For decrudescence of modern diseases: Establishment of a microsporidia vector carrying functional protein of modern diseases

Research Project

Project/Area Number	23658254
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Applied veterinary science
Research Institution	Kitasato University
Principal Investigator	IKADAI Hiromi 北里大学, 獣医学部, 准教授 (80327460)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	寄生虫 / 原虫 / 微胞子虫 / 遺伝子組換え / 遺伝子導入
Research Abstract	As for Encephalitozoon cuniculi, the genetic manipulationmethod is not established. In this study, we tried to establishment of the genetic manipulation method for E. cuniculiand obtained the following results. (I) Establishment of purification of sporesfrom in vitroculturedE. cuniculi-infected cells. (II)neoR gene became a candidate as a drug selective marker of E. cuniculi. (III)The condition of the genetic manipulation method by the electroporation was decided