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Analysis of the molecular basis for mRNA dynamics in the nucleus using next generation sequencer and the function for the factor(s)of quality control.

Research Project

Project/Area Number 23658289
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionKyoto University

Principal Investigator

MASUDA Seiji  京都大学, 大学院・生命科学研究科, 准教授 (20260614)

Co-Investigator(Renkei-kenkyūsha) OKUMURA Katsuzumi  三重大学, 大学院・生物資源学研究科, 教授 (30177183)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsmRNA / 次世代シークエンサ / 品質管理因子 / RNA / 品質管理
Research Abstract

The mRNA quality control in the nucleus remains largely unknown. Here, to analyze the molecular function of mRNA quality control factors in the nucleus, whole transcriptome analysis of the mRNA dynamics in the nucleus was exploited. And the importance of the mRNA quality control was also investigated using the conventional biochemical and molecular biology methods. The whole transcriptome was analyzed from the cells treated with siRNA of RRP45, a core component of exosome, or MTR4, a subcomponent of exosome. Whole reads were mapped on the human genome and the changes of the number of reads mapped on the specific region were compared between siRNA treated- and control cells. The genes whose expressions were markedly increased wereselected by the treatment of siRNA of RRP45 or MTR4. Next, the identification and localization of human TRAMP (PAPD5-ZCCHC7-MTR4) complex were analyzed using conventional methods like immunoprecipitation and immunostaining. Exosome and MTR4 were localized in the nucleolus. PAPD5 and ZCCHC7 were localized in the nucleus. The candidate molecules of human TRAMP complex were associated in the nuclear extract.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (17 results) Book (3 results) Remarks (1 results)

  • [Journal Article] mRNA 核外輸送の効率化による動物細胞タンパク質生産2012

    • Author(s)
      松村 嘉員、増田 誠司
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 70 Pages: 373-375

    • NAID

      10031003447

    • URL

      http://www.jba.or.jp/pc/bi/

    • Related Report
      2012 Final Research Report
  • [Journal Article] Establishment of the monitoring system which detects the inhibition of mRNA processing.2012

    • Author(s)
      Fujita, K-I., Okamura, M., Nishimoto, S., Kurihara, T., Momma, K., Miyamae, Y., Kambe, T., Nagao, M., Narita, H. and Masuda, S.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 76 Pages: 1248-1251

    • URL

      https://www.jstage.jst.go.jp/article/bbb/76/6/76_120226/_article

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] mRNA 輸送体の多様化とその生物学的意義2012

    • Author(s)
      山崎智弘、増田誠司
    • Journal Title

      化学と生物

      Volume: 50 Pages: 9-11

    • URL

      http://www.jsbba.or.jp/pub/journal_kasei/

    • Related Report
      2012 Final Research Report
  • [Journal Article] mRNA核外輸送の効率化による動物細胞タンパク質生産2012

    • Author(s)
      松村 嘉員
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 70 Pages: 373-375

    • Related Report
      2012 Annual Research Report
  • [Journal Article] mRNA輸送体の多様化とその生物学的意義2012

    • Author(s)
      山崎智弘、増田誠司
    • Journal Title

      化学と生物

      Volume: 50 Pages: 9-11

    • Related Report
      2011 Research-status Report
  • [Journal Article] Enhancing recombinant protein production in human cell lines with a constitutive transport element and mRNA export proteins2011

    • Author(s)
      Aihara Y, Fujiwara N, Yamazaki T, Kambe T, Nagao M, Hirose Y, Masuda S
    • Journal Title

      J Biotechnol

      Volume: 153 Issue: 3-4 Pages: 86-91

    • DOI

      10.1016/j.jbiotec.2011.03.024

    • NAID

      120003072129

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] An extra high dose of erythropoietin fails to support the proliferation of erythropoietin dependent cell lines.2011

    • Author(s)
      Abe, S., Sasaki, R. and Masuda, S.
    • Journal Title

      Cytotechnology

      Volume: 63 Issue: 2 Pages: 101-109

    • DOI

      10.1007/s10616-011-9345-x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Constitutively active soluble form of erythropoietin receptor suppresses growth and angiogenesis of xenografts of transfected cancer cell lines.2011

    • Author(s)
      Yasuda, Y., Maeda, Y., Hara, S., Tanaka, M., Koike, E., Watanabe, Y., Masuda, S., Yamasaki, H., Konishi, H., Horiuchi, Y. and Hoshiai, H.
    • Journal Title

      J. Cancer Therapy

      Volume: 2 Pages: 40-53

    • URL

      http://www.scirp.org/journal/jct/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway2011

    • Author(s)
      Fukunaka, A., Kurokawa, Y., Teranishi, F., Sekler, I., Oda, K., Ackland, ML., Faundez, V., Hiromura, M., Masuda, S., Nagao, M., Enomoto, S. and Kambe, T
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 18 Pages: 16363-16373

    • DOI

      10.1074/jbc.m111.227173

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] mRNA 輸送因子 UAP56 ならびに URH49 における複合体形成制御領域の同定2012

    • Author(s)
      藤田賢一、南裕基、平山瑞季、増田誠司
    • Organizer
      第35回 日本分子生物学会
    • Related Report
      2012 Final Research Report
  • [Presentation] DBP5 の核-細胞質間移行は mRNA 輸送に必須である2012

    • Author(s)
      志岐拓哉、岡村真純、伊藤慶紗、増田誠司
    • Organizer
      第35回 日本分子生物学会
    • Related Report
      2012 Final Research Report
  • [Presentation] Disturbing the function of the nuclear exosome results in the accumulation of adenylated RNA in human nuclei.2012

    • Author(s)
      N. Fujiwara, K. Okumura, T. Fujiwara, S. Masuda
    • Organizer
      the 4th EMBO meeting
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト核内エキソソームはアデニン鎖の付加された RNA 基質を分解する2012

    • Author(s)
      藤原奈央子、程開明、永尾雅哉、神戸大朋、宮前友策、増田誠司
    • Organizer
      第14回日本 RNA 学会年会(第14回 RNA ミーティング)
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA 輸送因子 UAP56 とURH49 の形成する異なる複合体構築機構の解明2012

    • Author(s)
      藤田賢一、宮前友策、永尾雅哉、神戸大朋、増田誠司
    • Organizer
      日本農芸化学大会
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA 輸送因子 DBP5 の核-細胞質間輸送と mRNA 輸送の相関性2012

    • Author(s)
      志岐拓哉、宮前友策、永尾雅哉、神戸大朋、増田誠司
    • Organizer
      日本農芸化学大会
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト核内エキソソームはアデニン鎖の付加されたRNA基質を分解する2012

    • Author(s)
      藤原奈央子
    • Organizer
      第14回日本RNA学会年会
    • Place of Presentation
      東北大学百周年記念会館川内ホール
    • Related Report
      2012 Annual Research Report
  • [Presentation] Disturbing the function of the nuclear exosome results in the accumulation of adenylated RNA in human nuclei2012

    • Author(s)
      Fujiwara N.
    • Organizer
      the 4th EMBO meeting
    • Place of Presentation
      フランス ニース アクロポリスコンベンションセンター
    • Related Report
      2012 Annual Research Report
  • [Presentation] DBP5の核-細胞質間移行はmRNA輸送に必須である2012

    • Author(s)
      志岐拓哉
    • Organizer
      第35回 日本分子生物学会
    • Place of Presentation
      マリンメッセ福岡 1F
    • Related Report
      2012 Annual Research Report
  • [Presentation] mRNA輸送因子UAP56ならびにURH49における複合体形成制御領域の同定2012

    • Author(s)
      藤田賢一
    • Organizer
      第35回 日本分子生物学会
    • Place of Presentation
      マリンメッセ福岡 1F
    • Related Report
      2012 Annual Research Report
  • [Presentation] mRNA輸送因子UAP56とURH49の形成する異なる複合体構築機構の解明2012

    • Author(s)
      藤田賢一、宮前友策、永尾雅哉、 神戸大朋、増田誠司
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都・京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] mRNA輸送因子DBP5の核-細胞質間輸送とmRNA輸送の相関性2012

    • Author(s)
      志岐拓哉、宮前友策、永尾雅哉、 神戸大朋、増田誠司
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都・京都女子大学
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒト核内 RNA 品質管理機構におけるポリアデニル化およびエキソヌクレアーゼ hRrp6 の関与について2011

    • Author(s)
      藤原奈央子、弓立涼介、呉攸、増田誠司
    • Organizer
      平成 23 年度 日本農芸化学会 関西・中部支部合同大会
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA 核外輸送因子 UAP56 と URH49 の形成する複合体の分子基盤解析2011

    • Author(s)
      藤田賢一、栗原朋也、山崎智弘、増田誠司
    • Organizer
      平成 23 年度日本農芸化学会 関西・中部支部合同大会
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA 輸送と mRNA 輸送必須因子 DBP5の核内移行との関係性2011

    • Author(s)
      志岐拓哉、神戸大朋、永尾雅哉、増田誠司
    • Organizer
      日本農芸化学 2011年度大会
    • Related Report
      2012 Final Research Report
  • [Presentation] mRNA 輸送体の多様化と機能分担(シンポジウム、遺伝子動態解析技術の進歩と発現制御)2011

    • Author(s)
      増田誠司
    • Organizer
      日本農芸化学大会
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト核内 RNA品質管理機構における標的 RNA 分子への A付加と分解はエキソソームと RNA ヘリカーゼ MTR4 の相互作用を通じて協調される2011

    • Author(s)
      藤原奈央子、増田誠司
    • Organizer
      第84回日本生化学会大会
    • Related Report
      2012 Final Research Report
  • [Book] mRNA biogenesis in the nucleus and its export to the cytoplasm. "Current Frontiers and Perspectives in Cell Biology", eds by Najman, S.2012

    • Author(s)
      Fujiwara, N., Shiki, T., Masuda, S.
    • Publisher
      InTech - Open Access Publisher
    • Related Report
      2012 Final Research Report
  • [Book] "Cell Biology", eds by Najman, ISBN 979-953-307-505-02012

    • Author(s)
      Fujiwara, N., Shiki, T., Masuda, S.,
    • Publisher
      InTech - Open Access Publisher
    • Related Report
      2011 Research-status Report
  • [Book] 基礎生物学テキストシリーズ「分子生物学」 深見泰夫編、3 章 遺伝情報の発現・転写・プロセシング、4 章 遺伝情報の輸送・翻訳 2011年3月刊行2011

    • Author(s)
      増田誠司
    • Publisher
      化学同人
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/bunshioutou/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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