Generation of rat iPS cells

• Project/Area Number: 23658227
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Toho University
• Principal Investigator: YANAI Kazuyuki 東邦大学, 理学部, 准教授 (30360704)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 発生工学 / iPS / モデル動物 / 再生医療 / iPS細胞 / ラット / 遺伝子導入ラット / 発現誘導

Research Abstract

We tried to generate iPS cells from our transgenic rat having drug-inducible reprogramming factors. However, there were some difficulties in mating. Then, as a first step to generate iPS cells and to analyze the reprogramming mechanisms, I focused on Oct4-binding sites in the nanog gene which is a marker gene of ES cells. I identified novel 9 novel Oct4-binding sequences in the rat nanog gene.

Research Products

• [Presentation] ヒトゲノムにおけるアンドロゲンレセプターの結合配列の系統的な同定とその解析 (2012)
  • Author(s): 村崎亜起子
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-11
• [Presentation] Genome-wide identification and characterization of Retinoid X Receptor homodimer binding sites by a genetic selection in yeast (2012)
  • Author(s): Hiroki Saito
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-11
• [Presentation] Genome-wide identification and characterization of Retinoic Acid Receptor/Retinoid X Receptor heterodimer binding sites by a genetic selection in yeast (2012)
  • Author(s): Takatsugu Kosugi
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-11
• [Presentation] Identification and Characterization of Human Genomic Binding Sites for Retinoic Acid Receptor/Retinoid X Receptor heterodimers (2012)
  • Author(s): Takatsugu Kosugi
  • Organizer: 生命医薬情報学連合大会
  • Place of Presentation: タワーホテル船堀(東京都)
  • Year and Date: 2012-10-25
• [Presentation] Identification and characterization of retinoic acid receptor/retinoid X receptor heterodimers binding sites in human genome (2012)
  • Author(s): Takatsugu Kosugi
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-10-25
• [Presentation] Identification and characterization of retinoic acid receptor/retinoid X receptor heterodimers binding sites in human genome.
  • Author(s): Takatsugu Kosugi
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 京王プラザホテル（東京都）
• [Presentation] Identification and Characterization of Human Genomic Binding Sites for Retinoic Acid Receptor/Retinoid X Receptor heterodimers
  • Author(s): Takatsugu Kosugi
  • Organizer: 生命医薬情報学連合大会
  • Place of Presentation: タワーホテル船堀（東京都）