Elucidation of the tetrodotoxin biosynthesis pathway by whole genome analysis of tetrodotoxin producing bacteria

• Project/Area Number: 23658173
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fisheries chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: WATABE Shugo 東京大学, 大学院・農学生命科学研究科, 教授 (40111489)
• Co-Investigator(Renkei-kenkyūsha): ASAKAWA Shuichi 東京大学, 大学院・農学生命科学研究科, 教授 (30231872)
• Research Collaborator: MATSUMOTO Takuya , 日本学術振興会・特別研究員・PD ; KIKUCHI Ryosuke 東京大学, 大学院・農学生命科学研究科, 修士課程 ; HOLGER Feroudj 東京大学, 大学院・農学生命科学研究科, 修士課程
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: フグ毒 / テトロドトキシン / フグ毒産生菌 / Vibrio alginolyticus / 生合成経路 / 全ゲノム / 次世代シーケンス

Research Abstract

The purpose of this study was to elucidate the biosynthesis pathway of pufferfish toxin, tetrodotoxin(TTX), in TTX-producing marine bacterium Vibrio alginolyticus by the whole genome analysis. We identified 6 strains of V. alginolyticus from intestinal flora of pufferfish and TTX was detected from 2 strains by MALDI-TOF-MS analysis. Genomic DNA samples extracted from the TTX-producing V. alginolyticus strains were subjected to whole genome analysis by the next-generation sequencing technology.

Research Products

• [Presentation] Expression analysis of genes related to tetrodotoxin pharmacokinetics in ovary tissues of wild pufferfish Takifugu rubripes (2011)
  • Author(s): H. Feroudj, T. Matsumoto, R. Kikuchi, Y. Kawana, H. Kondo, I. Hirono, S. Aki, T. Mochizuki, Y. Nagashima, G. Kaneko, H. Ushio, M. Kodama, S. Watabe
  • Organizer: The international symposium on muscle biochemistry
  • Place of Presentation: 東京大学農学部(東京都文京区)
  • Year and Date: 2011-10-28
• [Presentation] Microarray analysis on the liver of cultured marine pufferfish Takifugu rubripes after the intramuscular administration of tetrodotoxin (2011)
  • Author(s): T. Matsumoto, H. Feroudj, R. Kikuchi, Y. Kawana, H. Kondo, I. Hirono, S. Aki, T. Mochizuki, Y. Nagashima, G. Kaneko, H. Ushio, M. Kodama, S. Watabe
  • Organizer: The international symposium on muscle biochemistry
  • Place of Presentation: 東京大学農学部(東京都文京区)
  • Year and Date: 2011-10-28
• [Presentation] Suppression subtractive hybridization analysis in the skin of cultured marine pufferfish Takifugu snyderi after the intramuscular administration of tetrodotoxin (2011)
  • Author(s): R. Kikuchi, T. Matsumoto, H. Feroudj, H. Doi, T. Ishibashi, Y. Nagashima, G. Kaneko, H. Ushio, M. Kodama, S. Watabe
  • Organizer: The international symposium on muscle biochemistry
  • Place of Presentation: 東京大学農学部(東京都文京区)
  • Year and Date: 2011-10-28
• [Presentation] III-3フグ毒蓄積関連遺伝子 (2011)
  • Author(s): 渡部終五,松本拓也
  • Organizer: 平成23年度日本水産学会秋季大会シンポジウム企画委員会主催シンポジウム「フグ研究とトラフグ生産技術開発の最前線」
  • Place of Presentation: 長崎大学水産学部(長崎県長崎市)
  • Year and Date: 2011-10-02
• [Presentation] フグ類皮膚におけるテトロドトキシン関連遺伝子の発現解析とTTX腸内産生菌の単離の試み (2011)
  • Author(s): 菊地遼輔,松本拓也, Holger Feroudj,土井啓行,石橋敏章,長島裕二,金子元,潮秀樹,児玉正昭,渡部終五
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学水産学部(長崎県長崎市)
  • Year and Date: 2011-09-29
• [Presentation] Microarray analysis of tetrodotoxin accumulation-related genes in the ovary of wild pufferfish Takifugu rubripes (2011)
  • Author(s): Feroudj Holger,松本拓也,菊地遼輔,川名由利子,近藤秀裕,廣野育生,安藝真一郎,望月俊孝,長島裕二,金子元,潮秀樹,児玉正昭,渡部終五
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学水産学部(長崎県長崎市)
  • Year and Date: 2011-09-29
• [Presentation] フグ類皮膚におけるテトロドトキシン関連遺伝子の発現解析とTTX腸内産生菌の単離の試み (2011)
  • Author(s): 菊地遼輔
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学文京キャンパス
  • Year and Date: 2011-09-29
• [Book] 日本水産学会監修,水産学シリーズ第174号「トラフグの生産技術開発と安全確保(仮題)」 (2012)
  • Author(s): 松本拓也,渡部終五
  • Publisher: 恒星社厚生閣
• [Remarks]
  • URL: http://mbl.fs.a.u-tokyo.ac.jp/index.html