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Isolation of monochlorophenol-dechlorinating microorganisms using bio-electrochemical enrichment system with plug flow under anaerobic conditions

Research Project

Project/Area Number 23658272
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Boundary agriculture
Research InstitutionNagoya University

Principal Investigator

KATAYAMA Arata  名古屋大学, エコトピア科学研究所, 教授 (60185808)

Co-Investigator(Kenkyū-buntansha) SUZUKI Daisuke  名古屋大学エコトピア科学研究所, 嫌気微生物学, 助教 (10591076)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords環境修復 / 電気培養集積系 / 固体電子メディエータ / モノクロロフェノール / ポリクロロフェノール / 脱塩素 / 炭素源 / 分解促進 / 微生物集積 / 電気培養 / 還元的脱塩素反応 / 微生物群集構造 / 回分培養 / 脱塩素微生物 / 発酵性微生物 / クロロフェノール / 電子メディエータ
Research Abstract

Anaerobic dechlorination is the important reaction in the bioremediation of chlorinated aromatic compounds for decreasing persistency and toxicity of the compounds. There are many reports on the isolation of anaerobic dechlorinator for the polychlorinated aromatic compounds. However, monochlorophenol-dechlorinating anaerobic microorganisms have not been isolated except for few exception. In this study, we have conducted to isolate the monochlorophenol-dechlorinating anaerobic microorganisms. The bio-electrochemical enrichment system, or the enrichment column system with anaerobic plug flow did not enhance the dechlorination of mono-chlorophenol but poly-chlorophenols. Various trials under different conditions suggested that mono-chlorophenol dechlorinating culture did not require other carbon sources such as lactate and formate. Thus, we provided the anaerobic enrichment systems using mono-chlorophenol or phenol as sole source of carbon and energy. The enrichment with phenol resulted in the isolation of anaerobe growing on phenol degradation under sulfate-reducing conditions. The enrichment with 3-chlorophenol was successfully obtained and 3-chlorophenol-dechlorinating anaerobe was identified. These findings expands our knowledge on the mono -chlorophenol dechlorinators and their characteristics.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (20 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Use of a glass bead-containing liquid medium for efficient production of a soil-free culture with polychlorinated biphenyl-dechlorination activity2013

    • Author(s)
      Daisuke Suzuki, Daisuke Baba, Velayudhan Satheeja Santhi, Robinson David Jebakumar Solomon, and Arata Katayama
    • Journal Title

      World Journal of Microbiology and Biotechnology

      Volume: 29 Issue: 8 Pages: 1461-1471

    • DOI

      10.1007/s11274-013-1310-8

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polyphasic characterization of two microbial consortia with wide dechlorination spectra for chlorophenols2012

    • Author(s)
      Chunfang Zhang, Daisuke Suzuki, Zhiling Li, Lizhen Ye, Arata Katayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 114(5) Issue: 5 Pages: 512-517

    • DOI

      10.1016/j.jbiosc.2012.05.025

    • NAID

      110009553583

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Humin as an electron mediator for microbial reductive dehalogenation2012

    • Author(s)
      Chunfang Zhang, Arata Katayama
    • Journal Title

      Environmental Science and Technology

      Volume: 46 Issue: 12 Pages: 6575-6583

    • DOI

      10.1021/es3002025

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simulation of the Reductive Dechlorination Processes in a Lab-Scale Anaerobic Biobarrier with the Enriched TCP Dechlorination Consortium2012

    • Author(s)
      Zhiling Li, Yasushi Inoue, Takuya Mizoguchi, Yohei Simizu, Naoko Y oshida andArata Katayama
    • Journal Title

      Transactions of Tianjin University

      Volume: 18 Issue: 6 Pages: 441-449

    • DOI

      10.1007/s12209-012-1768-8

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 3-クロロフェノール脱塩素活性に対する硫黄還元細菌,硫酸還元細菌およびメタン生成古細菌の影響2012

    • Author(s)
      鈴木大典,片山新太
    • Organizer
      日本農芸化学会 2012 年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2012 Final Research Report
  • [Presentation] Polyphasic characterization of two anaerobic microbial consortia dechlorinating wide spectra of phenols with lower levels of chlorination2012

    • Author(s)
      Chunfang ZHANG , Daisuke SUZUKI, Zhiling LI, Lizhen YE, Arata KATAYAMA
    • Organizer
      WET2012 Water and Environment Technology Conference
    • Place of Presentation
      The University of Tokyo, Tokyo, Japan
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Characteristics of a 4-chlorophenol degrading, sulfate-reducing bacterium isolated from a river sediment contaminated with chlorinated solvents2012

    • Author(s)
      Daisuke SUZUKI, Arata KATAYAMA
    • Organizer
      The power of the small-the 14thInternational Symposium on Microbial Ecology -ISME-14
    • Place of Presentation
      The Bella Center , Copenhagen Denmark
    • Related Report
      2012 Final Research Report
  • [Presentation] Long-term Anaerobic Mineralization of PCP in a Continuous-Flow System Using Only Lactate as an External Nutrient2012

    • Author(s)
      Zhiling LI, Yasushi INOUE, Daisuke SUZUKI, Lizhen YE and Arata KATAYAMA
    • Organizer
      the 4thInternational Conference on Soil Pollution and Remediation (SOILREM 2012)
    • Place of Presentation
      Yantai Oriental Haitian Hotel,No. 12 Haiyun Rd. Laishan, China
    • Related Report
      2012 Final Research Report
  • [Presentation] Microbial cofinment technology of groundwater contaminated with halogenated organic pollutants2012

    • Author(s)
      Arata Katayama, Zhiling Li, Chunfang Zhang, Dongdong Zhang, Suyin Yang, and Daisuke Suzuki
    • Organizer
      First International Symposium on Advanced Water Science and Technology (ISAWST-1)
    • Place of Presentation
      Noyori Conference Hall, Nagoya University, Nagoya, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Microbial reductive dechlorination of pentachlorophenol mediated by humin2012

    • Author(s)
      Chunfang Zhang, Zhiling Li, Daisuke Suzuki, and Arata Katayama
    • Organizer
      First International Symposium on Advanced Water Science and Technology (ISAWST-1)
    • Place of Presentation
      Noyori Conference Hall, Nagoya University, Nagoya, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Influence of electron donors and electron acceptors on the 3-chlorophenol dechlorination activity of anaerobic enrichment culture2012

    • Author(s)
      Daisuke Suzuki, Zhiling Li, Chunfang Zhang and Arata Katayama
    • Organizer
      First International Symposium on Advanced Water Science and Technology (ISAWST-1)
    • Place of Presentation
      Noyori Conference Hall, Nagoya University, Nagoya, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] 3-クロロフェノール脱塩素嫌気性集積培養物における脱塩素菌の推定2012

    • Author(s)
      鈴木大典、片山新太
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学、豊橋
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Characteristics of a 4-chlorophenol degrading, sulfate-reducing bacterium isolated from a river sediment contaminated with chlorinated solvents2012

    • Author(s)
      Daisuke SUZUKI
    • Organizer
      the 14th International Symposium on Microbial Ecology –ISME-14
    • Place of Presentation
      The Bella Center , Copenhagen Denmark
    • Related Report
      2012 Annual Research Report
  • [Presentation] Long–term Anaerobic Mineralization of PCP in a Continuous–Flow System Using Only Lactate as an External Nutrient2012

    • Author(s)
      Zhiling LI
    • Organizer
      the 4th International Conference on Soil Pollution and Remediation (SOILREM 2012)
    • Place of Presentation
      Yantai Oriental Haitian Hotel, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] Microbial cofinment technology of groundwater contaminated with halogenated organic pollutants2012

    • Author(s)
      Arata Katayama
    • Organizer
      First International Symposium on Advanced Water Science and Technology (ISAWST-1)
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Microbial reductive dechlorination of pentachlorophenol mediated by humin2012

    • Author(s)
      Chunfang ZHANG
    • Organizer
      First International Symposium on Advanced Water Science and Technology (ISAWST-1)
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Influence of electron donors and electron acceptors on the 3-chlorophenol dechlorination activity of anaerobic enrichment culture2012

    • Author(s)
      Daisuke SUZUKI
    • Organizer
      First International Symposium on Advanced Water Science and Technology (ISAWST-1)
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 3-クロロフェノール脱塩素活性に対する硫黄還元細菌,硫酸還元細菌およびメタン生成古細菌の影響2012

    • Author(s)
      鈴木大典,片山新太
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] nfluencing factors on monochlorophenol-dechlorinating activities of anaerobic microbial communities enriched from a contaminated river sediment2011

    • Author(s)
      Daisuke SUZUKI, Arata KATAYAMA
    • Organizer
      The 4th Congress of European Microbiologists
    • Place of Presentation
      Genova, Switzerland
    • Related Report
      2012 Final Research Report
  • [Presentation] Composition of microbial populations of polychlorinated biphenyl-dechlorinating soil-free culture2011

    • Author(s)
      Daisuke SUZUKI, Daisuke BABA, Velayudhan Satheeja SANTHI, Robinson David Jebakumar SOLOMON, Arata KATAYAMA
    • Organizer
      International Symposium on EcoTopia Science 2011
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] 3-モノクロロフェノール脱塩素嫌気性集積培養物の細菌群集構成とクロロフェノール脱塩素活性2011

    • Author(s)
      鈴木大典,片山新太
    • Organizer
      第27回日本微生物生態学会
    • Place of Presentation
      京都大学
    • Related Report
      2012 Final Research Report
  • [Presentation] Influencing factors on monochlorophenol-dechlorinating activities of anaerobic microbial communities enriched from a contaminated river sediment2011

    • Author(s)
      Daisuke SUZUKI, Arata KATAYAMA
    • Organizer
      The 4th Congress of European Microbiologists
    • Place of Presentation
      Genova, Switzerland
    • Related Report
      2011 Research-status Report
  • [Presentation] Composition of microbial populations of polychlorinated biphenyl-dechlorinating soil-free culture2011

    • Author(s)
      Daisuke SUZUKI, Daisuke BABA, Velayudhan Satheeja SANTHI, Robinson David Jebakumar SOLOMON, Arata KATAYAMA
    • Organizer
      International Symposium on EcoTopia Science 2011
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] 3-モノクロロフェノール脱塩素嫌気性集積培養物の細菌群集構成とクロロフェノール脱塩素活性2011

    • Author(s)
      鈴木大典,片山新太
    • Organizer
      第27回日本微生物生態学会
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Book] 微生物の辞典2013

    • Author(s)
      鈴木大典
    • Publisher
      朝倉書店
    • Related Report
      2012 Annual Research Report
  • [Book] 93.人に役立つ土壌微生物 「微生物の事典」

    • Author(s)
      鈴木大典、片山新太
    • Publisher
      朝倉書店
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.er.esi.nagoya-u.ac.jp/rescwe/tairyou/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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