Visualization of expression of gene increased the unsaturated fatty acids in meat
| Project/Area Number |
23658217
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Tohoku University |
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Principal Investigator |
KATOH Kazuo 東北大学, (連合)農学研究科(研究院), 教授 (60091831)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 食肉組成 / 不飽和脂肪酸 / SCD / 遺伝子導入 / 可視化 |
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| Research Abstract |
This study was to 1) investigate the gene expression of SCD in bovine adipocytes, and 2) generate delta-9-desaturease-GFP- knock in mice to visualize the activation of an enzyme generating a flavor and taste of the intramuscular fat.
1) Bovine GH (100 and 1000 ng/ml) decreased SCD mRNA expression in bovine differentiated adipocytes. 2) Using the homologous recombination method, embryo stem cell derived from the mouse embryo with GFP protein in an Exon1 of the internal delta-9 desaturase was established. The chimera mouse with delta-9-desaturease-GFP was identified and hetero delta-9-desaturease-GFP mouse generated.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Changes in circulating adiponectin and metabolic hormones concentrations during periparturient and lactation periods in Holstein dairy cows2012
Author(s)
Ohtani Y, Takahashi T, Sato K, Ardiyanti A, Song SH, Sato R, Onda K, Wada Y, Obara Y, Suzuki K, Hagino A, Roh SG, Katoh K
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Journal Title
Animal Science Journal
Volume: 83
Related Report
Peer Reviewed
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