages
      12
    • Publisher
      TERRAPUB, Tokyo
    • Related Report
      2012 Research-status Report
  • [Book] The outer epithelial cells of pearl oyster mantle - Morphology, functions, and primary culture methods "Recent Advances in Pearl Research"2012

    • Author(s)
      Masahiko Awaji(Watabe S, Maeyama K, Nagasawa H eds)
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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