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Establishment of YAP-dependent hepatic tumor formation and global expression analysis of related microRNAs

Research Project

Project/Area Number 23659034
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionTokyo Medical and Dental University

Principal Investigator

NISHINA Hiroshi  東京医科歯科大学, 難治疾患研究所, 教授 (60212122)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
KeywordsYAP / 肝癌 / 細胞競合 / アポトーシス / cDNA / マイクロRNA / HTVi法 / 網羅的遺伝子解析
Research Abstract

Recently, YAP was shown to play an important role in organ size control and to be inhibited by the Hippo signaling pathway. Either transgenic overexpression of YAP or knockout of Hippo pathway genes in mouse liver results in enlargement of this organ and the eventual development of hepatic tumors. In this study, we used the method of hydrodynamic tail vein injection(HTVi) that is specific for gene transfer into liver. As a results, we succeeded in establishing an efficient system of liver cancer induction due to gain of function of the active YAP. Secondly, we analyzed the gene expression by cDNA microarray and identified more than 20 genes whose expression is increased by active YAP. Thus, our results could contribute to elucidate the mechanism of human liver cancer

Report

(2 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (36 results)

All 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 1 results) Presentation (21 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex2011

    • Author(s)
      Tokiwa Yamasaki, Hiroshi Kawasaki, Satoko Arakawa, Kimiko Shimizu, Shigeomi Shimizu, Orly Reiner, Hideyuki Okano, Sachiko Nishina, Noriyuki Azuma, Josef M. Penninger, Toshiaki Katada and Hiroshi Nishina
    • Journal Title

      J. Neurosci

      Volume: 31 Pages: 16872-16883

    • Related Report
      2011 Final Research Report
  • [Journal Article] Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver2011

    • Author(s)
      Norio Miyamura^1, Takashi Nakamura^1, Naoko Goto-Inoue, Nobuhiro Zaima, Takahiro Hayasaka, Tokiwa Yamasaki, Shuji Terai, Isao Sakaida
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 408 Pages: 120-125

    • Related Report
      2011 Final Research Report
  • [Journal Article] Diphenyleneiodonium chloride, an inhibitor of NADPH oxidase, suppresses light-dependent induction of clock and DNA repair genes in zebrafish2011

    • Author(s)
      Tomomi Osaki, Yoshimi Uchida, Jun Hirayama, and Hiroshi Nishina
    • Journal Title

      Biol. Pharm. Bull

      Volume: 34 Pages: 1343-1347

    • Related Report
      2011 Final Research Report
  • [Journal Article] RASSF7 negatively regulates pro-apoptotic JNK signaling by inhibiting the activity of phospholylated-MKK72011

    • Author(s)
      Shinya Takahashi, Arisa Ebihara, Hiroaki Kajiho, Kenji Kontani, Hiroshi Nishina, and Toshiaki Katada
    • Journal Title

      Cell Death Differ

      Volume: 18 Pages: 645-655

    • Related Report
      2011 Final Research Report
  • [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs2011

    • Author(s)
      Shinya Takahashi, Kyoko Sakurai, Arisa Ebihara, Hiroaki Kajiho, Kota Saito, Kenji Kontani, Hiroshi Nishina, and Toshiaki Katada
    • Journal Title

      Nucleic Acids Res

      Volume: 39 Pages: 3446-3457

    • Related Report
      2011 Final Research Report
  • [Journal Article] Autotaxin regulates vascular development via multiple lysophosphatidic acid(LPA) receptors in zebrafish2011

    • Author(s)
      Hiroshi Yukiura, Kotaro Hama, Keita Nakanaga, Masayuki Tanaka, Yoichi Asaoka, Shinichi Okudaira, Naoaki Arima, Asuka Inoue, Takafumi Hashimoto, Hiroyuki Arai, Atsuo Kawahara, Hiroshi Nishina, and Junken Aoki
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 43972-43983

    • Related Report
      2011 Final Research Report
  • [Journal Article] TNFR1 mediated signaling is important to induce the improvement of liver fibrosis by bone marrow cell infusion2011

    • Author(s)
      Takuro Hisanaga, Shuji Terai, Takuya Iwamoto, Taro Takami, Naoki Yamamoto, Tomoaki Murata, Toshifumi Matsuyama, Hiroshi Nishina, Isao Sakaida
    • Journal Title

      Cell Tissue Res

      Volume: 346 Pages: 79-88

    • Related Report
      2011 Final Research Report
  • [Journal Article] Telmisartan improved nonalcoholic steatohepatitis in medaka(Oryzias latipes) by reducing macrophage infiltration and fat accumulation2011

    • Author(s)
      Shinya Kuwashiro, Shuji Terai, Toshiyuki Oishi, Fujisawa Koichi, Toshihiko Matsumoto, Hiroshi Nishina, Isao Sakaida
    • Journal Title

      Cell Tissue Res

      Volume: 344 Pages: 125-134

    • Related Report
      2011 Final Research Report
  • [Journal Article] A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription2011

    • Author(s)
      Yijun Bao, Kentaro Nakagawa, Zeyu Yang, Mitsunobu Ikeda, KanchanamalaWithanage, Mari Ishigami-Yuasa, Yukiko Okuno, Shoji Hata, Hiroshi Nishina, and Yutaka Hata
    • Journal Title

      J. Biochem

      Volume: 150 Pages: 199-208

    • NAID

      10030587010

    • Related Report
      2011 Final Research Report
  • [Journal Article] メダカとゼブラフィッシュを用いた肝研究2011

    • Author(s)
      浅岡洋一、仁科博史
    • Journal Title

      実験医学

      Volume: Vol.29(No.13) Pages: 2090-2095

    • Related Report
      2011 Final Research Report
  • [Journal Article] モデル生物-マウスと小型魚類:トランスポートソームの世界-膜輸送研究の源流から未来へ-2011

    • Author(s)
      仁科博史
    • Journal Title

      京都廣川書店(執筆分担)

      Pages: 434-439

    • Related Report
      2011 Final Research Report
  • [Journal Article] Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver2011

    • Author(s)
      Miyamura N, Nakamura T, Goto-Inoue N, Zaima N, Hayasaka T, Yamasaki T, Terai S, Sakaida I, Setou M, Nishina H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 408 Issue: 1 Pages: 120-125

    • DOI

      10.1016/j.bbrc.2011.03.133

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] RASSF7 functions as an anti-apoptotic regulator of JNK signaling by inhibiting phosphorylated MKK7 activity2011

    • Author(s)
      Hiroshi Nishina
    • Organizer
      2nd RASSF Symposium
    • Place of Presentation
      Oxford, UK
    • Year and Date
      2011-07-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Light-dependent regulation of zebrafish circadian transcription[ Spin Chemistry Meeting 20112011

    • Author(s)
      Hirayama Jun, et al
    • Organizer
      Noordwijk
    • Place of Presentation
      Netherlands
    • Related Report
      2011 Final Research Report
  • [Presentation] RASSF7 functions as an anti-apoptotic regulator of JNK signaling by inhibiting phosphorylated MKK7 activity[ 2nd RASSF Symposium2011

    • Author(s)
      Hiroshi Nishina
    • Organizer
      Oxford
    • Place of Presentation
      UK
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of novel kinase phosphorylating CRYPTOCHROME1to regulate its protein stability[ 5th Asia and Oceania Conference for Photobiology2011

    • Author(s)
      Hirayama Jun, et al
    • Organizer
      Nara
    • Place of Presentation
      Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver[ Liver Down Under 20112011

    • Author(s)
      Hiroshi Nishina
    • Organizer
      Perth
    • Place of Presentation
      Australia
    • Related Report
      2011 Final Research Report
  • [Presentation] Involvement of the Stress Kinase MKK7 in the regulation of the mammalian circadian clock[ GCOE International Symposium2011

    • Author(s)
      Yoshimi Uchida, et al
    • Organizer
      Designing the circadian clock
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] 肝再生時の脂質代謝の解析2011

    • Author(s)
      宮村憲央, 他
    • Organizer
      第14回日本肝臓医生物学研究会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 質量顕微鏡を用いた再生肝における脂質量変化の解析2011

    • Author(s)
      宮村憲央, 他
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Presentation] ストレス応答性JNKシグナル経路による分子時計制御機構の解析2011

    • Author(s)
      尾崎友美, 他
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞死制御因子DAXXによる分子時計制御2011

    • Author(s)
      平山順
    • Organizer
      日本分子生物学会第11回春季シンポジウム
    • Place of Presentation
      金沢
    • Related Report
      2011 Final Research Report
  • [Presentation] がん原遺伝子産物YAPの新規翻訳後修飾アセチル化の同定2011

    • Author(s)
      畠星治, 他
    • Organizer
      日本分子生物学会第11回春季シンポジウム
    • Place of Presentation
      金沢
    • Related Report
      2011 Final Research Report
  • [Presentation] ストレス応答性キナーゼJNKによる分子時計制御因子BMAL1のリン酸化2011

    • Author(s)
      尾崎友美, 他
    • Organizer
      日本分子生物学会第11回春季シンポジウム
    • Place of Presentation
      金沢
    • Related Report
      2011 Final Research Report
  • [Presentation] がん原遺伝子産物YAPの新規翻訳後修飾アセチル化の同定2011

    • Author(s)
      畠星治, 他
    • Organizer
      第10回生命科学研究会
    • Place of Presentation
      高崎
    • Related Report
      2011 Final Research Report
  • [Presentation] 質量顕微鏡を用いたマウス再生肝の脂質の解析2011

    • Author(s)
      宮村憲央, 他
    • Organizer
      第18回肝細胞研究会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞の生死を制御するストレス応答性MAPキナーゼシグナル伝達系2011

    • Author(s)
      仁科博史
    • Organizer
      第20回日本Cell Death学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] ストレス応答性キナーゼMKK7による軸索伸長制御2011

    • Author(s)
      山崎世和, 他
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report
  • [Presentation] RASSF familyによるストレス応答性JNKシグナルの制御2011

    • Author(s)
      仁科博史
    • Organizer
      第84回日本生化学大会シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 癌遺伝子YAPによる肝癌誘発系の確立2011

    • Author(s)
      宮村憲央, 他
    • Organizer
      第15回日本肝臓医生物学研究会
    • Place of Presentation
      秋田
    • Related Report
      2011 Final Research Report
  • [Presentation] ストレス応答性キナーゼMKK7による分子時計制御機構の解明2011

    • Author(s)
      内田好海, 他
    • Organizer
      第18回日本時間生物学会学術大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] Recent Advances of Hippo and RASSF Signaling Pathways that Regulates Cell Fate2011

    • Author(s)
      仁科博史/佐々木洋世話人ワークショップ
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report
  • [Presentation] 小型魚類を用いた肝臓研究2010

    • Author(s)
      仁科博史
    • Organizer
      第1回医学・創薬に向けた小型魚類モデル利用推進ネットワーク
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Book] Hippo pathway癌・細胞死・再生の新たな鍵を握る器官サイズ制御シグナル2011

    • Author(s)
      畑裕/仁科博史監修
    • Publisher
      秀潤社
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/mri/dbio/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/mri/dbio/index.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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