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Detection of oxidized ERprotein by nitrosative/oxidative stress with a specific antibody

Research Project

Project/Area Number 23659041
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionOkayama University

Principal Investigator

UEHARA Takashi  岡山大学, 大学院・医歯薬学総合研究科, 教授 (00261321)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords一酸化窒素 / 酸化ストレス / 神経細胞 / アポトーシス / 神経変性疾患 / シグナル伝達 / 特殊抗体 / 過酸化水素 / 神経細胞死 / 検出 / 抗体 / 細胞死 / 小胞体ストレス / 変性蛋白質
Research Abstract

The aim of this study is to establish the assay system to detect oxidized PDI (PDI-SO3H) with a specific antibody. We succeeded to isolate several specific antibodies against PDI-SO3H. An antibody could detect the oxidized form of PDI treated with H2O2, NO, or rotenone. This antibody might be useful for diagnosis of sporadic neurodegenerative disprders such as Alzheimer’s and Parkinson’s diseases.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (40 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (13 results) (of which Invited: 3 results) Book (12 results) Remarks (10 results)

  • [Journal Article] Participation of metabotropic glutamate receptors in pentetrazol-induced kindled seizure2011

    • Author(s)
      Watanabe, Y., Kaida, Y., Fukuhara, S., Takechi, K., Uehara, T., and Kamei, C
    • Journal Title

      Epilepsia

      Volume: 52 Issue: 1 Pages: 140-150

    • DOI

      10.1111/j.1528-1167.2010.02764.x

    • Related Report
      2012 Final Research Report
  • [Journal Article] On-off system for PI3-kinase-Akt signaling through S-nitrosylation of phosphatase with sequence homology to tensin (PTEN)2011

    • Author(s)
      Numajiri N., Takasawa K., Nishiya T., Tanaka H., Ohno K., Hayakawa W., Asada M., Matsuda H., Azumi K., Kamata H., Nakamura T., Hara H., Minami M., Lipton S.A., Uehara T.
    • Journal Title

      Proceedings of the National Academy of Sciences, USA

      Volume: 108 Issue: 25 Pages: 10349-10354

    • DOI

      10.1073/pnas.1103503108

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Screening systems for identification of S-nitrosylated proteins2011

    • Author(s)
      Uehara, T. and Nishiya, T
    • Journal Title

      Nitric Oxide

      Volume: 25 Issue: 2 Pages: 108-111

    • DOI

      10.1016/j.niox.2010.11.002

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The ECS (SPSB) E3 ubiquitin ligase is the master regulator of the lifetime of inducible nitric-oxide synthase. Biochem2011

    • Author(s)
      Matsumoto, K., Nishiya, T., Maekawa, S., Horinouchi, T., Ogasawara, K., Uehara, T., and Miwa, S
    • Journal Title

      Biophys. Res. Commun

      Volume: 409 Issue: 1 Pages: 46-51

    • DOI

      10.1016/j.bbrc.2011.04.103

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] SPRY domain-containing SOCS box protein-1 (SPSB1), SPSB2, and SPSB4 are master regulators of proteasome-dependent degradation of iNOS2011

    • Author(s)
      Nishiya, T., Matsumoto, K., Maekawa, S., Horinouchi, T., Fujimuro, M., Ogasawara, K., Uehara, T., and Miwa, S
    • Journal Title

      J. Biol. Chem

      Volume: 286 Issue: 11 Pages: 9009-9019

    • DOI

      10.1074/jbc.m110.190678

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] ガス状分子の神経細胞死に対する作用機構2012

    • Author(s)
      上原 孝
    • Organizer
      第39回日本毒性学会学術年会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2012-07-19
    • Related Report
      2012 Final Research Report
  • [Presentation] NOによる蛋白質修飾と神経細胞死制御2012

    • Author(s)
      上原 孝
    • Organizer
      第53回日本生化学会
    • Place of Presentation
      中国・四国支部例会 岡山大学
    • Year and Date
      2012-05-18
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel modulatory 'on-off' system for PI 3-kinase-Akt signaling through S-nitrosylation2011

    • Author(s)
      上原 孝
    • Organizer
      第11回日本NO学会学術集会
    • Place of Presentation
      昭和薬科大学
    • Year and Date
      2011-05-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗体アレイを使用した新規S-ニトロシル化蛋白質の探索2010

    • Author(s)
      上原 孝
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of oxidized protein-disulfide isomerase with a specific antibody2010

    • Author(s)
      Takashi Uehara
    • Organizer
      40thAnnual Meeting Neuroscience 2010
    • Place of Presentation
      San Diego Convention Centre
    • Year and Date
      2010-11-15
    • Related Report
      2012 Final Research Report
  • [Presentation] ニトロソ化ストレスを切り口とした神経変性疾患の早期診断/治療薬開発へのアプローチ2010

    • Author(s)
      上原 孝
    • Organizer
      生体機能と創薬シンポジウム 2010
    • Place of Presentation
      京都 グランヴィア京都
    • Year and Date
      2010-09-09
    • Related Report
      2012 Final Research Report
  • [Presentation] The life time of iNOS is regulated by the SPRY domain-containing SOCS box protein family linking iNOS to the elongin BC-Cul5-Rbx2 E3 ubiquitin ligase complex2010

    • Author(s)
      Tadashi Nishiya, Satoshi Maekawa, Masahiro Fujimuro, Kouetsu Ogasawara, Takashi Uehara, and Soichi Miwa
    • Organizer
      The 6thInternational Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric oxide
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of oxidized ER protein by nitrosative/oxidative stress with a specific antibody2010

    • Author(s)
      Takashi Uehara
    • Organizer
      The 6thInternational Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric oxide
    • Place of Presentation
      京都
    • Year and Date
      2010-06-16
    • Related Report
      2012 Final Research Report
  • [Presentation] NOによる蛋白質修飾と神経細胞死制御

    • Author(s)
      上原 孝
    • Organizer
      第53回日本生化学会 中国・四国支部例会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] A novel modulatory “on-off”system for PI 3-kinase-Akt signaling through S-nitrosylation of PTEN

    • Author(s)
      Takashi Uehara
    • Organizer
      The 33rd Naito Conference on Oxygen Biology
    • Place of Presentation
      Chateraise Gateaux Kingdom Sapporo
    • Related Report
      2012 Annual Research Report
  • [Presentation] ガス状分子の神経細胞死に対する作用機構

    • Author(s)
      上原 孝
    • Organizer
      第39回日本毒性学会学術年会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 一酸化窒素によるAkt シグナリング調節機構

    • Author(s)
      上原 孝
    • Organizer
      第12回分子予防環境医学研究会
    • Place of Presentation
      つくばサイエンス・インフォメーションセンター
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] タンパク質S-ニトロシル化/酸化を介したによる小胞体ストレス誘導機構

    • Author(s)
      柴田万里
    • Organizer
      日本薬学会第133回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Annual Research Report
  • [Book] 一酸化窒素(NO)結合性(S-ニトロシル化)タンパク質の網羅的同定2013

    • Author(s)
      上原 孝
    • Total Pages
      6
    • Publisher
      生化学
    • Related Report
      2012 Final Research Report
  • [Book] 小胞体内ニトロシル化による神経変性疾患の発症,脳2013

    • Author(s)
      上原 孝
    • Total Pages
      6
    • Publisher
      金芳堂
    • Related Report
      2012 Final Research Report
  • [Book] 脳212013

    • Author(s)
      上原 孝
    • Total Pages
      6
    • Publisher
      金芳堂
    • Related Report
      2012 Annual Research Report
  • [Book] 生化学2013

    • Author(s)
      上原 孝
    • Total Pages
      6
    • Publisher
      日本生化学会
    • Related Report
      2012 Annual Research Report
  • [Book] タンパク質ニトロシル化による細胞生死調節,実験医学2012

    • Author(s)
      上原 孝
    • Total Pages
      7
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 発癌/神経細胞の生死に関わる細胞内ROSシグナル制御,細胞工学2012

    • Author(s)
      鎌田英明,上原 孝
    • Total Pages
      6
    • Publisher
      秀潤社
    • Related Report
      2012 Final Research Report
  • [Book] 細胞工学2012

    • Author(s)
      鎌田英明
    • Total Pages
      6
    • Publisher
      秀潤社
    • Related Report
      2012 Annual Research Report
  • [Book] 実験医学2012

    • Author(s)
      上原 孝
    • Total Pages
      7
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Book] 細胞工学2012

    • Author(s)
      鎌田英明,上原 孝
    • Publisher
      秀潤社
    • Related Report
      2011 Research-status Report
  • [Book] NOはPI3-キナーゼ-Aktシグナルのスイッチ分子として機能している,実験医学2011

    • Author(s)
      上原 孝
    • Total Pages
      4
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 実験医学2011

    • Author(s)
      上原 孝
    • Publisher
      羊土社
    • Related Report
      2011 Research-status Report
  • [Book] NO・ニトロソ化シグナルと細胞死,実験医学2009

    • Author(s)
      上原 孝
    • Total Pages
      5
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月7日掲載,山陽新聞(27面)「NO濃度細胞生死の鍵/脳梗塞新薬に期待」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月7日放送,RSK山陽放送RSKニュース「脳梗塞に新たな治療の可能性」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月7日放送,KSB瀬戸内海放送KSBニュース「脳こうそくの治療に期待世界初の発見」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月7日WEB掲載,東京新聞「細胞生死の鍵はNO,治療応用も」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月7日WEB掲載,福井新聞「細胞生死の鍵はNO,治療応用も」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月16日掲載,朝日新聞(29面)「一酸化窒素濃度脳細胞生死の鍵」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月17日掲載,科学新聞(4面)「細胞生死の鍵握る一酸化窒素2面性の作用機構解明ガンなどの新たな治療薬も」

    • Related Report
      2012 Final Research Report
  • [Remarks] 報道関連情報,平成23年6月19日掲載,読売新聞(31面)「細胞の保護・破壊濃度次第一酸化窒素の作用解明」

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/yakuri/Uehara_Lab/Welcome.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 岡山大学大学院医歯薬学総合研究科薬効解析学分野

    • URL

      http://pharm.okayama-u.ac.jp/lab/yakuri/Uehara_Lab/Welcome.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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