| Project/Area Number |
23659023
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
UNO Tadayuki 大阪大学, 大学院・薬学研究科, 教授 (00183020)
|
|---|
| Research Collaborator |
宮本 正芳 大阪大学, 大学院・薬学研究科, 博士前期課程
野出 純一 大阪大学, 大学院・薬学研究科, 博士前期課程
小泉 温史 大阪大学, 大学院・薬学研究科, 博士前期課程
山田 麻由 大阪大学, 薬学部, 薬学科
小宮山 祐美 大阪大学, 薬学部, 薬学科
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | シトクロムP450 / 薬物代謝 / 一塩基多型 / 電気化学 / 酸化還元電位 / 薬剤反応性 |
|---|
| Research Abstract |
Cytochrome P450 (CYP) is a group of enzymes that play key roles in the drug metabolism in human, but comprehensive studies of CYP have been very difficult, because CYP enzymes contain various isoforms and their drug specificities are quite low. In this study, I aimed at construction of evaluating system for CYP drug metabolism electrochemically. Charging an electric potential for CYP reduction, silver electrode was found to produce silver oxide, and hence I used gold electrode which is more stable than silver. Applying various coating reagents, I found that a mixed solution containing mercaptoundecanoic acid and mercaptoundecanol could result in measurement of redox wave of CYP2C9.
|
