Detection of oxidized ERprotein by nitrosative/oxidative stress with a specific antibody
| Project/Area Number |
23659041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Okayama University |
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Principal Investigator |
UEHARA Takashi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (00261321)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 一酸化窒素 / 酸化ストレス / 神経細胞 / アポトーシス / 神経変性疾患 / シグナル伝達 / 特殊抗体 / 過酸化水素 / 神経細胞死 / 検出 / 抗体 / 細胞死 / 小胞体ストレス / 変性蛋白質 |
|---|
| Research Abstract |
The aim of this study is to establish the assay system to detect oxidized PDI (PDI-SO3H) with a specific antibody. We succeeded to isolate several specific antibodies against PDI-SO3H. An antibody could detect the oxidized form of PDI treated with H2O2, NO, or rotenone. This antibody might be useful for diagnosis of sporadic neurodegenerative disprders such as Alzheimer’s and Parkinson’s diseases.
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Report
(3 results)
Research Products
(40 results)
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[Journal Article] Participation of metabotropic glutamate receptors in pentetrazol-induced kindled seizure.2011
Author(s)
Watanabe, Y., Kaida, Y., Fukuhara, S., Takechi, K., Uehara, T., and Kamei, C
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Journal Title
Epilepsia
Volume: 52
Pages: 140-150
DOI
Related Report
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[Journal Article] On-off system for PI3-kinase-Akt signaling through S-nitrosylation of phosphatase with sequence homology to tension2011
Author(s)
Numajiri N., Takasawa K., Nishiya T., Tanaka H., Ohno K., Hayakawa W., Asada M., Matsuda H., Azumi K., Kamata H., Nakamura T., Hara H., Minami M., Lipton S.A., Uehara T.
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Journal Title
Proc. Natl. Acad. Sci. U.S.A.
Volume: 108
Pages: 10349-10354
DOI
Related Report
Peer Reviewed
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[Journal Article] The ECS(SPSB) E3 ubiquitin ligase is the master regulator of the lifetime of inducible nitric-oxide synthase2011
Author(s)
Matsumoto, K., Nishiya, T., Maekawa, S., Horinouchi, T., Ogasawara, K., Uehara, T., and Miwa, S
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 409
Pages: 46-51
DOI
Related Report
Peer Reviewed
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[Journal Article] SPRY domain-containing SOCS box protein-1 (SPSB1), SPSB2, and SPSB4 are master regulators of proteasome-dependent degradation of iNOS.2011
Author(s)
Nishiya, T., Matsumoto, K., Maekawa, S., Horinouchi, T., Fujimuro, M., Ogasawara, K., Uehara, T., and Miwa, S
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Journal Title
J Biol Chem
Volume: 286
Pages: 9009-19
DOI
Related Report
Peer Reviewed
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[Book] 生化学2013
Author(s)
上原 孝
Total Pages
6
Publisher
日本生化学会
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