| Project/Area Number |
23658281
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
HARATA Masahiko 東北大学, (連合)農学研究科(研究院), 准教授 (70218642)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エピジェネティック / クロマチン / 転写 / 出芽酵母 / 遺伝子発現 / 細胞核 / エピジェネティクス / アクチン関連タンパク質 / クロマチンリモデリング / エピジェネティック制御 |
|---|
| Research Abstract |
We deleted the budding yeast ARP6 gene and analyzed the expression of genes responsible for ethanol fermentation and spacial localization of genes in the nucleus. We also analyzed the spacial localization of a chromatin regions to which Arp6 or a histone variant H2A.Z was artificially bound. Using chromatin immunoprecipitation assay, it was shown that Arp6-bound chromatin bond to nuclear pore complex and that H2A.Z-bound chromatin bonds also to Mps3, a nuclear membrane protein. These observations suggests that these nuclear membrane proteins have roles in the spatial arrangement of genes, including ones for ethanol fermentation.
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