Identification of factors that involved in migration of mesenchymal stem cells to lesions of neurodegenerative diseases

• Project/Area Number: 23658233
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: HORIUCHI Motohiro 北海道大学, 大学院・獣医学研究科, 教授 (30219216)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨髄由来間葉系幹細胞 / プリオン / 神経変性疾患 / 走化性 / ケモカイン

Research Abstract

In vitro migration assay was carried out to investigate chemoattractive factors for MSCs in the brain lesions of prion-infected mice. In addition, expression of chemokine receptor on MSCs that had been transplanted in the brain of prion-infected mice was analyzed. The combined in vitro and in vivo analyses suggest that CCR3, CCR5, CXCR3 and CXCR4 and their corresponding ligands are involved in the migration of hMSCs to the brain lesions caused by prion propagation.

Research Products

• [Journal Article] Therapeutic effect of peripheral administration of an anti-prion protein antibody on mice infected with prions (2013)
  • Author(s): Ohsawa, N., Song, C.-H., Suzuki, A., Furuoka, H., Hasebe, R., and Horiuchi,M
  • Journal Title: Microbiol. Immunol Volume: 57(4) Pages: 288-297
  • Peer Reviewed
• [Journal Article] Therapeutic effect of peripheral administration of an anti-prion protein antibody on mice infected with prions (2013)
  • Author(s): Ohsawa, N.
  • Journal Title: Microbiology and Immunology Volume: 57 Pages: n/a-n/a
  • DOI: 10.1111/j.1348-0421.12037
  • Peer Reviewed
• [Journal Article] Characterization of intracellular localization of PrPSc in prion-infected cells using monoclonal antibody that recognizes the region consisting of amino acids 119-127 of mouse (2012)
  • Author(s): Yamasaki, T., Suzuki, A., Shimizu, T., Watarai, M., Hasebe, R. and Horiuchi, M
  • Journal Title: PrP. J. Gen. Virol Volume: 93(Pt3) Pages: 668-680
  • Peer Reviewed
• [Journal Article] Reaction of complement factors varies with prion strains in vitro and in vivo (2012)
  • Author(s): Hasebe, R., Raymond, G. J., Horiuchi, M., and Caughey, B
  • Journal Title: Virology Volume: 423(2) Pages: 205-213
  • Peer Reviewed
• [Journal Article] Reaction of complement factors varies with prion strains in vitro and in vivo (2012)
  • Author(s): Hasebe, R.
  • Journal Title: Virology Volume: 423 Issue: 2 Pages: 205-213
  • DOI: 10.1016/j.virol.2011.11.017
  • Peer Reviewed
• [Journal Article] Characterization of intracellular localization of PrP^<Sc> in prioninfected cells using monoclonal antibody that recognizes the region consisting of amino acids 119-127 of mouse PrP (2012)
  • Author(s): Yamasaki T., Suzuki A., Shimizu T., Watarai M., Hasebe R., Horiuchi M.
  • Journal Title: Journal of General Virology Volume: vol.93 no.3 Issue: 3 Pages: 668-680
  • DOI: 10.1099/vir.0.037101-0
  • Peer Reviewed
• [Journal Article] Brucella abortus exploits a cellular prion protein on intestinal M cells as an invasive receptor. (2012)
  • Author(s): Nakato G, Hase K, Suzuki M, Kimura M, Ato M, Hanazato M, Tobiume M, Horiuchi M, Atarashi R, Nishida N, Watarai M, Imaoka K, Ohno H
  • Journal Title: Journal of Immunology Volume: 189(4) Issue: 4 Pages: 1540-4
  • DOI: 10.4049/jimmunol.1103332
  • Peer Reviewed
• [Journal Article] Alternative BSE Risk Assessment Methodology of Imported Beef and Beef Offal to Japan (2012)
  • Author(s): Yoshikawa, Y.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Pages: 959-968
  • Peer Reviewed
• [Journal Article] Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions (2011)
  • Author(s): Song C.-H., Honmou, O., Furuoka, H. and Horiuchi, M
  • Journal Title: J. Virol Volume: 85(21) Pages: 11069-11078
  • NAID: 120004027230
  • Peer Reviewed
• [Journal Article] Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prion (2011)
  • Author(s): Song, C.H., et al
  • Journal Title: J.Virol. Volume: 85 Issue: 21 Pages: 11069-11078
  • DOI: 10.1128/jvi.05318-11
  • Peer Reviewed
• [Presentation] Construction of mAb132-EGFP fusion proteins as a PrPSc-specific probe (2012)
  • Author(s): Suzuki, A., Yamasaki, T., Hasebe, R., and Horiuchi, M
  • Organizer: Asia Pacific Prion Symposium 2012
  • Place of Presentation: Yokoyama,Japan
• [Presentation] Analysis of PrPSc accumulation and glial cell activationin brains of prion-infected mice at the early stage of infection (2012)
  • Author(s): Horiuchi, M, Yamasaki, T., Hasebe, R. and Takahashi, Y
  • Organizer: Prion2012
  • Place of Presentation: Amsterdam, The Netherland)UV University Amsterdam
• [Presentation] Analysis of PrPSc accumulation and glial cell activation in brains of prion-infected mice at the early stage of infection (2012)
  • Author(s): Horiuchi, M.
  • Organizer: Prion2012
  • Place of Presentation: VU University, Amsterdam, The Netherland
• [Presentation] A decreased expression of pre-synaptic markers in neurons in differentiated neurospheres infected with BSE-derived prion strain (2012)
  • Author(s): Sassa, Y.
  • Organizer: Prion2012
  • Place of Presentation: VU University, Amsterdam, The Netherland
• [Presentation] Analysis of intracellular dynamics of inoculated PrP-res and newly generated PrPSc in Neuro2a cells (2012)
  • Author(s): Yamasaki, T.
  • Organizer: Asia Pacific Prion Symposium 2012
  • Place of Presentation: Pacifico Yokohama, Yokoyama, Japan
• [Presentation] Immuno- and cell-therapy as possible treatment for prion diseases (2012)
  • Author(s): Horiuchi, M.
  • Organizer: BBSRC Japan Partnering Awards
  • Place of Presentation: The Roslin Institute & Royal (Dick) School of Veterinary Sciences, Edinburgh, UK
  • Invited
• [Presentation] Immunohistochemical analysis of PrPSc accumulation and activation of astrosytes and microglia in early stage of prion infection (2011)
  • Author(s): Takahashi, Y., Song, C.-H., Yamasaki, T., Hasebe, R., and Horiuchi, M
  • Organizer: APPS2011
  • Place of Presentation: Karuizawa,Japan Hotel Marroad Karuizawa
• [Presentation] Detection of newly generataed PrPSc in Neuro2a cells inoculated with fluorescent-dye labeled purified PrPSc (2011)
  • Author(s): Yamasaki, T., Garon, G. B., and Horiuchi, M
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo, Japan Sapporo Convention Center
• [Presentation] 異常型プリオンタンパク質の細胞内増殖 (2011)
  • Author(s): 堀内 基広、山崎 剛士
  • Organizer: 第30回日本認知症学会(招待講演)
  • Place of Presentation: タワーホール船堀（東京）
• [Presentation] Characterization of prion infection in differentiated mouse neurospheres. (2011)
  • Author(s): Sassa, Y., Yamasaki, T., Hasebe, R., and Horiuchi, M.
  • Organizer: Prion2011
  • Place of Presentation: Fairmont Queen Elizabeth (Canada)
• [Presentation] Involvement of the endosomal Cl-/H+ antiporter Clc-5 in the formation of PrP-res in prion-infected Neuro2a mouse neuroblastoma cells. (2011)
  • Author(s): Nakamitsu, S., Sassa, Y., Yamasaki, T., Hasebe, R., and Horiuchi, M.
  • Organizer: APPS2011
  • Place of Presentation: Hotel Marroad Karuizawa (Japan)
• [Presentation] Characterization of prion infection in differentiated mouse neurospheres. (2011)
  • Author(s): Yamasaki, T., Garon, G. B., and Horiuchi, M.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo Convention Center (Japan)
• [Book] プリオンの感染と細胞内動態 (2013)
  • Author(s): 山崎剛士、堀内基広
  • Publisher: Dementia Japan
• [Book] プリオン病最新医学 (2011)
  • Author(s): 堀内基広
• [Book] 最新医学 (2011)
  • Author(s): プリオン病
  • Total Pages: 8
  • Publisher: 最新医学社
• [Book] 獣医微生物学（第3版） (2011)
  • Author(s): プリオンとプリオン病
  • Total Pages: 6
  • Publisher: 文永堂出版