| Project/Area Number |
23658255
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied veterinary science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KITAMURA Hiroshi 名古屋私立大学, 医学系研究科, 准教授 (80312403)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | がん幹細胞 |
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| Research Abstract |
To develop a novel method for therapy targetting cancer stem cell (CSC) in dogs, the monoclonal antibodies (mAbs) against canine ortholog of CSC markers in human were produced. Some mAbs against canine CD (cCD) 133 or cCD29 proteins that was expressed by Escherichia coli expression system were respectively obtained. Western blot analyses revealed that all of anti-cCD133 mAbs could not detect a native CD133 protein in a membrane fraction of born marrow and did not recognized the CD133 proteins expressed in HEK293 cells. On the other hand, in immunohistochemical study using an anti-cCD29 mAb, mesenchymal cells in basivertebral cartilage and blood vessel were positively stained in a dog embryo. Further study is needed to produce anti-cCD133mAbs that can recognize a native cCD133 and to verify the usefulness of anti-cCD29mAbs in sorting of mesenchymal stem cell.
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