| Project/Area Number |
23659040
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
DOI Takefumi 大阪大学, 薬学研究科, 教授 (00211409)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKADA Yoshiaki 大阪大学, 薬学研究科, 准教授 (50444500)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 組織特異的遺伝子発現 / Robo4遺伝子 / DNAメチル化 / エピジェネティクス / 血管内皮細胞 / SP1 / GABP |
|---|
| Research Abstract |
Gene expression of the genomic DNA is tightly regulated. Some genes are specifically expressed in particular tissues, although it is not clearly understood how tissue-specific gene expression is regulated. Previous research on skeletal muscle has evidently shown that the skeletal muscle-specific transcription factor MyoD regulates skeletal muscle-specific genes. However, master regulators similar to MyoD have not been identified in most other tissues, suggesting that tissue-specific gene expression is regulated by other mechanisms as well. To investigate these putative mechanisms, we chose the endothelial cell (EC)-specific gene Robo4 and studied the regulation of its gene expression at the transcriptional level, which occurs via transcription factors and epigenetic controls.
|
