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Fundamental studies for development of a novel anti-tumor drug based on perchloric acid-soluble protein

Research Project

Project/Area Number 23658244
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

KANOUCHI HIROAKI  鹿児島大学, 獣医学部, 准教授 (10351884)

Co-Investigator(Kenkyū-buntansha) FUJIKI Makoto  鹿児島大学, 共同獣医学部, 准教授 (60305167)
刀袮 重信  川崎医科大学, 医学部, 准教授 (70211399)
Co-Investigator(Renkei-kenkyūsha) TONE Shigenobu  川崎医科大学, 医学部, 准教授 (70211399)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsガン細胞 / 細胞増殖 / タンパク質高次構造変化 / 抗がん剤 / ケミカルライブラリー / レポーターアッセイ / タンパク質高次構造
Research Abstract

Perchloric acid-soluble protein (PSP) has an anti-proliferating activity. Its activity seems to be suppressed in cells, because the PSP exists in proliferating cells. Crystal structural analysis of PSP shows that PSP exist as a homo-trimer. Our preliminary experiment, however, suggested PSP existed not only timer but also monomer or dimer. To elucidate that changing higher-order protein structure of PSP is related to cell proliferation, we evaluated the state of higher-order protein structure of PSP during liver regeneration after partial hepatectomy in rats. As a result, PSP existed as trimer in normal liver, but the ratio of trimer of PSP decreased after hepatectomy and the ration of monomer or dimer of PSP increased until liver weight returned to the intact weight. It is suspected monomer or dimer PSP not to suppress cell proliferation. Additionally, we identified the DNA position for expression and regulation of PSP mRNA. We identified two molecules to induce PSP expression.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Remarks (2 results)

  • [Journal Article] Plasma Homocysteine Concentrations in Novel Microminipigs2014

    • Author(s)
      Toshiaki Kakimoto, Akira Otsuka, Hiroaki Kawaguchi, Kenji Ogata, Akihide Tanimoto, Hiroaki Kanouchi
    • Journal Title

      In Vivo

      Volume: 28

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low pyridoxine concentrations enhance lipopolysaccharide-stimulated gene expression of cyclooxygenase-2 and inducible nitric oxide synthase in RAW264.7 cells2013

    • Author(s)
      Kanouchi H
    • Journal Title

      J. Nutr. Sci. Vitaminol

      Volume: 59(6) Pages: 548-51

    • NAID

      130003393752

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/24477252

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low Pyridoxine Concentrations Enhance Lipopolysaccharide-Stimulated Gene Expression of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in RAW264.7 Cells2013

    • Author(s)
      Hiroaki Kanouchi
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 59 Issue: 6 Pages: 548-551

    • DOI

      10.3177/jnsv.59.548

    • NAID

      130003393752

    • ISSN
      0301-4800, 1881-7742
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Homocysteine induced SH-SY5Y apoptosis through activation of NADPH oxidase in U251-MG cells2012

    • Author(s)
      Fujiki Y, Hirashima Y, Seshimo S, Okamoto T, Sugimoto Y, Matsumoto M, Oka T, Kanouchi H
    • Journal Title

      Neurosci. Res

      Volume: 72(1) Issue: 1 Pages: 9-15

    • DOI

      10.1016/j.neures.2011.09.010

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Decomposition of insoluble and hard-to-degrade animal proteins by enzyme E77 and its potential applications.2012

    • Author(s)
      Zhao H, Mitsuiki S, Takasugi M, Sakai M, Goto M, Kanouchi H, Oka T.
    • Journal Title

      Appl Biochem Biotechnol.

      Volume: 166 Issue: 7 Pages: 1758-1768

    • DOI

      10.1007/s12010-012-9581-3

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] High Dose of Pyridoxine Induces IGFBP-1 mRNA Expression in MCF-7 Cells and Its Induction is Inhibited by the p53-specific Inhibitor Pifithrin-α2011

    • Author(s)
      Nakari M, Kanouchi H, Oka T
    • Journal Title

      J. Nutr. Sci.Vitaminol

      Volume: 57(4) Pages: 280-284

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22041910

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] High Dose of Pyridoxine Induces IGFBP-3 mRNA Expression in MCF-7 Cells and Its Induction Is Inhibited by the p53-Specific Inhibitor Pifithrin-.ALPHA.2011

    • Author(s)
      Nakari Miwa, Kanouchi Hiroaki, Oka Tatsuzo.
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 57 Issue: 4 Pages: 280-284

    • DOI

      10.3177/jnsv.57.280

    • NAID

      130001372999

    • ISSN
      0301-4800, 1881-7742
    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Remarks]

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-Mol/bunshibyoutai01/Welcome.html

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-Mol/bunshibyoutai01/Welcome.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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