The development in vivo siRNA delivery system for the treatment of renal fibrosis

• Project/Area Number: 23659026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Jichi Medical University
• Principal Investigator: MORISHITA Yoshiyuki 自治医科大学, 医学部, 講師 (30570494)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ドラッグデリバリー / 遺伝子治療 / 腎線維化 / siRNA / ドラッグ・デリバリ-

Research Abstract

1) We detected that smad4-siRNA could significantly inhibit the epithelial mesenchymal transition of HK-2 cells with stimulation of TGF-β1.
2) We intravenously injected modified smad4-siRNA that was added extra bases at 5 prime of antisense strand and at 3 prime of sense strand to increase its stability in vivo (stable-smad4-siRNA) to renal fibrosismice. We detected t

Research Products

• [Journal Article] 腎特異的 siRNA デリバリー技術の開発と腎線維化治療. (2012)
  • Author(s): 森下義幸, 吉澤寛道, 大西央, 草野英二
  • Journal Title: 腎 Volume: 35 Pages: 123-126
• [Journal Article] 腎特異的siRNAデリバリー技術の開発と腎線維化治療 (2012)
  • Author(s): 森下義幸
  • Journal Title: 腎臓 Volume: 35 Pages: 123-126
• [Presentation] Systemic smad4-siRNA administration prevents interstitial fibrosis partially by inhibiting the proliferation of myofibroblasts derived from pericytes in renal fibrosis mouse (2012)
  • Author(s): Morishita Y, Yoshizawa H, Onishi A, Watanabe M, Kusano E
  • Organizer: The 45thAnnual Meeting of the American Society of Nephrology
  • Place of Presentation: sandiego, CA, USA
• [Presentation] Systemic Smad4-siRNA Administration Prevents Interstitial Fibrosis Partially by Inhibiting the Proliferation of Myofibroblasts Derived from Pericytes in Renal Fibrosis Mouse Model (2012)
  • Author(s): Yoshiyuki Morishita
  • Organizer: American Society of Nephrology Kidney Week 2012
  • Place of Presentation: San diego, CA, USA