Approach to reveal dynamics of caspase activation in maintainingbrain homeostasis

• Project/Area Number: 23659032
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAGUCHI Yoshifumi 東京大学, 大学院・薬学系研究科, 助教 (10447443)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳 / カスパーゼ / 神経免疫 / 睡眠

Research Abstract

Caspases are evolutionary conserved cystein protease. In this study, we tried to reveal dynamics of caspase activation in maintaining brain homeostasis. For this purpose, we utilized two novel probes that allow us to detect caspases-1 or caspase-3 in real-time. However, we have not detected weak or mild activation of those caspases in the brain derived from sleep-deprived mouse or in primary cell culture. These results suggest that further improvement in detection method will be needed to observe weak or mild activation of caspases by these probes.

Research Products

• [Journal Article] Masayuki Miura:Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure (2011)
  • Author(s): Yoshifumi Yamaguchi, Naomi Shinotsuka,Keiko Nonomura, Kiwamu Takemoto, Keisuke Kuida,Hiroki Yosida
  • Journal Title: Journal of Cell Biology Volume: 195 Pages: 1047-1060
• [Journal Article] Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure (2011)
  • Author(s): Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Takemoto, K, Kuida, K., Yoshida, H., Miura, M.
  • Journal Title: Journal of Cell Biology Volume: 195 Issue: 6 Pages: 1047-1060
  • DOI: 10.1083/jcb.201104057
  • Peer Reviewed
• [Presentation] COLD SPRING HARBOR ASIA CONFERENCES (2013)
  • Organizer: Mechanisms and Functions of Non-apoptotic Cell Death
  • Place of Presentation: 蘇州・中国
• [Presentation] Visualization of Inflammasome activation: monitoring caspase-1 activity in living cells by a genetically-encoded probe SCAT1 (2012)
  • Author(s): Ting Liu, Yoshifumi Yamaguchi, Koichi Shikada, Katsuaki Hoshino, Tsuneyasu Kaisho, Kiwamu Takemoto, Erina Kuranaga, Masayuki Miura
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-11-14
• [Presentation] Evaluation of SCAT1: a genetically- encoded probe for detecting activation of caspaes- 1 in living cells (2011)
  • Author(s): Koichi Shikada, Yoshifumi Yamaguchi, Ting Liu, Kiwamu Takemoto, Katsuaki Hoshino, Tsuneyasu Kaisho, Erina Kuranaga, Masayuki Miura
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Evaluation of SCAT1: a genetically-encoded probe for detecting activation of caspaes-1 in living cells (2011)
  • Author(s): Koichi Shikada, Yoshifumi Yamaguchi, Ting Liu, Kiwamu Takemoto, Katsuaki Hoshino, Tsuneyasu Kaisho, Erina Kuranaga, Masayuki Miura
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Visualization of Inflammasome Activation: Monitoring Caspase-1 Activation in Living Cells by a Genetically-encoded Probe SCAT1
  • Author(s): Ting Liu
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡