Molecular mechanisms underlying the reprogramming process of the totipotent fertilized eggs in mammals

• Project/Area Number: 23658292
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Kinki University
• Principal Investigator: MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 分化全能性 / 受精卵 / 初期胚 / リプログラミング / プロテオーム / プロテオーム解析 / 発生・分化制御

Research Abstract

After fertilization, erasure of the oogenic program and reprogramming by establishing the embryonic programs into totipotent zygote are co-ordinately regulated. However, molecular mechanisms underlying the reprogramming process are not well understood. Understanding of the involvement of proteins in the degradation of maternal mRNA and proteins, and also the onset of ZGA at the maternal-to-zygotic transition helps elucidate molecular mechanisms governing the remodeling of the oocyte into the totipotent zygote and may also have implications for regulation of pluripotency.

Research Products

• [Journal Article] GSE is a maternal factor involved in active DNA demethylation in zygotes. (2013)
  • Author(s): Hatanaka Y, Shimizu N, Nishikawa S, Tokoro M, Shin SW, Nishihara T, Amano T, Anzai M, Kato H, Mitani T, Hosoi Y, Kishigami S, Matsumoto K.
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: e60205-e60205
  • DOI: 10.1371/journal.pone.0060205
  • Peer Reviewed
• [Journal Article] Nicotinamide: a Class III HDACi Delays In Vitro Aging of Mouse Oocytes. (2013)
  • Author(s): Lee AR, Kishigami S, Amano T, Matsumoto K, Wakayama T, Hosoi Y.
  • Journal Title: J Reprod Dev.
  • NAID: 10031184752
  • Peer Reviewed
• [Journal Article] Functional Analysis of Nocturnin, a Circadian Deadenylase, at Maternal-to-zygotic Transition in Mice. (2013)
  • Author(s): Nishikawa S, Hatanaka Y, Tokoro M, Shin SW, Shimizu N, Nishihara T, Kato R, Takemoto A, Amano T, Anzai M, Kishigami S, Hosoi Y, Matsumoto K.
  • Journal Title: J Reprod Dev.
  • NAID: 10031184755
  • Peer Reviewed
• [Journal Article] Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition (2013)
  • Author(s): Shin SW
  • Journal Title: Biolology Open Volume: 2 Issue: 2 Pages: 170-182
  • DOI: 10.1242/bio.20123020
  • Peer Reviewed
• [Journal Article] Functional Analysis of Nocturnin, a Circadian Deadenylase, at Maternal-to-zygotic Transition in Mice (2013)
  • Author(s): Nishikawa S
  • Journal Title: J. Rprod. Dev. Volume: 59
  • Peer Reviewed
• [Journal Article] Mouse Zygote-specific Proteasome Assembly Chaperone Important for Maternal-to-zygotic Transition (2013)
  • Author(s): Shin SW
  • Journal Title: Biol. Open Volume: 2 Pages: 170-182
  • Peer Reviewed
• [Journal Article] Growth and development of rabbit oocytes in vitro: effect of fetal bovine serum concentration on culture medium (2012)
  • Author(s): Sugimoto H, Kida Y, Miyamoto Y, Kitada K, Matsumoto K, Saeki K, Taniguchi T, Hosoi Y.
  • Journal Title: Theriogenology Volume: 78(5) Issue: 5 Pages: 1040-1047
  • DOI: 10.1016/j.theriogenology.2012.04.007
  • Peer Reviewed
• [Journal Article] Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei. (2012)
  • Author(s): Yamochi T, Kida Y, Oh N, Ohta S, Amano T, Anzai M, Kato H, Kishigami S, Mitani T, Matsumoto K, Saeki K, Takenoshita M, Iritani A, Hosoi Y.
  • Journal Title: Zygote Volume: 5 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Donor cells at the G1 phase enhance homogeneous gene expression among blastomeres in bovine somatic cell nuclear transfer embryos. (2012)
  • Author(s): Iwamoto D, Kasamatsu A, Ideta A, Urakawa M, Matsumoto K, Hosoi Y, Iritani A, Aoyagi Y, Saeki K.
  • Journal Title: Cell Reprogram. Volume: 14(1) Issue: 1 Pages: 20-28
  • DOI: 10.1089/cell.2011.0035
  • Peer Reviewed
• [Journal Article] Predicting Three-way Interactions of Proteins from Expression Profiles Based on Correlation Coefficient (2012)
  • Author(s): Inoue E, Murakami S, Fujiki T, Yoshihiro T, Takemoto A, Ikegami H, Matsumoto K, Nakagawa M.
  • Journal Title: IPSJ Transactions on Bioinformatics Volume: 5 Issue: 0 Pages: 34-43
  • DOI: 10.2197/ipsjtbio.5.34
  • NAID: 130002124176
  • ISSN: 1882-6679
  • Peer Reviewed
• [Journal Article] Growth and Development of Rabbit Oocytes In Vitro: Effect of Fetal Bovine Serum Concentration on Culture Medium (2012)
  • Author(s): Sugimoto H
  • Journal Title: Theriogenology Volume: 78 Pages: 1040-1047
  • Peer Reviewed
• [Journal Article] Development of Interspecies Cloned Embryos Reconstructed with Rabbit (Oryctolagus Cuniculus) Oocytes and Cynomolgus Monkey (Macaca Fscicularis) Fibroblast Cell Nuclei (2012)
  • Author(s): Yamochi T
  • Journal Title: Zygote Volume: 5 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Donor cells at the g1 phase enhance homogeneous gene expression among blastomeres in bovine somatic cell nuclear transfer embryos. . (2012)
  • Author(s): Iwamoto D, Kasamatsu A, Ideta A, Urakawa M, Matsumoto K, Hosoi Y, Iritani A, Aoyagi Y, Saeki K.
  • Journal Title: Cell Reprogram Volume: 14 Pages: 20-28
  • Peer Reviewed
• [Journal Article] Predicting three-way interactions of proteins from expression profiles based on correlation coefficient. (2012)
  • Author(s): Inoue E., Sho Murakami S, Fujiki T, Yoshihiro T, Takemoto A, Ikegami H, Matsumoto K, Nakagawa M.
  • Journal Title: IPSJ Transactions on Bioinformatics Volume: in press
  • NAID: 130002124176
  • Peer Reviewed
• [Journal Article] Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos (2011)
  • Author(s): Miyamoto, K., Nagai, K., Kitamura, N., Nishikawa, T., Ikegami, H., Binh, N., Tsukamoto, S., Matsumoto, M., Tsukiyama, T., Minami, N., Yamada, M., Ariga, H., Miyake, M., Kawarasaki, T., Matsumoto, K., Imai, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Issue: 17 Pages: 7040-7045
  • DOI: 10.1073/pnas.1013634108
  • Peer Reviewed
• [Presentation] GSE is a maternal factor required for DNA demethylation in early embryos (2012)
  • Author(s): Yuki Hatanaka、Natsumi Shimizu、Kotaro Morita、Satoshi Nishikawa、Takuji Nishihara、RieKato、ChikaHiguchi、Tomoko Amano、Masayuki Anzai、Satoshi Kishigami、Hiromi Kato、Tasuku Mitani、YoshihikoHosoi、Kazuya Matsumoto.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Expression of GSE Proteins in Primordial Germ Cells (2012)
  • Author(s): ohtaroMorita、Yuki Hatanaka、Natsumi Shimizu、SatoshiNishikawa、Takuji Nishihara、RieKato、ChikaHiguchi、Tomoko Amano、Satoshi Kishigami、Hiromi Kato、Tasuku Mitani、Yoshihiko Hosoi、Kazuya Matsumoto.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] The Involvement of Ubiquitin-Proteasome System in Transcription Regulation in Early Mouse Embryos (2012)
  • Author(s): Natsumi Shimizu、Yuki Hatanaka、Chika Higuchi、Seungwook Shin、Satoshi Nishikawa、Takuji Nishihara、RieKato、Atsushi Takemoto、Kotaro Morita、Kohei Nagai、Tomoko Amano、Satoshi Kishigami、Masayuki Anzai、Yoshihiko Hosoi、Kazuya Matsumoto.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] he Involvement of Ubiquitin-Proteasome System in the Major ZGAin Early Mouse Embryos (2012)
  • Author(s): Chika Higuchi、Natsumi Shimizu、Yuki Hatanaka、Seungwook Shin、Satoshi Nishikawa、Takuji Nishihara、Rie Kato、Atsushi Takemoto、Kotaro Morita、Kohei Nagai、Tomoko Amano、Satoshi Kishigami、Masayuki Anzai、Yoshihiko Hosoi、Kazuya Matsumoto.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Identification of a Candidate Factor which is interacting with Histone H3.3 in Early Mouse Embryo (2012)
  • Author(s): ie Kato、Mikiko Tokoro、Satoshi Nishikawa、Yuki Hatanaka、Natsumi Shimizu、Takuji Nishihara、Atsushi Takemoto、Chika Higuchi、Kotaro Morita、Tomoko Amano、SatoshiKishigami、TasukuMitani、Yoshihiko Hosoi、Kazuya Matsumoto.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] PARN Deadenylase Causes Instability of Maternal Poly (A)+ mRNAs in Early Mouse Embryos (2012)
  • Author(s): Satoshi Nishikawa、Yuki Hatanaka、Natsumi Shimizu、Takuji Nishihara、Rie Kato、Atsushi Takemoto、Chika Higuchi、Kotaro Morita、Satoshi Kishigami、TomokoAmano、Kazuhiro Saeki、Yoshihiko Hosoi、Kazuya Matsumoto.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Maternal GSE, germ-cell specific gene, involves in active DNA demethylation in early mouse embryos (2012)
  • Author(s): Yuki Hatanaka, Natsumi Shimizu, Kotaro Morita, Satoshi Nishikawa, Takuji Nishihara, Rie Kato, Atsushi Takemoto, Chika Higuchi, Tomoko Amano, Masayuki Anzai, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Kazuya Matsumoto.
  • Organizer: Germ Cells. Cold Spring Harbor
  • Place of Presentation: New York, USA
• [Presentation] A role of ubiquitin-proteasome system in early mouse embryos (2012)
  • Author(s): Natsumi Shimizu, Yuki Hatanaka, Chika Higuchi, Seung-Wook Shin, Satoshi Nishikawa, Takuji Nishihara, Rie Kato, Atsushi Takemoto, Kohtaro Morita, Tomoko Amano, Satoshi Kishigami, Tasuku Mitani, Kazuhiro Saeki, Yoshihiko Hosoi, Kazuya Matsumoto.
  • Organizer: Germ Cells. Cold Spring Harbor
  • Place of Presentation: New York, USA
• [Presentation] Rhophilin-2はGABARAPと相互作用し、RhoB制御下において初期胚の第一分裂に関与している (2012)
  • Author(s): 守田昂太郎、畑中勇輝、清水なつみ、西川慧、西原卓志、加藤里恵、武本淳史、樋口智香、天野朋子、安齋政幸、岸上哲士、佐伯和弘、細井美彦、松本和也
  • Organizer: 第19回日本胚移植研究会
  • Place of Presentation: 富山大学黒田講堂
• [Presentation] ヒストンシャペロンと考えられる GSEは、マウス受精卵における能動的 DNA脱メチル化に関与している (2012)
  • Author(s): 畑中勇輝、守田昂太郎、清水なつみ、西川慧、西原卓志、加藤里恵、武本淳史、樋口智香、天野朋子、安齋政幸、岸上哲士、佐伯和弘、細井美彦、松本和也
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波大学大学会館
• [Presentation] マウス初期胚におけるユビキチン-プロテアソーム系分解系の役割 (2012)
  • Author(s): 清水なつみ、樋口智香、畑中勇輝、申 承旭、西川 慧、西原 卓志,加藤里恵、武本淳史、守田昂太郎、天野朋子、岸上哲士、佐伯和弘、細井美彦、松本和也
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波大学大学会館
• [Presentation] マウス初期胚における H3.3と相互作用因子の候補である FAF1の機能解析 (2012)
  • Author(s): 加藤里恵, 野老美紀子,西川慧,畑中勇輝,清水なつみ,西原卓志,武本淳史,樋口智香,守田昂太郎, 天野朋子,岸上哲士,佐伯和弘,細井美彦,松本和也
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波大学大学会館
• [Presentation] hophilin-2はGABARAPと相互作用し、RhoB制御下において初期胚の第一分裂に関与している (2012)
  • Author(s): 守田昂太郎、畑中勇輝、清水なつみ、西川慧、西原卓志、加藤里恵、武本淳史、樋口智香、天野朋子、安齋政幸、岸上哲士、佐伯和弘、細井美彦、松本和也
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波大学大学会館
• [Presentation] GSEは受精直後の胚における能動的脱メチル化に関与している。 (2012)
  • Author(s): 畑中勇輝、守田昂太郎、清水なつみ、西村愛美、西川慧、西原卓志、加藤里恵、武本淳史、天野朋子、安齋政幸、岸上哲士、佐伯和弘、細井美彦、松本和也
  • Organizer: 第30回日本受精着床学会大会
  • Place of Presentation: グランキューブ大阪
• [Presentation] マウス初期胚におけるユビキチン・プロテアソーム経路の果たす役割の検討 (2012)
  • Author(s): 清水なつみ, 樋口智香,畑中勇輝,西川 慧, 西原卓志,加藤里恵,武本淳史, 守田昂太郎,天野朋子, 岸上哲士,安齋政幸, 佐伯和弘,細井美彦,松本和也
  • Organizer: 第30回日本受精着床学会大会
  • Place of Presentation: グランキューブ大阪
• [Presentation] マウス初期胚におけるH3.3と相互作用する因子の検討 (2012)
  • Author(s): 加藤里恵,西川慧, 畑中勇輝,清水なつみ,西原卓志, 武本淳史, 守田昂太郎,天野朋子,岸上哲士,佐伯和弘,細井美彦,松本和也
  • Organizer: 第30回日本受精着床学会大会
  • Place of Presentation: グランキューブ大阪
• [Presentation] マウス着床前初期胚におけるpoly(A)mRNAの制御に関する研究 (2012)
  • Author(s): 西川慧,畑中勇輝,清水なつみ,西原卓志,加藤里恵,武本淳史,守田昂太郎, 岸上哲士,天野朋子,佐伯和弘, 細井美彦,松本和也
  • Organizer: 第30回日本受精着床学会大会
  • Place of Presentation: グランキューブ大阪
• [Presentation] GSE is a Maternal Factor Required for DNA Demethylation in Early Embryos. (2012)
  • Author(s): Hatanaka Y
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Expression of GSE Proteins in Primordial Germ Cells (2012)
  • Author(s): Morita K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] The Involvement of Ubiquitin-Proteasome System in Transcription Regulation in Early Mouse Embryos (2012)
  • Author(s): Simizu N
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] The Involvement of Ubiquitin-Proteasome System in the Major ZGA in Early Mouse Embryos (2012)
  • Author(s): Higuchi C
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Involvement of GSE in Reprogramming of Primordial Germ Cells (2011)
  • Author(s): Yuki Hatanaka, Satoshi Nishikawa, Natsumi Shimizu, Takuji Nishihara, Rie Kato, Tomoko Amano, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Kazuya Matsumoto
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] The involvement of ubiquitin-proteasome in the zygotic gene activation in earlymouseembryos (2011)
  • Author(s): Natsumi Shimizu, Seungwook Shin, Hyangheun Lee, Satoshi Nishikawa, Yuki Hatanaka, Takuji Nishihara, Rie Kato, Tomoko Amano, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Kazuya Matsumoto.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Expression and Functional analyses of 5-deadenylase in Early Mouse Embryos (2011)
  • Author(s): Satoshi Nishikawa、Yuki Hatanaka、Natsumi Shimizu、TakujiNishihara、Atsushi Takemoto、Satoshi Kishigami、Tomoko Amano、Kazuhiro Saeki、Yoshihiko Hosoi、Kazuya Matsumoto
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 初期胚及び始原生殖細胞のリプログラミング過程におけるGSE及び新規遺伝子GIAPの発現解析 (2011)
  • Author(s): 畑中勇輝,西川慧,清水なつみ,西原卓志,加藤里恵, 天野朋子,岸上哲士,佐伯和弘,細井美彦,松本和也
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: いわて県民情報交流センター・アイーナ
• [Presentation] ユビキチン-プロテアソーム系のマウス初期胚における転写制御の関与 (2011)
  • Author(s): 清水なつみ, 申承旭,西川慧,畑中勇輝,李香欣,西原卓志,加藤里恵,天野朋子,岸上哲史,佐伯和弘,細井美彦,入谷 明,松本 和也
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: いわて県民情報交流センター・アイーナ
• [Presentation] Inhibition of the ubiquitin-proteasome system leads to delay of the onset of ZGA gene expression in early mouse embryos (2011)
  • Author(s): Seung-Wook Shin, Mikiko Tokoro, Satoshi Nishikawa, Yuki Hatanaka, Hyang-Heun Lee, Natsumi Shimizu, Takuji Nishihara, Rie Kato, Tomoko Amano, Kazuya Matsumoto
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologist
  • Place of Presentation: Okinawa Convention Center
• [Presentation] マウス初期胚におけるH3.3と相互作用候補であるFAF1の検討 (2011)
  • Author(s): 加藤 里恵、野老 美紀子、西川 慧、李 香欣、畑中 勇輝、清水 なつみ、西原 卓志、天野 朋子、佐伯 和弘、細井 美彦、松本 和也
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市いわて県民情報交流センター・アイーナ
• [Presentation] 初期胚およ生殖細胞のリプログラミング過程におけるGSE及び新規遺伝子GIAPの発現解析 (2011)
  • Author(s): 畑中 勇輝、西川 慧、清水 なつみ、西原 卓志、加藤 里恵、天野 朋子、岸上 哲士、佐伯 和弘、細井 美彦、松本 和也
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市いわて県民情報交流センター・アイーナ
• [Presentation] Involvement of GSE in Reprogramming of Primordial Germ Cells (2011)
  • Author(s): Yuki Hatanaka, Satoshi Nishikawa, Natsumi Shimizu, Takuji Nishihara, Rie Kato, Tomoko Amano,Satsi Kisigami, Kazuhiro Saeki, Yoshihiko Hosoi, Kazuya Matsumoto
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
• [Presentation] The involvement of ubiquitin-proteasome in the zygotic gene activation in mouse early embryos (2011)
  • Author(s): Natsumi Shimizu, Seung-Wook Shin, Satoshi Nishikawa, Yuki Hatanaka, Hyang-Heun Lee, Takuji Nishihara, Rie Kato, Tomoko Amano,Satsi Kisigami, Kazuhiro Saeki, Yoshihiko Hosoi, Kazuya Matsumoto
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
• [Book] 胚発生プログラムの遺伝的制御 (2011)
  • Author(s): 松本和也、細井美彦
  • Total Pages: 11
  • Publisher: 京都大学学術出版
• [Book] 胚発生プログラムの遺伝的制御 (2011)
  • Author(s): 松本和也・細井美彦
  • Total Pages: 11
  • Publisher: 京都大学学術出版
• [Patent(Industrial Property Rights)] 牛の判別方法及び牛の判別用キット (2012)
  • Inventor(s): 松本和也、池上春香、武本淳史、小林栄治
  • Industrial Property Rights Holder: 近畿大学、家畜改良センター
  • Industrial Property Number: 2012-008036
  • Filing Date: 2012-01-18
• [Patent(Industrial Property Rights)] 牛の判別方法及び牛の判別用キット (2012)
  • Inventor(s): 松本和也・池上春香・武本淳史・小林栄治
  • Industrial Property Rights Holder: 松本和也・池上春香・武本淳史・小林栄治
  • Industrial Property Number: 2012-008036
  • Filing Date: 2012
• [Patent(Industrial Property Rights)] 牛の判別方法及び牛の判別用キット (2011)
  • Inventor(s): 松本和也、森本康一、池上春香
  • Industrial Property Rights Holder: 近畿大学
  • Industrial Property Number: 2011-263214
  • Filing Date: 2011-12-01
• [Patent(Industrial Property Rights)] 牛の判別方法及び牛の判別用キット (2011)
  • Inventor(s): 松本和也・森本康一・池上春香
  • Industrial Property Rights Holder: 松本和也・森本康一・池上春香
  • Industrial Property Number: 2011-263214
  • Filing Date: 2011