Analysis of novel neoplastic mechanisms of canine mast cell tumors by using a retroviral expression library method.
| Project/Area Number |
23658260
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical veterinary science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OHMORI Keitaro 東京農工大学, 大学院・農学研究院, 講師 (20466915)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イヌ / 腫瘍 / 遺伝子 / 肥満細胞 / レトロウイルス |
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| Research Abstract |
Mutations in c-kit gene have been reported to be important as a neoplastic mechanism in canine mast cell tumors (MCTs). In the present study, we investigated the incidence of mutations in c-kit gene in canine MCTs in Japan, and explored novel neoplastic mechanisms by using a retroviral expression library method. We found that mutations in c-kit gene were not detected in more than half of dogs with MCTs. We, next, created retrovirus and tried to establish a retrovirus-mediated gene transfer system to canine bone marrow derived mast cells. However, since transduction efficiency of retrovirus generated in this study was low, we could not identify c-kit gene-independent neoplastic mechanisms of canine MCTs.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Daily intake of Jeju groundwater improves the skin condition of the model mouse for human atopic dermatitis.2013
Author(s)
Tanaka A, Jung K, Matsuda A, Jang H, Kajiwara N, Amagai Y, Oida K, Ahn G, Ohmori K, Kang KG, Matsuda H
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Journal Title
Journal of Dermatology
Volume: 40
Issue: 3
Pages: 193-200
DOI
Related Report
Peer Reviewed
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[Journal Article] Supplementation of the fermented soy product ImmuBalanceTM effectively reduces itching behavior of atopic NC/Tnd mice2012
Author(s)
Matsuda A, Tanaka A, Pan W, Okamoto N, Oida K, Kingyo N, Amagai Y, Xia Y, Jang H, Nishikawa S, Kajiwara N, Ahn G, Ohmori K, Matsuda H
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Journal Title
J. Dermatol. Sci
Volume: 67(2)
Issue: 2
Pages: 130-139
DOI
Related Report
Peer Reviewed
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[Journal Article] Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells2011
Author(s)
Matsuda A, Tanaka A, Amagai Y, Ohmori K, Nishikawa S, Xia Y, Karasawa K, Okamoto N, Oida K, Jang H, Matsuda H.
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Journal Title
Vet Immunol Immunopathol
Volume: 144
Issue: 3-4
Pages: 321-328
DOI
Related Report
Peer Reviewed
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[Journal Article] Peroxisome proliferator-activated receptorγ-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/Tnd mice2011
Author(s)
Jung K, Tanaka A, Fujita H, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H.
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Journal Title
J Allergy Clin Immunol
Volume: 127
Issue: 2
Pages: 420-429
DOI
Related Report
Peer Reviewed
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[Journal Article] Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells2011
Author(s)
Matsuda A, Tanaka A, Amagai Y, Ohmori K, Nishikawa S, Xia Y, Karasawa K, Okamoto K, Oida K, Jang H, Matsuda H.
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Journal Title
Vet. Immunol. Immunopathol.
Volume: 144
Pages: 321-328
Related Report
Peer Reviewed
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