Project/Area Number |
23659040
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biological pharmacy
|
Research Institution | Osaka University |
Principal Investigator |
DOI Takefumi 大阪大学, 薬学研究科, 教授 (00211409)
|
Co-Investigator(Kenkyū-buntansha) |
OKADA Yoshiaki 大阪大学, 薬学研究科, 准教授 (50444500)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 組織特異的遺伝子発現 / Robo4遺伝子 / DNAメチル化 / エピジェネティクス / 血管内皮細胞 / SP1 / GABP |
Research Abstract |
Gene expression of the genomic DNA is tightly regulated. Some genes are specifically expressed in particular tissues, although it is not clearly understood how tissue-specific gene expression is regulated. Previous research on skeletal muscle has evidently shown that the skeletal muscle-specific transcription factor MyoD regulates skeletal muscle-specific genes. However, master regulators similar to MyoD have not been identified in most other tissues, suggesting that tissue-specific gene expression is regulated by other mechanisms as well. To investigate these putative mechanisms, we chose the endothelial cell (EC)-specific gene Robo4 and studied the regulation of its gene expression at the transcriptional level, which occurs via transcription factors and epigenetic controls.
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