Development of the functional mutagenesis system on Plasmodium prarasite

• Project/Area Number: 23658235
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUMOTO Shinya 帯広畜産大学, 原虫病研究センター, 准教授 (50376422)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: マラリア / ベクター / 変異体 / ハマダラカ

Research Abstract

Genetic mutant is a powerful tool for the infectious disease research. Thus, the development of mutant screening system in the parasitic disease in veterinary field is desired. In this study, we tried to develop the transposon based random mutant screening system using murine malaria parasite as a model pathogen. As a result, we successfully established the gain-of function mutant parasite library system. Furthermore, with this system, we successfully could identify the genomic locus that highly concerns to vector transmission.

Research Products

• [Journal Article] Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity (2013)
  • Author(s): Bando, H. Okado, K. Guelbeogo, W. M. Badolo, A. Aonuma, H. Nelson, B. Fukumoto, S. Xuan, X. Sagnon, N. Kanuka, H.
  • Journal Title: Sci Rep Volume: 3 Issue: 1 Pages: 1641-1641
  • DOI: 10.1038/srep01641
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila. (2013)
  • Author(s): Bryce Nelson, et al.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 430 Issue: 3 Pages: 1120-1125
  • DOI: 10.1016/j.bbrc.2012.12.027
  • Peer Reviewed
• [Journal Article] Expression profiles of peroxiredoxins in liver stage of the rodent malari a parasite Plasmodium berghei (2013)
  • Author(s): Usui M, Masuda-Suganuma H, Fukumoto S, Angeles JM, Inoue N, Kawazu S.
  • Journal Title: Parasitology International Volume: Volume 62 Issue: 3 Pages: 337-340
  • DOI: 10.1016/j.parint.2012.11.007
  • Peer Reviewed
• [Journal Article] Multivariate analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium yoelii (2012)
  • Author(s): Saiki E, Nagao K, Aonuma ll, Fukumoto S. Xuan X, Bannai M, Kanuka H
  • Journal Title: Malaria Journal Volume: 12 Issue: 1 Pages: 19-19
  • DOI: 10.1186/1475-2875-12-19
  • Peer Reviewed
• [Journal Article] Mitochondrial peroxidase TPx-2 is not essential in the blood and insect stages of Plasmodium berghei. (2012)
  • Author(s): Masuda-Suganuma, H., Usui, M., Fukumoto, S., Inoue, N.Kawazu, S.
  • Journal Title: Parasit Vectors Volume: 5 Issue: 1 Pages: 252-252
  • DOI: 10.1186/1756-3305-5-252
  • Peer Reviewed
• [Journal Article] Development of an allele-specific, loop-mediated isothermal amplification method(AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s.l. (2012)
  • Author(s): Badolo A, 0kado K, Guelbeogo 〓, Aonuma H, Bando H, Fukumoto S, Sagnoll N, kanuka H.
  • Journal Title: Malaria Journal Volume: 11 Issue: 1 Pages: 227-227
  • DOI: 10.1186/1475-2875-11-227
  • Peer Reviewed
• [Journal Article] In vitro and in vivo antimalarial activities of T-2307, a novel arylamidine. (2012)
  • Author(s): Kimura, A., Nishikawa, H., Nomura, N., Mitsuyama, J., Fukumoto, S., Inoue, N.Kawazu, S.
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Issue: 4 Pages: 2191-3
  • DOI: 10.1128/aac.05856-11
  • Peer Reviewed
• [Journal Article] Calcium signal regulates temperature-dependent transformation of sporozoites in malaria parasite development (2011)
  • Author(s): Doi, Y. Shinzawa, N. Fukumoto, S. Okano, H. Kanuka, H.
  • Journal Title: Exp Parasitol Volume: 128 Issue: 2 Pages: 176-80
  • DOI: 10.1016/j.exppara.2011.02.011
  • Peer Reviewed
• [Journal Article] Improvement of the observational method for Plasmodium berghei oocysts in the midgut of mosquitoes (2011)
  • Author(s): Usui, M. Fukumoto, S. Inoue, N. Kawazu, S.
  • Journal Title: Parasit Vectors Volume: 4 Issue: 1 Pages: 118-118
  • DOI: 10.1186/1756-3305-4-118
  • Peer Reviewed
• [Presentation] Amino acid-related host nutrition dynamics during malaria infection (2013)
  • Author(s): Saiki E, Nagao K, Fukumoto S, Bannai M, Kanuka H
  • Organizer: Keystone Symposia on Molecular and Cellular Biology : Malaria
  • Place of Presentation: JW Marriott New Orleans, New Orleans, Louisiana USA
• [Presentation] Development of LAMP-based method to detect 1014F kdr and 119S ace-1R mutations in field collected Anopheles gambiae s.l. (2013)
  • Author(s): A. Badolo A., Fukumoto S., Okado K., Aonuma H., Guelbeogo W.M., Bando H., Yoshimura A., Sagnon N’F and Kanuka H.
  • Organizer: EMBO conference on Molecular and Population Biology of Mosquitoes and Other Disease Vectors: From Basic Vector Biology to Disease Control
  • Place of Presentation: Orthodox Academy Of Crete, Crete, Greece.
• [Presentation] 西アフリカに生息するマラリア媒介蚊の腸内細菌種内多様性に関する研究 (2013)
  • Author(s): 伴戸寛徳、岡戸清、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 第156回日本獣医学会
  • Place of Presentation: 岐阜大学（ 岐阜県岐阜市 ）
• [Presentation] Development of LAMP-based method to detect 1014F kdr and 119S ace-1R mutations in field collected Anopheles gambiae s.l. (2013)
  • Author(s): A. Badolo A., Fukumoto S., Okado K., Aonuma H., Guelbeogo W.M., Bando H., Yoshimura A., Sagnon N’F and Kanuka H.
  • Organizer: 6th MIM Pan-African Malaria Conference.
  • Place of Presentation: The International Convention Centre, Durban, South Africa
• [Presentation] マラリアをモデルとした重症化と宿主血中アミノ酸ダイナミクスの相関解析 (2013)
  • Author(s): 齊木 選射，青沼 宏佳，長尾 健児，福本 晋也，坂内 慎，嘉糠 洋陸
  • Organizer: 第36 回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド（ 兵庫県神戸市 ）
• [Presentation] マラリア原虫感染時の宿主血中アミノ酸インフォマティクス (2013)
  • Author(s): 齋木選射、長尾健児、福本晋也、坂内慎、嘉糠洋陸
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京
• [Presentation] 非共生細菌の表現型揺らぎが規定するベクター・寄生虫間相互作用 (2013)
  • Author(s): 嘉糠洋陸、伴戸寛徳、岡戸清、Guelbego Wamdaogo､Badolo Athanase､青沼宏佳、福本晋也、NFale Sagnon
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京
• [Presentation] Impact of intra-specific diversity of mosquito midgut bacteria on Plasmodium development (2011)
  • Author(s): Hironori Bando, Kiyoshi Okado, Moussa Guelbeogo, Athanase Badolo, Hiroka Aonuma, Shinya Fukumoto, N'Fale Sagnon, and Hirotaka Kanuka
  • Organizer: 第80回米国熱帯医学会
  • Place of Presentation: フィラデルフィア・ペンシルバニア
• [Presentation] Development of allele-specific loop-mediated isothermal amplification method (AS-LAMP) for detection of the L1104Fkdr- mutation in Anopheles gambiae s. 1 (2011)
  • Author(s): Badolo A. Okado K., Aonuma H., Guelbeogo W.M., Bando H., Yoshimura A. Fukumoto S., Sagnon N'F and Kanuka H
  • Organizer: Gordon Research conference
  • Place of Presentation: Pisa, ItalyJuly