Visualization of expression of gene increased the unsaturated fatty acids in meat

• Project/Area Number: 23658217
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Zootechnical science/Grassland science
• Research Institution: Tohoku University
• Principal Investigator: KATOH Kazuo 東北大学, (連合)農学研究科(研究院), 教授 (60091831)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 食肉組成 / 不飽和脂肪酸 / SCD / 遺伝子導入 / 可視化

Research Abstract

This study was to 1) investigate the gene expression of SCD in bovine adipocytes, and 2) generate delta-9-desaturease-GFP- knock in mice to visualize the activation of an enzyme generating a flavor and taste of the intramuscular fat.
1) Bovine GH (100 and 1000 ng/ml) decreased SCD mRNA expression in bovine differentiated adipocytes. 2) Using the homologous recombination method, embryo stem cell derived from the mouse embryo with GFP protein in an Exon1 of the internal delta-9 desaturase was established. The chimera mouse with delta-9-desaturease-GFP was identified and hetero delta-9-desaturease-GFP mouse generated.

Research Products

• [Journal Article] 2014. The effect of single nucleotide polymorphisms in GH gene promoter region on carcass traits and intramuscular fatty acid compositions in Japanese Black cattle (2014)
  • Author(s): Sugita H, Ardiyanti A, Yokota S, Yonekura S, Hirayama H, Shoji N, Yamauchi E, Suzuki K, Katoh K, Roh SG (Corresponding author)
  • Journal Title: Livestock Science Volume: (Accepted)
  • Peer Reviewed
• [Journal Article] 2014. Actions of intravenous injections of AVP and oxytocin on plasma ACTH, GH, insulin, and glucagon concentrations in goats (2014)
  • Author(s): Roh SG, Koiwa K, Sato K, Ohtani Y, Takahashi T, Katoh K
  • Journal Title: Animal Science Journal Volume: 85(2) Issue: 3 Pages: 286-292
  • DOI: 10.1111/asj.12142
  • Peer Reviewed
• [Journal Article] Haplotype C of growth hormone (GH) gene in Japanese Black cattle: Structure of GH protein and a novel method for detection of the gene (2013)
  • Author(s): Bahrami A, Ardiyanti A, Tonosaki K, Suzuki K, Tanida N, Hirayama T, Roh SG, Nishio T, Katoh K.
  • Journal Title: Animal Science Journal Volume: 84 Issue: 12 Pages: 802-804
  • DOI: 10.1111/asj.12132
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms and protein structures in growth hormone, growth hormone receptor, ghrelin, insulin-like growth factor 1 and leptin in Mehraban sheep (2013)
  • Author(s): Bahrami A, Behzadi Sh, Miraei- Ashtiani S.R., Roh SG, Katoh K
  • Journal Title: Gene Volume: 527(1) Issue: 1 Pages: 397-404
  • DOI: 10.1016/j.gene.2013.05.066
  • Peer Reviewed
• [Journal Article] Effects of growth hormone gene polymorphism on lipogenic genes expression levels in diaphragm tissues of Japanese Black heifers (2012)
  • Author(s): Astrid Ardiyanti, Abe Tsuyoshi, Tameoka Nanae, Kobayashi Eiji, Shoji Noriaki, Ohtani Yoshihisa, Suzuki Keiichi, Roh Sang-gun and Kazuo Katoh
  • Journal Title: AJAS Volume: 25 Issue: 8 Pages: 1055-1062
  • DOI: 10.5713/ajas.2012.12029
  • Peer Reviewed
• [Journal Article] The regulation of chemerin and CMKLR1 genes expression by TNF-α, adiponectin and chemerin analog in bovine differentiated adipocytes (2012)
  • Author(s): Suzuki Y, Song SH, Ardiyanti A, Kato T, So KH, Katoh K, Roh SG
  • Journal Title: Asian-Australian Journal of Animal Science Volume: 25(9) Pages: 1316-1321
  • Peer Reviewed
• [Journal Article] Effects of bovine growth hormone (GH) gene polymorphism on lipogenic genes expression levels in diaphragm tissues of Japanese Black Heifer (2012)
  • Author(s): 11. Ardiyanti A, Abe T, Tameoka N, Kobayashi E, Shoji N, Suzuki K, Roh SG, Katoh K
  • Journal Title: Asian-Australian Journal of Animal Science Volume: 25(8) Pages: 1055-1062
  • Peer Reviewed
• [Journal Article] Changes in circulating adiponectin and metabolic hormones concentrations during periparturient and lactation periods in Holstein dairy cows (2012)
  • Author(s): Ohtani Y, Takahashi T, Sato K, Ardiyanti A, Song SH, Sato R, Onda K, Wada Y, Obara Y, Suzuki K, Hagino A, Roh SG, Katoh K
  • Journal Title: Animal Science Journal Volume: 83
  • Peer Reviewed
• [Journal Article] Chemerin analog regulates energy metabolism in sheep (2012)
  • Author(s): Suzuki, Y.ら
  • Journal Title: Animal Science Journal Volume: 83 Issue: 3 Pages: 263-267
  • DOI: 10.1111/j.1740-0929.2011.01002.x
  • Peer Reviewed
• [Presentation] 黒毛和種牛の内分泌変化と糖代謝に及ぼすGH 多型の影響 (2013)
  • Author(s): 小林諒子, Harajanti D.W., 盧尚建, 佐野 宏明, 加藤和雄
  • Organizer: 日本畜産学会第117回大会
  • Place of Presentation: 新潟大学五十嵐キャンパス(新潟)
• [Presentation] 成長ホルモンのCC 型黒毛和種牛の培養脂肪細胞の遺伝子発現について (2013)
  • Author(s): 加藤 大幾, 谷田奈々子, Astrid Ardiyanti, 鈴木 裕, 宋 相憲, 加藤和雄, 盧 尚建
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 安田女子大学キャンパス(広島)
• [Presentation] 黒毛和種牛の内分泌変化と糖代謝に及ぼすGH多型の影響 (2013)
  • Author(s): 小林諒子、Harajanti D.W.、盧尚建、佐野 宏明、加藤和雄
  • Organizer: 日本畜産学会第１７７大会
  • Place of Presentation: 新潟大学
• [Presentation] 黒毛和種牛の産肉性に及ぼすGH遺伝子プロモーター領域多型の影響 (2012)
  • Author(s): 杉田 春奈、加藤 大幾、Ardiyanti Astrid、横田 祥子、鈴木 啓一、笹子 奈々恵、阿部 剛、庄司 則章、小林 栄治、盧 尚建、加藤 和雄
  • Organizer: 日本畜産学会 第115回大会
  • Place of Presentation: 名古屋大学