| Project/Area Number |
23658260
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical veterinary science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OHMORI Keitaro 東京農工大学, 大学院・農学研究院, 講師 (20466915)
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イヌ / 腫瘍 / 遺伝子 / 肥満細胞 / レトロウイルス |
|---|
| Research Abstract |
Mutations in c-kit gene have been reported to be important as a neoplastic mechanism in canine mast cell tumors (MCTs). In the present study, we investigated the incidence of mutations in c-kit gene in canine MCTs in Japan, and explored novel neoplastic mechanisms by using a retroviral expression library method. We found that mutations in c-kit gene were not detected in more than half of dogs with MCTs. We, next, created retrovirus and tried to establish a retrovirus-mediated gene transfer system to canine bone marrow derived mast cells. However, since transduction efficiency of retrovirus generated in this study was low, we could not identify c-kit gene-independent neoplastic mechanisms of canine MCTs.
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