| Project/Area Number |
23658226
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
MIYANO Takashi 神戸大学, 大学院・農学研究科, 教授 (80200195)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 卵母細胞 / 核小体 / 体外培養 / プロテアソーム |
|---|
| Research Abstract |
During the growth stage, oocyte nucleoli are intensively engaged in ribosomal RNA (rRNA) synthesis. As the oocytes approach the finalsize, synthetic activity of RNAs decreases, and coincidently nucleoli change the morphology and reduce the volume. Nucleoliof growing oocytes werecompacted and reduced the sizeby transcription inhibitors (actinomycin D or α-amanitin). On the other hand, the nucleolus volume of oocytes significantly increased by thetreatment with proteasome inhibitors, MG-132 or lactacystin. During oocyte maturation, the nucleolus was disassembled into the cytoplasm. When the oocytes were arrested at the germinal vesicle stage, and the nucleolus was aspirated from the nucleus and transferred to the cytoplasm, the nucleolus disappeared and later it was reassembled in the nucleus.
|
